Preparation and evaluation in vitro and in vivo of fibrinogen microspheres containing adriamycin.

Miyazaki, Shôzo; Hashiguchi, Norio; Hou, Weimin; Yokouchi, Chizuko; Takada, Masahiko

doi:10.1248/cpb.34.3384

Cited by 29 publications

(9 citation statements)

References 1 publication

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“…21 A designated amount of chitosan was dissolved in 10 mL of 5% (v/v) acetic acid aqueous solution and filtered to remove any undissolved particulate. 21 A designated amount of chitosan was dissolved in 10 mL of 5% (v/v) acetic acid aqueous solution and filtered to remove any undissolved particulate.…”

Section: Methodsmentioning

confidence: 99%

Optimization of the Formulation Design of Chitosan Microspheres Containing Cisplatin

Wang¹,

Sato

Adachi

et al. 1996

Journal of Pharmaceutical Sciences

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This study describes an orthogonal experimental design to optimize the formulation of cisplatin (CDDP)-loaded chitosan microspheres (namely, CDDP-DAC-MS) which were produced by an emulsion-chemical cross-linking technique. Seven factors and three levels for each factor that might affect the formulation of microspheres were selected and arranged in an L27(3(13)) orthogonal experimental table. A desirability function (df) calculated according to the trapping efficiency of CDDP, the drug content (%, w/w), and the size distribution of each batch of microspheres was introduced as an index of the microsphere formulation. The overall desirability functions (DF) were produced and treated by a statistic analytical system to optimize the formulation. Moreover, the contour maps were produced to analyze the influence of the seven factors on the size distribution, the drug content, and the drug trapping efficiency. The established optimum procedure was reproducible. Scanning electron micrographs showed that CDDP-DAC-MS were spherical with a coarse surface. The average diameter, drug content, and drug trapping efficiency of CDDP-DAC-MS were 74.8 microns, 20.8% (w/w), and 77.5%, respectively. The in vitro release of cisplatin from chitosan microspheres in saline was retarded compared with that from saline solution; the release of CDDP from chitosan microspheres was suggested to be controlled by the dissolution and diffusion of the drug from the chitosan matrix.

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Section: Methodsmentioning

confidence: 99%

Optimization of the Formulation Design of Chitosan Microspheres Containing Cisplatin

Wang¹,

Sato

Adachi

et al. 1996

Journal of Pharmaceutical Sciences

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“…In one study, water soluble, 5-fluorouracil-loaded Fbg microspheres were effectively used as a novel injectable drug delivery system for anticancer treatment and expressed sustained release for more than 5 days, as demonstrated by in vitro experiment. 39 The same research group demonstrated doxorubicin release in mice, and positive results were observed on the basis of animal survival data. The doxorubicin solution-injected mice showed decreased lifespan, with 50% dead at 10 days and no survivors after 28 days, whereas doxorubicin-loaded-Fbg-microsphereinjected mice showed significantly increased lifespan, and 50% mortality occurred after 60 days.…”

Section: Cancer Therapymentioning

confidence: 96%

Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications

Rajangam¹,

An²

2013

IJN

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Over the past two decades, many types of natural and synthetic polymer-based micro-and nanocarriers, with exciting properties and applications, have been developed for application in various types of tissue regeneration, including bone, cartilage, nerve, blood vessels, and skin. The development of suitable polymers scaffold designs to aid the repair of specific cell types have created diverse and important potentials in tissue restoration. Fibrinogen (Fbg)-and fibrin (Fbn)-based micro-and nanostructures can provide suitable natural matrix environments. Since these primary materials are abundantly available in blood as the main coagulation proteins, they can easily interact with damaged tissues and cells through native biochemical interactions. Fbg-and Fbn-based micro and nanostructures can also be consecutively furnished/ or encapsulated and specifically delivered, with multiple growth factors, proteins, and stem cells, in structures designed to aid in specific phases of the tissue regeneration process. The present review has been carried out to demonstrate the progress made with micro and nanoscaffold applications and features a number of applications of Fbg-and Fbn-based carriers in the field of biomaterials, including the delivery of drugs, active biomolecules, cells, and genes, that have been effectively used in tissue engineering and regenerative medicine.

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“…For the incorporation of drugs into Fbg microspheres, the drug solution can be added to the W/O emulsion before adding an emulsifier. Using this method, some anti-cancer drugs, such as 5-fluorouracil 38 and adriamycin 39,40 , have been encapsulated, suggesting further applications in other drug incorporation studies. In our previous study, Fbg microspheres were similarly developed using the W/O emulsification method, with the exception of the solvent being evaporated by adding acetone instead of the use of pre-heated oil in order to decrease protein denaturation 17 .…”

Section: Fabrications Of Fbg Drug Carriersmentioning

confidence: 99%

Fibrinogen as a promising material for various biomedical applications

Joo

Amin

Rajangam

et al. 2015

Mol. Cell. Toxicol.

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As demands for innovative drug carriers and transplantable organs increase, many researchers have developed diverse drug carriers and scaffolds using various materials. However, several candidate materials have shown systemic toxicity, making them unsuitable for clinical use. Fibrinogen (Fbg), a natural polymer, could be a promising material for applications in biomedical engineering owing to its biocompatibility and biodegradability, as reported in numerous studies. Moreover, autologous Fbg is abundant in blood and can be easily extracted, presenting Fbg as an excellent biomaterial for biomedical applications due to minimal immunological rejection. In addition, the biocompatibility of other materials could be improved by combining them with Fbg. Over the next few years, Fbg could be widely used in various biomedical and clinical fields. Here, we discuss the characteristics of Fbg and fabricating methods for Fbg scaffolds in various biomedical applications. The future prospects of Fbg as an applicable biomaterial, especially in organ fabrication by the cutting-edge 3D bioprinting technology, are also presented.

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Preparation and evaluation in vitro and in vivo of fibrinogen microspheres containing adriamycin.

Cited by 29 publications

References 1 publication

Optimization of the Formulation Design of Chitosan Microspheres Containing Cisplatin

Optimization of the Formulation Design of Chitosan Microspheres Containing Cisplatin

Fibrinogen and fibrin based micro and nano scaffolds incorporated with drugs, proteins, cells and genes for therapeutic biomedical applications

Fibrinogen as a promising material for various biomedical applications

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