Preparation and evaluation of acellular sheep periostea for guided bone regeneration

Chen

Hong

et al. 2022

Bioactive Materials

330

263

The application of scaffolding materials is believed to hold enormous potential for tissue regeneration. Despite the widespread application and rapid advance of several tissue-engineered scaffolds such as natural and synthetic polymer-based scaffolds, they have limited repair capacity due to the difficulties in overcoming the immunogenicity, simulating in-vivo microenvironment, and performing mechanical or biochemical properties similar to native organs/tissues. Fortunately, the emergence of decellularized extracellular matrix (dECM) scaffolds provides an attractive way to overcome these hurdles, which mimic an optimal non-immune environment with native three-dimensional structures and various bioactive components. The consequent cell-seeded construct based on dECM scaffolds, especially stem cell-recellularized construct, is considered an ideal choice for regenerating functional organs/tissues. Herein, we review recent developments in dECM scaffolds and put forward perspectives accordingly, with particular focus on the concept and fabrication of decellularized scaffolds, as well as the application of decellularized scaffolds and their combinations with stem cells (recellularized scaffolds) in tissue engineering, including skin, bone, nerve, heart, along with lung, liver and kidney.

Section: Decm Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Section: Decm Scaffolds For Tissue Engineeringmentioning

confidence: 99%

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Chen

Hong

et al. 2022

Bioactive Materials

330

263

“…SIS is considered an attractive candidate for periosteum tissue engineering construction due to its elastic, immunocompatible, and biodegradable properties [8,9]. In recent years, acellular dermis, such as amniotic membrane, porcine membrane and human acellular materials, have been used to replace periosteum and to treat bone defects [10][11][12]. It is a simple and convenient method to simulate periosteum tissue by covering the bone defect with a multipotent stem cells sheet.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Silk Fibroin/Sericin Composite Film: Preparation, Mechanical Properties and Mineralization Activity

Tian

et al. 2022

Polymers

The periosteum plays an important role in bone formation and reconstruction. One of the reasons for the high failure rate of bone transplantation is the absence of the periosteum. Silk fibroin (SF) and silk sericin (SS) have excellent biocompatibility and physicochemical properties, which have amazing application prospects in bone tissue engineering, but lacked mechanical properties. We developed a series of SF/SS composite films with improved mechanical properties using boiling water degumming, which caused little damage to SF molecular chains to retain larger molecules. The Fourier transform infrared spectroscopy and X-ray diffraction results showed that there were more β-sheets in SF/SS films than in Na2CO3 degummed SF film, resulting in significantly improved breaking strength and toughness of the composite films, which were increased by approximately 1.3 and 1.7 times, respectively. The mineralization results showed that the hydroxyapatite (HAp) deposition rate on SF/SS composite films was faster than that on SF film. The SF/SS composite films effectively regulated the nucleation, growth and aggregation of HAp-like minerals, and the presence of SS accelerated the early mineralization of SF-based materials. These composite films may be promising biomaterials in the repair and regeneration of periosteum.

“…There are several decellularization methods to remove the cellular components within tissues/organs involving physical, chemical, enzymatic, or a combination protocol 27 . In the present experiment, a combination of physical, chemical, and enzymatic treatment is confirmed after pretest studies repeatedly.…”

Section: Discussionmentioning

confidence: 52%

Manufacture and preliminary evaluation of acellular tooth roots as allografts for alveolar ridge augmentation

Liu

J Biomedical Materials Res

et al. 2021

Self Cite

Alveolar ridge augmentation can be used to obtain appropriate alveolar ridge for dental implantation. A variety of bone graft materials including autogenous bone, allograft, xenograft, and alloplastic material are used in alveolar ridge augmentation. Autogenous tooth‐derived bone graft material has received much attention for the past few years, because the structure and physicochemical characteristics of tooth are similar to those of bones. Compared to autogenous tooth, allogenic tooth has the advantage of extensive resources. However, the problem of cell‐derived immunological rejection of allogenic tooth remains unresolved. In the present study, acellular tooth root (ATR) is obtained by an innovative combination procedure. The biocompatibility of ATR is assessed using cytotoxicity test, hemolysis test, intracutaneous reactivity test, and acute systemic toxicity test. Osseointegration is evaluated in vivo by implanting ATR into the rat tibia defect as an allograft material. The results show that the ATR has fine biocompatibility, and there is an osseointegration between ATR and bone bed at 8 weeks post operation. This study demonstrates that the ATR could be used in alveolar ridge augmentation as a kind of new tooth‐derived bone graft material.