Preparation and Chemical Properties of Colicine I

Keene, John H.

doi:10.1139/m66-061

Cited by 11 publications

(4 citation statements)

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“…Different from these bacteriocins are those which have been identified as lipo-polysaccharide-protein complexes. Colicin V-K 357 (22) and colicin I, produced by Salmonella strasbourg (24), and a bacteriocin produced by Lactobacillus fermenti strain 466 (26) have been shown to be lipo-polysaccharide-protein complexes. Staphylococcin 1580 seems to belong to this class of bacteriocins, whereas the bacteriocin produced by Staphylococcus aureus type 71, isolated by Dajani et al (5), is only proteinaceous of nature.…”

Section: Discussionmentioning

confidence: 99%

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Production and Purification of a Staphylococcus epidermidis Bacteriocin

1972

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Liquid cultures of Staphylococcus epidermidis 1580 contained rather small amounts of a bacteriocin, staphylococcin 1580, which was found both in the supernatant fluid and in the cell pellet. It could be extracted from the cells with 5% NaCl solution. The staphylococcin production could not be induced by ultraviolet irradiation or treatment with mitomycin C. Bacteria grown on semisolid medium produced a much larger amount of the compound with a high specific activity. The staphylococcin was purified by ammonium sulfate precipitation, ultracentrifugation, and chromatography on Sephadex columns. The purified material was homogeneous on polyacrylamide gel electrophoresis. The molecular weight was between 150,000 and 400,000. The bacteriocin was composed of protein, carbohydrate, and lipid and consisted of subunits exhibiting a molecular weight of about 20,000.

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Section: Discussionmentioning

confidence: 99%

“…Investigations of the chemical nature of bacteriocins have shown them to be a heterogeneous group of substances ranging from simple proteins (7,13,17,20) and proteins complexed with carbohydrates and lipids (22,24,26) to particles resembling phages (18,25,30,40).…”

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Production and Purification of a Staphylococcus epidermidis Bacteriocin

1972

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“…fu eron incubados a la te mperatu con ag itación constante de 60 pul hasta alcanzar la fase estacionaria E xtracción de la substancia Los sobrenadantes colectados f tidos a precipitación fraccionada con s ulfato de amonio (Merck) ha 70% de saturación (Keen, 1966). 14-16 h de mantenerlas a 4 oC, l se centrifugaron a 5 000 x g por precipitado fue di suelto en agua d téril.…”

Section: Materials Y Métodosunclassified

Caracterización parcial y espectro antimicrobiano de substancias inhibitorias producidas por Alteromonas marinas

León

Tapia

1999

Rev peru biol

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RESUMENSe caracterizan parcialmente substancias inhibitorias produci das por cepas marinas del gé Alteromonas spp. Pruebas de precipitación de extractos crudos de sobrenadantes de las cepas N22 N11 .6 con concentraciones saturantes de sulfato de amonio y fi ltrados en columnas de SephadexG muestran la producción de substancias inhibitorias (SI) de naturaleza proteinácea.Otros ensayos de caracterización revelan que dichas substancias tienen un amplio espectro de vidad antibiótica frente a cepas de colecci ón patógenas de peces , moluscos y crustáceos . Asimis muestran a las SI como termosensibles a 90 O c en 45 min y con capacidad de mantener estabil inhibitoria dentro del rango de pH de 3,0 a 9,0 hasta por 15 mino La actividad antibiótica de las subs cias frente a Vibrio anguillarum NCMB 2133, V. angufflarum ATCC 19264 y Aeromonas salmon 67,79 fueron comparativamente equivalentes a 30 ppm del antibiótico oxitetraciclina (OTC) .Palabras claves: bacterias marinas, actividad inhibitoria, substancia extracelular, Alteromo antibiosis . ABSTRACTInhibitory substances produced by marine strains belonglng to the genus Alteromonas spp. partially characte rized . Precipitaban of raw extracts supernatans from strains N22.C and N11 .6 concentration saturants of ammonium sulfate and fi ltrates in columns Sephadex G-25 show production of inhibitory substances (SI) of proteinacea nature . Other assays revealed that th substances have a wide spectrum of antibiotic activity against collection strains pathogens of mollusks and crustaceans. Also, it shows those SI to be thermosensitive at 90 ºC for 45 min and s within the pH range of 3,0 at 9,0 for 15 min o Antibiotic activity ofthe substances against Vibrio anguilla NCMB 2133, V anguillarum ATCC 19264 and A eromonas salmonicida 76,79 was comparat equivalent to 30 ppm of the antibiotic oxitetraciclina (OTC) .

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“…The colicins, bactericidal agents produced by enterobacteria, can be induced in bacterial strains possessing an episome called the "colicinogenic factor." Early reports in the literature concerning their composition made mention of proteo-lipopolysaccharide complexes (2,11,14,15) as being the native colicin; some years later, evidence was presented showing that the protein moiety is responsible for the lethal activity and can be isolated in pure form (5, 12; M. A. Jesaitis, Bacteriol. Proc., p. 50, 1967).…”

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Chemical and Immunological Study of Colicins E ₁ , K, A, and Q

Dandeu

1971

Infect Immun

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Colicins are proteins and, regardless of their origin, can exist in one of two forms, free or bound. When bound, the other constituent is a polysaccharide, but the ratio of the bound form to the free form is dependent on whether the colicin is extracted from an induced or a noninduced strain. Even after extensive purification, traces of polysaccharides persist, and this may be responsible for the partial resistance of colicins to denaturation. The different colicins examined showed differences in their electrophoretic mobility as well as in their amino acid content. However, similarities do exist as has been shown by the demonstration of a common antigen site "L" to colicins El, Q, and H.

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Preparation and Chemical Properties of Colicine I

Cited by 11 publications

References 0 publications

Production and Purification of a Staphylococcus epidermidis Bacteriocin

Production and Purification of a Staphylococcus epidermidis Bacteriocin

Caracterización parcial y espectro antimicrobiano de substancias inhibitorias producidas por Alteromonas marinas

Chemical and Immunological Study of Colicins E ₁ , K, A, and Q

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Preparation and Chemical Properties of Colicine I

Cited by 11 publications

References 0 publications

Production and Purification of a Staphylococcus epidermidis Bacteriocin

Production and Purification of a Staphylococcus epidermidis Bacteriocin

Caracterización parcial y espectro antimicrobiano de substancias inhibitorias producidas por Alteromonas marinas

Chemical and Immunological Study of Colicins E 1 , K, A, and Q

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Chemical and Immunological Study of Colicins E ₁ , K, A, and Q