Preparation and characterization of spray-dried gelatin microspheres encapsulating ganciclovir

Tran, Tuan Hiep; Ramasamy, Thiruganesh; Poudel, Bijay Kumar; Marasini, Nirmal; Moon, Bo Kyung; Cho, Hyuk Jun; Choi, Han‐Gon; Yong, Chul Soon; Kim, Jong Oh

doi:10.1007/s13233-014-2018-9

Cited by 21 publications

(15 citation statements)

References 26 publications

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“…These changes could be due to the increased water loss in beads added with gelatin during the drying process, as well as the fact that gelatin is a highly aqueous soluble polymer that dissolves rapidly in an aqueous environment. [20] In addition, SEM micrographs revealed the structure of microparticles. The drying process altered the shape of the beads, and the shape was dependent on the concentration of additives.…”

Section: Microscopic Examination Of Microcapsulesmentioning

confidence: 99%

Preparation and characterization of alginate–gelatin microencapsulatedBacillus subtilisSL-13 by emulsification/internal gelation

Liang

Yang

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Gelatin was blended with sodium alginate (NaALG) to obtain a novel microbial fungicide, and dispersed micron Bacillus subtilis SL-13 microspheres prepared by emulsification/internal gelation method. Microscopic examination revealed that microcapsules were nearly spherical in shape. Fourier transform infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction confirmed that the electrostatic interaction was occurred when gelatin added into NaALG. The maximum encapsulation efficiency was 93.44% at a gelatin concentration of 1.5%. Particle size, swelling, and biodegradation of beads increased with gelatin content increase. Furthermore, the viability of encapsulated SL-13 could be preserved at more than 10(8) CFU/mL after 120 d storage at 25 °C. The number of viable cells released from microcapsules presented an initial rapid increase followed by a gradual increase, and reached the maximum as 10(10) CFU/mL on day 35. Thus, it is feasible to prepare uniform, rounded shape, and well-dispersed micron microcapsules of SL-13 via emulsification/internal gelation using NaALG and gelatin composites. This encapsulation strategy could be considered as a potential alternative to future applications in the agricultural industry.

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Section: Microscopic Examination Of Microcapsulesmentioning

confidence: 99%

Preparation and characterization of alginate–gelatin microencapsulatedBacillus subtilisSL-13 by emulsification/internal gelation

Liang

Yang

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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“…The samples collected after spray-drying presented a mixture of wrinkled (mostly) and a few smooth spherical particles, as depicted in figure 3, following a unimodal distribution ( figure 4), in accordance with the lower values (table 2). Irregular particle morphology was already reported for gelatin microspheres [19], and is usually…”

Section: Optimization Of Gelatin Microparticlesmentioning

confidence: 61%

“…Ciprofloxacin (CPx)-loaded gelatin microparticles were produced by single-step spraydrying following the best experimental conditions determined in section 3.1. The gel crosslinking was performed in the vapor phase to improve particle stability, while diminishing possible cytotoxic effects associated with the crosslinking agent (GA) [19,40]. Both uncross and crosslinked particles obtained by spray-drying presented a wrinkled surface morphology (figure 5 a -d) like the ones observed in the orthogonal experiments ( figure 3).…”

Section: Characterization Of Gel-cpx Particlesmentioning

confidence: 92%

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Development and optimization of ciprofloxacin-loaded gelatin microparticles by single-step spray-drying technique

