Polysaccharide Extracted from Bletilla striata Promotes Proliferation and Migration of Human Tenocytes

Abstract

Tendon healing after injury is relatively slow, mainly because of the weak activity and metabolic properties of tendon cells (tenocytes). Bletilla striata polysaccharide (BSP) has been reported to enhance cell proliferation. Here, we aimed to increase tendon cell proliferation by BSP treatment. We isolated tenocytes from the flexor tendon of human origin. Moreover, we improved the process of extracting BSP. When human tenocytes (HTs) were treated with 100 μg/mL BSP, the MEK/ERK1/2 and PI3K/Akt signaling pathways were activated, thereby enhancing the proliferation ability of tenocytes. BSP treatment also increased the migration of HTs and their ability to secrete the extracellular matrix (Col-I and Col-III). In conclusion, BSP was successfully extracted from a natural Chinese herbal extract and was shown to enhance tenocytes proliferation, migration and collagen release ability. This study is the first to demonstrate improved healing of tendons using BSP.

To Synthesize Hydroxyapatite by Modified Low Temperature Method Loaded with Bletilla striata Polysaccharide as Antioxidant for the Prevention of Sarcopenia by Intramuscular Administration

“…Via endocytosis, BSP might be released into the cytoplasm and eventually into the extracellular space due to a high level of Ca 2+ ; BSP might enter the circulatory system by diffusion and be transported throughout the body. In previous studies, Bletilla striata polysaccharide has been found have good anti-inflammatory and antioxidant effects to play important roles in endothelial cell proliferation, inducible nitric oxide stimulation, wound healing acceleration and other processes [ 14 , 23 ]. The antioxidant effect was also confirmed that the developed BSP-HAP could suppress LPS-induced ROS generation ( Figure 7 ).…”

“…f. (Orchidaceae) is a traditional herbal medicine. In recent studies, a polysaccharide extracted from Bletilla striata (BSP) has been reported to have anti­oxidative and anti-inflammatory properties [ 12 , 13 , 14 ]. BSP is an effective scavenger of reactive oxygen species (ROS) and can downregulate the expression of inflammatory factors such as tumor necrosis factor (TNF- ) and interleukin 6 (IL-6) [ 15 , 16 ].…”

Modified Low-Temperature Extraction Method for Isolation of Bletilla striata Polysaccharide as Antioxidant for the Prevention of Alzheimer’s Disease

“…The data were expressed as mean ± standard deviation (SD). All statistical analyses were done using Student’s t -test or one-way analysis of variance (ANOVA) post hoc tests and by Tukey’s test using GraphPad Prism software (Prism 9, GraphPad Software, Inc., La Jolla, CA, USA), where p -values of less than 0.05 were considered statistically significant [ 76 ].…”

Extracellular Vesicles of Adipose-Derived Stem Cells Promote the Healing of Traumatized Achilles Tendons

“…The proliferation and migration of tenocytes are believed to play an important role in tendon healing [52] and are related to the strength of the healed tendons [53][54][55][56]. In our previous study, human tenocytes treated with polysaccharides extracted from Bletilla striata demonstrated better migration and enhanced proliferation via activation of MEK/ERK1/2 and PI3K/Akt signaling pathways, as well as in the secretion of extracellular matrix [57]. Similarly, bone marrow MSC-derived conditioned medium enhances the proliferation and migration of tenocytes, leading to improved healing of tendons [58].…”