Immobilization of Enzymes on Flexible Tubing Surfaces for Continuous Bioassays

Jannat, Mahbuba; Yang, Kun‐Lin

doi:10.1021/acs.langmuir.8b02991

Cited by 10 publications

(11 citation statements)

References 54 publications

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“…The enzyme was loaded though electrostatic interaction with minimum influence on the enzyme configuration. At the same time, blocking of the active sites after enzyme loading in a confined environment would reduce the enzyme activity [ 44 , 45 , 46 ]. Catalase loaded in HMSN-1.1 has the best-preserved enzyme activity (63%), suggesting that catalase loaded in HMSN-1.1 has more accessible active sites.…”

Section: Resultsmentioning

confidence: 99%

Dendritic Mesoporous Silica Hollow Spheres for Nano-Bioreactor Application

Zhang

Fang

et al. 2022

Nanomaterials

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Mesoporous silica materials have attracted great research interest for various applications ranging from (bio)catalysis and sensing to drug delivery. It remains challenging to prepare hollow mesoporous silica nanoparticles (HMSN) with large center-radial mesopores that could provide a more efficient transport channel through the cell for guest molecules. Here, we propose a novel strategy for the preparation of HMSN with large dendritic mesopores to achieve higher enzyme loading capacity and more efficient bioreactors. The materials were prepared by combining barium sulfate nanoparticles (BaSO4 NP) as a hard template and the in situ-formed 3-aminophenol/formaldehyde resin as a porogen for directing the dendritic mesopores’ formation. HMSNs with different particle sizes, shell thicknesses, and pore structures have been prepared by choosing BaSO4 NP of various sizes and adjusting the amount of tetraethyl orthosilicate added in synthesis. The obtained HMSN-1.1 possesses a high pore volume (1.07 cm3 g−1), a large average pore size (10.9 nm), and dendritic mesopores that penetrated through the shell. The advantages of HMSNs are also demonstrated for enzyme (catalase) immobilization and subsequent use of catalase-loaded HMSNs as bioreactors for catalyzing the H2O2 degradation reaction. The hollow and dendritic mesoporous shell features of HMSNs provide abundant tunnels for molecular transport and more accessible surfaces for molecular adsorption, showing great promise in developing efficient nanoreactors and drug delivery vehicles.

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Section: Resultsmentioning

confidence: 99%

Dendritic Mesoporous Silica Hollow Spheres for Nano-Bioreactor Application

Zhang

Fang

et al. 2022

Nanomaterials

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“…An attempt to directly correlate the research results with literature data is quite difficult. This is due to the incompatibility of catalytic structures with other synzymes with peptidic active fragment [44,45,46,47,48,49,50]. The comparison of the effectiveness of N -lipidated peptides immobilized on cellulose to catalyse the hydrolysis of esters [40,41] and amides indicates that degradation of amide/peptide bond is more demanding and possible only with highly active catalytic systems.…”

Section: Resultsmentioning

confidence: 99%

N-Lipidated Amino Acids and Peptides Immobilized on Cellulose Able to Split Amide Bonds

Frączyk

Kamiński

2019

Materials

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N-lipidated short peptides and amino acids immobilized on the cellulose were used ascatalysts cleaved amide bonds under biomimetic conditions. In order to select catalytically mostactive derivatives a library of 156 N-lipidated amino acids, dipeptides and tripeptides immobilizedon cellulose was obtained. The library was synthesized from serine, histidine and glutamic acidpeptides N-acylated with heptanoic, octanoic, hexadecanoic and (E)-octadec-9-enoic acids.Catalytic efficiency was monitored by spectrophotometric determination of p-nitroaniline formedby the hydrolysis of a 0.1 M solution of Z-Leu-NP. The most active 8 structures contained tripeptidefragment with 1-3 serine residues. It has been found that incorporation of metal ions into catalyticpockets increase the activity of the synzymes. The structures of the 17 most active catalysts selectedfrom the library of complexes obtained with Cu2+ ion varied from 16 derivatives complexed withZn2+ ion. For all of them, a very high reaction rate during the preliminary phase of measurementswas followed by a substantial slowdown after 1 h. The catalytic activity gradually diminished aftersubsequent re-use. HPLC analysis of amide bond splitting confirmed that substrate consumptionproceeded in two stages. In the preliminary stage 24–40% of the substrate was rapidly hydrolysedfollowed by the substantially lower reaction rate. Nevertheless, using the most competentsynzymes product of hydrolysis was formed with a yield of 60–83% after 48h under mild andstrictly biomimetic conditions.

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“…This technique combines purification and stabilisation into a single step, and can be performed directly on crude enzyme extracts, such as those obtained from AS [155]. Furthermore, immobilising enzymes by cross-linking to the surface of a membrane or micro-reactor for use within a continuous reaction process [156] offers significant potential opportunities and is an exciting area for future research and development. For instance, Barber et al [157] recently proposed that oxidoreductase enzymes cross-linked with flexible spacers (e.g.…”

Section: Stabilisation/immobilisationmentioning

confidence: 99%

Enzyme Recovery from Biological Wastewater Treatment

Liu¹,

Smith²

2020

Waste Biomass Valor

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Enzymes are high value industrial bio-catalysts with extensive applications in a wide range of manufacturing and processing sectors, including the agricultural, food and household care industries. The catalytic efficiency of enzymes can be several orders higher compared to inorganic chemical catalysts under mild conditions. However, the nutrient medium necessary for biomass culture represents a significant cost to industrial enzyme production. Activated sludge (AS) is a waste product of biological wastewater treatment and consists of microbial biomass that degrades organic matter by producing substantial quantities of hydrolytic enzymes. Therefore, enzyme recovery from AS offers an alternative, potentially viable approach to industrial enzyme production. Enzyme extraction from disrupted AS flocs is technically feasible and has been demonstrated at experimental-scale. A critical review of disruption techniques identified sonication as potentially the most effective and suitable method for enzyme extraction, which can be scaled up and is a familiar technology to the water industry. The yields of different enzymes are influenced by wastewater treatment conditions, and particularly the composition, and can also be controlled by feeding sludge with specific target substrates. Nevertheless, hydrolytic enzymes can be effectively extracted directly from waste AS without specific modifications to standard wastewater treatment processes. Purification, concentration and stabilisation/immobilisation techniques can also greatly expand the industrial application and increase the economic value and marketability of enzyme products extracted from AS. Concentrated and purified AS enzymes could readily substitute inorganic and/or commercial bioenzyme catalysts in many industrial applications including, for example, leather processing, and in detergent and animal feed formulation. Enzyme extraction from AS therefore offers significant economic benefits to the Water Industry by recovering valuable resources from wastewater. They can also optimise important waste treatment processes, such as the anaerobic digestion (AD) of sewage sludge, increasing biogas and renewable energy production. The enzyme-extracted sludge exhibits improved treatment properties, such as increased settleability, dewaterability, and anaerobic digestibility for biogas production, assisting sludge management by wastewater treatment plants (WWTPs) and enabling the further utilisation of the residual sludge. Graphic Abstract

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Immobilization of Enzymes on Flexible Tubing Surfaces for Continuous Bioassays

Cited by 10 publications

References 54 publications

Dendritic Mesoporous Silica Hollow Spheres for Nano-Bioreactor Application

Dendritic Mesoporous Silica Hollow Spheres for Nano-Bioreactor Application

N-Lipidated Amino Acids and Peptides Immobilized on Cellulose Able to Split Amide Bonds

Enzyme Recovery from Biological Wastewater Treatment

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