Preparation and characterization of alginate–gelatin microencapsulatedBacillus subtilisSL-13 by emulsification/internal gelation

Abstract

Gelatin was blended with sodium alginate (NaALG) to obtain a novel microbial fungicide, and dispersed micron Bacillus subtilis SL-13 microspheres prepared by emulsification/internal gelation method. Microscopic examination revealed that microcapsules were nearly spherical in shape. Fourier transform infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction confirmed that the electrostatic interaction was occurred when gelatin added into NaALG. The maximum encapsulation efficiency was 93.44% at a gelatin concentration of 1.5%. Particle size, swelling, and biodegradation of beads increased with gelatin content increase. Furthermore, the viability of encapsulated SL-13 could be preserved at more than 10(8) CFU/mL after 120 d storage at 25 °C. The number of viable cells released from microcapsules presented an initial rapid increase followed by a gradual increase, and reached the maximum as 10(10) CFU/mL on day 35. Thus, it is feasible to prepare uniform, rounded shape, and well-dispersed micron microcapsules of SL-13 via emulsification/internal gelation using NaALG and gelatin composites. This encapsulation strategy could be considered as a potential alternative to future applications in the agricultural industry.

Hybrid cellulose nanocrystal/alginate/gelatin scaffold with improved mechanical properties and guided wound healing

“…Besides, SA : Ge ¼ 2 : 1 group also possessed a moderate swelling degree which could provide a relative humid environment for wounds without excessive dehydration. [32][33][34] Thus, from the above results, the ratio of SA : Ge ¼ 2 : 1 was selected for further study with CNC to deeply improve its properties.…”

3D Encapsulation Made Easy: A Coaxial-Flow Circuit for the Fabrication of Hydrogel Microfibers Patches

“…Contrarily, HA did not have the same effect at both of the concentrations chosen for blending (0.1% and 0.2% ( w / v ), Figure 3c) [40,41]. Due to the high molecular weight, HA was added in at lower percentages, and the increase in viscosity at room temperature was less pronounced (Figure 4a).…”

Polycaprolactone microcapsules containing citric acid and naringin for plant growth and sustainable agriculture: physico-chemical properties and release behavior

“…This is accomplished by a physical-chemical characterization of the MCs to evaluate the effects of pH and RE on the encapsulated molecules release kinetics. Finally, it should be stressed that not many articles where the encapsulation of sustainable biofertilizers is reported, are found in the literature (Li et al, 2017;Tu et al, 2016;Tu et al, 2015;Wu et al, 2014). Microencapsulation of bacterial cells (bacterial fertilizers) was reported using a mixture of sodium alginate and maltodextrin or polybutylene succinate and starch as a cover material (Campos et al, 2014;Wu, 2008); plant growth regulators, such as a-naphthalene acetate, were loaded into double-layered inorganic matrices (Hussein et al, 2002); bioflavonoids, such as hesperidin, were encapsulated within alginate microparticles (Tsirigotis-Maniecka et al, 2017).…”