Preparation and characterization of silver nanoparticle loaded amorphous hydrogel of carboxymethylcellulose for infected wounds

Das, Anup Kumar; Kumar, Ajay; Patil, Niranjan B.; Viswanathan, Chandra; Ghosh, Deepa

doi:10.1016/j.carbpol.2015.03.082

Cited by 81 publications

(41 citation statements)

References 29 publications

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“…Several authors reported the preparation of topically applied formulations intended for the delivery of AgNPs. 21,24,29,30 The present work aimed at biological synthesis of AgNPs in a simple, ecofriendly, and cost-effective manner using fungus Fusarium verticillioides ASU1 (KT587649). A comparative detailed study based on the effect of three factors on AgNPs activity was provided.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels

Mekkawy

El‐Mokhtar²,

Nafady

et al. 2017

IJN

152

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In the present study, silver nanoparticles (AgNPs) were synthesized via biological reduction of silver nitrate using extract of the fungus Fusarium verticillioides (green chemistry principle). The synthesized nanoparticles were spherical and homogenous in size. AgNPs were coated with polyethylene glycol (PEG) 6000, sodium dodecyl sulfate (SDS), and β-cyclodextrin (β-CD). The averaged diameters of AgNPs were 19.2±3.6, 13±4, 14±4.4, and 15.7±4.8 nm, for PEG-, SDS-, and β-CD-coated and uncoated AgNPs, respectively. PEG-coated AgNPs showed greater stability as indicated by a decreased sedimentation rate of particles in their water dispersions. The antibacterial activities of different AgNPs dispersions were investigated against Gram-positive bacteria (methicillin-sensitive and methicillin-resistant Staphylococcus aureus ) and Gram-negative bacteria ( Escherichia coli ) by determination of the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). MIC and MBC values were in the range of 0.93–7.5 and 3.75–15 µg/mL, respectively, which were superior to the reported values in literature. AgNPs-loaded hydrogels were prepared from the coated-AgNPs dispersions using several gelling agents (sodium carboxymethyl cellulose [Na CMC], sodium alginate, hydroxypropylmethyl cellulose, Pluronic F-127, and chitosan). The prepared formulations were evaluated for their viscosity, spreadability, in vitro drug release, and antibacterial activity, and the combined effect of the type of surface coating and the polymers utilized to form the gel was studied. The in vivo wound-healing activity and antibacterial efficacy of Na CMC hydrogel loaded with PEG-coated AgNPs in comparison to the commercially available silver sulfadiazine cream (Dermazin ® ) were evaluated. Superior antibacterial activity and wound-healing capability, with normal skin appearance and hair growth, were demonstrated for the hydrogel formulations, as compared to the silver sulfadiazine cream. Histological examination of the treated skin was performed using light microscopy, whereas the location of AgNPs in the skin epidermal layers was visualized using transmission electron microscopy.

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Section: Introductionmentioning

confidence: 99%

In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels

Mekkawy

El‐Mokhtar²,

Nafady

et al. 2017

IJN

152

View full text Add to dashboard Cite

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“…0.1% (w/w) polyethylene glycol (PEG) protected SNP incorporated into PCL scaffold was found to be effective as an antimicrobial agent due to the presence of silver ions, which is the active ingredient . Potential of SNP‐loaded hydrogels has also been extensively investigated . In this study, we co‐polymerized GelMA with an alternate monomer 2‐hydroxypropyl methacrylate (HPMA) in the presence of silver nanoparticles using redox polymerization technique and the resultant antimicrobial hydrogel was expected to possess excellent mechanical stability and optimum water hydration properties suitable for wound dressings.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of silver nanoparticle incorporated gelatin‐hydroxypropyl methacrylate hydrogels for wound dressing applications