et al. 2018

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Polymeric particles are a versatile class of local or systemic drug delivery systems, used to improve drugs pharmacokinetics and pharmacodynamics along with patient compliance. Herein, we report a rapid, scalable, and optimized method to encapsulate ciprofloxacin (CP x ), a poor water soluble antimicrobial agent, in gelatin microparticles by single step processing via spray-drying of an aqueous solution. The developed particles show mainly a wrinkle morphology with a unimodal distribution, with mean diameters ranging between 2 and 4 μm, depending on the processing conditions. The encapsulation of 1, 2 and 5 wt% CPx narrows the size distribution (1-3 μm). In vitro release experiments showed that up to 80% of encapsulated drug is released during the first 6 h, and the release kinetics was best fitted with the Korsmeyer-Peppas model, explained by the superimposition of drug diffusion and polymer chain relaxation. The minimal inhibitory concentrations against S. aureus and E. coli, obtained from pure and encapsulated ciprofloxacin, demonstrated that the spray-drying process does not inhibit the drug's bioactivity or the process feasibility. Thus, spray-drying of protein-drug particle systems is an advantageous method to produce microparticles with potential to lung delivery systems. Smith, M. L. & Sencadas, V. (2018). Development and optimization of ciprofloxacin-loaded gelatin microparticles by single-step spray-drying technique. Powder Technology, 330 201-209. Abstract:Polymeric particles are a versatile class of local or systemic drug delivery systems, used to improve drugs pharmacokinetics and pharmacodynamics along with patient compliance. Herein, we report a rapid, scalable, and optimized method to encapsulate ciprofloxacin (CPx), a poor water soluble antimicrobial agent, in gelatin microparticles by single step processing via spray-drying of an aqueous solution. The developed particles show mainly a wrinkle morphology with a unimodal distribution, with mean diameters ranging between 2 and 4 , depending on the processing conditions. The encapsulation of 1, 2 and 5 wt% CPx narrows the size distribution (1-3 m). In vitro release experiments showed that up to 80% of encapsulated drug is released during the first 6 hours, and the release kinetics was best fitted with the Korsmeyer-Peppas model, explained by the superimposition of drug diffusion and polymer chain relaxation. The minimal inhibitory concentrations against S. aureus and E. coli, obtained from pure and encapsulated ciprofloxacin, demonstrated that the spraydrying process does not inhibit the drug`s bioactivity or the process feasibility. Thus, spray-drying of protein-drug particle systems is an advantageous method to produce microparticles with potential to lung delivery systems.

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“…Because the form is isotropic, microspheres can be easily prepared under a fluid or gas atmosphere. Several methods have been developed to prepare the microspheres, which include water‐in‐oil emulsion, water‐in‐oil‐in‐water emulsion, electrospinning, microsphere gelation, spray drying, suspension polymerization, ultrasonication and phase separation . Among others, the water‐in‐oil emulsion is generally used to prepare microspheres for the culture of cells, where a hydrophilic solution is stirred in a continuous hydrophobic phase to from spherical droplets which then separated from the hydrophobic phase.…”

Section: Biomaterials Used For Liver Regeneration: a Brief Summarymentioning

confidence: 99%

Biomaterials and Culture Technologies for Regenerative Therapy of Liver Tissue

Pérez

Jung

Kim

2016

Adv Healthcare Materials

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Regenerative approach has emerged to substitute the current extracorporeal technologies for the treatment of diseased and damaged liver tissue. This is based on the use of biomaterials that modulate the responses of hepatic cells through the unique matrix properties tuned to recapitulate regenerative functions. Cells in liver preserve their phenotype or differentiate through the interactions with extracellular matrix molecules. Therefore, the intrinsic properties of the engineered biomaterials, such as stiffness and surface topography, need to be tailored to induce appropriate cellular functions. The matrix physical stimuli can be combined with biochemical cues, such as immobilized functional groups or the delivered actions of signaling molecules. Furthermore, the external modulation of cells, through cocultures with nonparenchymal cells (e.g., endothelial cells) that can signal bioactive molecules, is another promising avenue to regenerate liver tissue. This review disseminates the recent approaches of regenerating liver tissue, with a focus on the development of biomaterials and the related culture technologies.

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Preparation and characterization of spray-dried gelatin microspheres encapsulating ganciclovir

Cited by 21 publications

References 26 publications

Preparation and characterization of alginate–gelatin microencapsulatedBacillus subtilisSL-13 by emulsification/internal gelation

Preparation and characterization of alginate–gelatin microencapsulatedBacillus subtilisSL-13 by emulsification/internal gelation

Development and optimization of ciprofloxacin-loaded gelatin microparticles by single-step spray-drying technique

Biomaterials and Culture Technologies for Regenerative Therapy of Liver Tissue

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