Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging

Panagiotopoulou, Maria; Salinas, Yolanda; Beyazıt, Selim; Kunath, Stephanie; Duma, Luminita; Prost, Élise; Mayes, Andrew G.; Resmini, Marina; Bui, Bernadette Tse Sum; Haupt, Karsten

doi:10.1002/anie.201601122

Cited by 147 publications

(81 citation statements)

References 42 publications

(65 reference statements)

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“…A molar percentage ratio 2:5:8 of functional monomers comprising N ‐phenylacrylamide (PAA), 4‐acrylamidophenyl (amino)‐methaniminium acetate (AB, benzamidine‐based monomer), and N‐tert ‐butylacrylamide (TBAM), to enable π–π, electrostatic, and hydrophobic interactions, respectively, with the template, was used. N ‐isopropylacrylamide (NIPAM) functional monomer was added at a high molar ratio of minimum 80 % to endow the polymers with thermoresponsiveness and facilitate their elution from the solid support.…”

Section: Resultsmentioning

confidence: 99%

Chemical Antibody Mimics Inhibit Cadherin‐Mediated Cell–Cell Adhesion: A Promising Strategy for Cancer Therapy

et al. 2019

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One of the most promising strategies to treat cancer is the use of therapeutic antibodies that disrupt cell–cell adhesion mediated by dysregulated cadherins. The principal site where cell–cell adhesion occurs encompasses Trp2 found at the N‐terminal region of the protein. Herein, we employed the naturally exposed highly conserved peptide Asp1‐Trp2‐Val3‐Ile4‐Pro5‐Pro6‐Ile7, as epitope to prepare molecularly imprinted polymer nanoparticles (MIP‐NPs) to recognize cadherins. Since MIP‐NPs target the site responsible for adhesion, they were more potent than commercially available therapeutic antibodies for inhibiting cell–cell adhesion in cell aggregation assays, and for completely disrupting three‐dimensional tumor spheroids as well as inhibiting invasion of HeLa cells. These biocompatible supramolecular anti‐adhesives may potentially be used as immunotherapeutic or sensitizing agents to enhance antitumor effects of chemotherapy.

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Section: Resultsmentioning

confidence: 99%

Chemical Antibody Mimics Inhibit Cadherin‐Mediated Cell–Cell Adhesion: A Promising Strategy for Cancer Therapy

et al. 2019

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Section: Resultsmentioning

confidence: 99%

Chemical Antibody Mimics Inhibit Cadherin‐Mediated Cell–Cell Adhesion: A Promising Strategy for Cancer Therapy

et al. 2019

Self Cite

View full text Add to dashboard Cite

One of the most promising strategies to treat cancer is the use of therapeutic antibodies that disrupt cell-cell adhesion mediated by dysregulated cadherins.T he principal site where cell-cell adhesion occurs encompasses Trp2 found at the N-terminal region of the protein. Herein, we employed the naturally exposed highly conserved peptide Asp1-Trp2-Val3-Ile4-Pro5-Pro6-Ile7, as epitope to prepare molecularly imprinted polymer nanoparticles (MIP-NPs) to recognize cadherins.S ince MIP-NPs target the site responsible for adhesion, they were more potent than commercially available therapeutic antibodies for inhibiting cell-cell adhesion in cell aggregation assays,and for completely disrupting three-dimensional tumor spheroids as well as inhibiting invasion of HeLa cells.These biocompatible supramolecular anti-adhesives may potentially be used as immunotherapeutic or sensitizing agents to enhance antitumor effects of chemotherapy.

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“…This QD@MIPs not only had good specificity recognition (IF = 9.1), but also could detect 4‐nitrophenol through the change of fluorescence signal . Also using QDs as fluorescence signal, Panagiotopoulou et al prepared fluorescent core‐shell particles that specifically recognized N ‐acetylneuraminic acid or glucuronic acid and used them in cell imaging.…”

Section: The Currently Popular Imprinting Materialsmentioning

confidence: 99%

Molecularly Imprinted Materials for Selective Biological Recognition

Zhang

et al. 2019

Macromol. Rapid Commun.

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Molecular imprinting is an approach of generating imprinting cavities in polymer structures that are compatible with the target molecules. The cavities have memory for shape and chemical recognition, similar to the recognition mechanism of antigen–antibody in organisms. Their structures are also called biomimetic receptors or synthetic receptors. Owing to the excellent selectivity and unique structural predictability of molecularly imprinted materials (MIMs), practical MIMs have become a rapidly evolving research area providing key factors for understanding separation, recognition, and regenerative properties toward biological small molecules to biomacromolecules, even cell and microorganism. In this review, the characteristics, morphologies, and applicability of currently popular carrier materials for molecular imprinting, especially the fundamental role of hydrogels, porous materials, hierarchical nanoparticles, and 2D materials in the separation and recognition of biological templates are discussed. Moreover, through a series of case studies, emphasis is given on introducing imprinting strategies for biological templates with different molecular scales. In particular, the differences and connections between small molecular imprinting (bulk imprinting, “dummy” template imprinting, etc.), large molecular imprinting (surface imprinting, interfacial imprinting, etc.), and cell imprinting strategies are demonstrated in detail. Finally, future research directions are provided.

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Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging

Cited by 147 publications

References 42 publications

Chemical Antibody Mimics Inhibit Cadherin‐Mediated Cell–Cell Adhesion: A Promising Strategy for Cancer Therapy

Chemical Antibody Mimics Inhibit Cadherin‐Mediated Cell–Cell Adhesion: A Promising Strategy for Cancer Therapy

Chemical Antibody Mimics Inhibit Cadherin‐Mediated Cell–Cell Adhesion: A Promising Strategy for Cancer Therapy

Molecularly Imprinted Materials for Selective Biological Recognition

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