Preparation and evaluation of a molecularly imprinted polymer for the selective recognition of testosterone—application to molecularly imprinted sorbent assays

Abstract

Biomimetic testosterone receptors were synthesized via molecular imprinting for use as antibody mimics in immunoassays. As evaluated by radioligand binding assays, imprinted polymers prepared in acetonitrile were very specific for testosterone because the nonimprinted control polymers bound virtually no radiolabeled testosterone. The polymers present an appreciable affinity, with association constants of K(a) = 3.3 x 10(7) M(- 1) (high-affinity binding sites). The binding characteristics of the polymers were also evaluated in aqueous environment to study their viabilities as alternatives to antibodies in molecularly imprinted sorbent assays. Compared with the testosterone-specific antibodies present in commercial kits, our molecularly imprinted polymers are somewhat less sensitive but show a high selectivity.

Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging

“…At the same time,w ev erified that there was no self-initiated polymerization, ap henomenon frequently observed in the presence of numerous monomers and under lower-wavelength UV light. [17,18] MIPs are tailormade synthetic antibody mimics that can recognize and bind target molecules specifically.They are synthesized by copolymerizing functional and cross-linking monomers in the presence of am olecular template,t hus resulting in the formation of binding sites with affinities and specificities comparable to those of natural antibodies.T heir molecularrecognition properties,c ombined with ah igh chemical and physical stability,m ake them interesting substitutes for antibodies in immunoassays, [19] biosensors, [20] bioseparation, [18,21] controlled drug release, [22] and bioimaging. [12][13][14][15] Consequences include effects on tumor growth, escape from apoptosis,m etastasis formation, and resistance to therapy.P olysaccharides involved in the glycosylation procedure have ah ighly conserved simple composition and are ubiquitously expressed in all animals that have ad eveloped immune response.T he natural production of antibodies that specifically recognize these "weak antigens" is difficult; [16] hence,traditional immunohistochemical methods for detecting glycosylations on cells are rare.A na lternative would be "plastic antibodies" or MIPs.…”

Molecularly Imprinted Biodegradable Nanoparticles

“…We have evaluate the affinity distribution of binding sites at different biotin concentration, following the method reported by Tse Sum Bui and Haupt25. Trends of bound ( B ) and free ( F ) biotin concentrations and the number of binding sites N for MIPs and CPs are summarized in 4a-b.…”

“…The affinity distribution was calculated with a graphical method, based on Freundlich isotherm, as reported in the literature25. Briefly, the trend of the concentration of bound ( B ) biotin as a function of free biotin ( F ) can be described with a power law:…”

Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging

“…[12][13][14][15] Consequences include effects on tumor growth, escape from apoptosis,m etastasis formation, and resistance to therapy.P olysaccharides involved in the glycosylation procedure have ah ighly conserved simple composition and are ubiquitously expressed in all animals that have ad eveloped immune response.T he natural production of antibodies that specifically recognize these "weak antigens" is difficult; [16] hence,traditional immunohistochemical methods for detecting glycosylations on cells are rare.A na lternative would be "plastic antibodies" or MIPs. [17,18] MIPs are tailormade synthetic antibody mimics that can recognize and bind target molecules specifically.They are synthesized by copolymerizing functional and cross-linking monomers in the presence of am olecular template,t hus resulting in the formation of binding sites with affinities and specificities comparable to those of natural antibodies.T heir molecularrecognition properties,c ombined with ah igh chemical and physical stability,m ake them interesting substitutes for antibodies in immunoassays, [19] biosensors, [20] bioseparation, [18,21] controlled drug release, [22] and bioimaging. [23][24][25] In this study,M IP-coated QDs were applied for the first time for the simultaneous multiplexed pseudoimmunolabeling and imaging of human keratinocytes.C ore-shell MIP nanoparticles for GlcA and NANA,( 125 AE 17) nm in size, were obtained, thus enabling the specific targeting of both intracellular and pericellular terminal glycosylations.W e previously reported 400 nm rhodamine-labeled MIP particles specific for GlcA that could only target the extracellular hyaluronan of the cell glycocalix.…”