Resmi

Unnikrishnan

Krishnan

et al. 2016

J of Applied Polymer Sci

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A replaceable wound cover which absorbs moisture and resist infection can be used to prevent development of chronic wounds. A major criterion for a replaceable wound dressing is nonadherence to cells to prevent pain upon removal. A major limitation of water absorbing hydrogels used in wound dressing applications is their poor mechanical strength. In this study, gelatin methacrylate (GelMA) was synthesized by reacting Type A porcine skin gelatin with methacrylic anhydride at 50 8C. Resultant GelMA monomer containing polyethylene glycol (PEG) protected silver nanoparticles were subsequently copolymerized with 2-hydroxypropyl methacrylate (HPMA) at room temperature by redox mechanism. This resulted in a hydrogel copolymer with optimum mechanical stability and moisture retention while inhibiting microbial contamination and FT-IR spectroscopy was used to confirm copolymer formation. Antimicrobial properties of the hydrogel using agar diffusion showed zone of inhibition against Staphylococcus aureus. Surface morphology was observed using scanning electron microscopy (SEM) and elemental analysis was carried out using energydispersive spectroscopy (EDS). Micro-computed tomography (micro-CT) analysis of the hydrogel showed enhancement in the pore size from around 32 m to 48-64 m after incorporation of silver nanoparticles. Degradation of the hydrogel was observed after 48 h when stored in PBS containing collagenase enzyme. In vitro cell culture experiments established absence of cytotoxicity in the hydrogel and nonadherence character to dermal fibroblasts.

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“…As shown in Figure , PEA and PEAB‐1 hydrogels showed highly porous interpenetrating network structures with microscale pores. The three‐dimensional (3D) network with pores can be used as a depot for controlled drug release, cell culture, or micro/nanoparticle delivery . PEAB‐2 and PEAB‐3 aggregates showed fractured microstructures without obvious network morphologies because no hydrogel transition occurred for their solutions.…”

Section: Resultsmentioning

confidence: 99%

Fine tuning the assembly and gel behaviors of PEGylated polypeptide conjugates by the copolymerization of l‐alanine and γ‐benzyl‐l‐glutamate N‐carboxyanhydrides

Song

Zhang

Huang

et al. 2017

J. Polym. Sci. Part A: Polym. Chem.

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Tuning the secondary structure of polypeptide is an effective strategy to modulate the assembly behaviors of polypeptide‐based copolymers. In this study, ring‐opening polymerization of l‐alanine (Ala) and γ‐benzyl‐l‐glutamate (BLG) N‐carboxyanhydrides was adopted using mPEG‐NH2 as the initiator to prepare mPEG‐poly(l‐alanine‐co‐γ‐benzyl‐l‐glutamate) (PEAB) copolymers with various Ala to BLG ratios. 1H NMR spectra and GPC test confirmed their well‐defined chemical structures. FT‐IR spectra indicated that at the powder state, all copolymers adopted both β‐sheet and α‐helical conformations. With the content of PBLG increased, the crystallization temperature and melting points of PEAB copolymers first rose then fell indicated by DSC curves. The self‐assembly of PEAB copolymers in dilute aqueous solution studied by DLS, TEM and circular dichroism spectra showed that PEAB copolymers self‐assembled into nanostructures with diverse morphologies and sizes due to distinct polypeptide conformations. Rheological analysis indicated that the alteration of the polypeptide composition can effectively modulate the modulus of PEAB assemblies in concentrated solutions. In all, copolymerization of two hydrophobic amino acid N‐carboxyanhydrides into the polypeptide block maybe an effective approach for modulating the assembly properties of PEGylated polypeptide. Besides, nanosilver‐encapsulated PEA or PEAB hydrogel showed promising antibacterial effect against Staphylococcus aureus and Bacillus subtillis. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2017, 55, 1512–1523

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Preparation and characterization of silver nanoparticle loaded amorphous hydrogel of carboxymethylcellulose for infected wounds

Cited by 81 publications

References 29 publications

In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels

In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels

Synthesis and characterization of silver nanoparticle incorporated gelatin‐hydroxypropyl methacrylate hydrogels for wound dressing applications

Fine tuning the assembly and gel behaviors of PEGylated polypeptide conjugates by the copolymerization of l‐alanine and γ‐benzyl‐l‐glutamate N‐carboxyanhydrides

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