Preparation and Characterization of Trypsin Immobilized on Tygon Tubing

Immobilization of Enzymes on Flexible Tubing Surfaces for Continuous Bioassays

“…Alternatively, enzymes can be immobilized inside a hollow millifluidic tubing with a large surface-to-volume ratio to increase the apparent enzyme activity. In the literature, immobilization of enzymes inside millifluidic tubing for continuous, enzyme-catalyzed reactions has been reported. − Moreover, immobilization of enzymes on different types of tubing materials such as Tygon and polytetrafluoroethylene (PTFE) has also been investigated. Tygon tubing is highly flexible, biocompatible, and suitable for handling corrosive liquids and gases.…”

“…It has smooth surface finishing and a low coefficient of friction, which facilitates drainage of corrosive liquids. As a result, many researchers developed different enzyme immobilization methods on Tygon tubing for various applications. ,,,− For example, enzymes can be directly immobilized on Tygon tubing by physical adsorption . Although the immobilized enzymes retain high enzymatic activity, this mechanism causes nonspecific adsorption of enzyme and has poor stability.…”

Of Meat, Men and Property: The Troubled Career of a Convert Nun in Eighteenth-Century Kiev

“…Sophia Senyk describes the absence of common life as 'one of the ills of eastern monasticism, in all countries and at all 4 periods'. 7 Possession of money, goods and chattels by monks and nuns contradicted the most basic principles of monastic life such as the rule of poverty and obedience to superiors, complicated hierarchical relationships, created the sense of inequality among the inmates, and distracted them from the daily regimen of prayer and contemplation. 8 The only two areas that remained common to the inmates within the ideorrythmicallyorganised monastic community, were liturgic services and the cemetery.…”