Preparation and characterization of sericin powder extracted from silk industry wastewater

Wu, Jinhong; Wang, Zhang; Xu, Shengyu

doi:10.1016/j.foodchem.2006.10.042

Cited by 201 publications

(141 citation statements)

References 20 publications

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“…In order to obtain SN, the cocoon shells were cut into small pieces, followed by addition of ultra pure water at a cocoon to water ratio of 1:50 (w/w). Sericin was extracted was dried in a lyophilizator to obtain powder sericin (8,13).…”

Section: Extraction Of Sericin From Native Cocoons Via Hydrothermal Pmentioning

confidence: 99%

“…Ethanol is commonly used to precipitate sericin (6,8,13). However, the volumes that would be required for a real wastewater treatment plant effluent are excessive.…”

Section: Precipitation Of Sericin Recovered From Wastewatermentioning

confidence: 99%

“…The characteristics of sericin obtained directly from the cocoon are well known (5)(6)(7)(8)(9)(10)(11)(12). However, the properties of sericin recovered from textile effluents need to be determined (13)(14)(15). Recovered sericin can be used as a raw material in industries such as biotechnology and textile (16,17).…”

mentioning

confidence: 99%

“…Sericin can be recovered with different techniques such as precipitation in alcohol, enzymatic hydrolysis, freeze-and tray-drying and membrane filtration (2,13,15,18,19 In literature, there seems to exist very few studies on the characterization of sericin recovered from wastewaters. To this end, this study aims to characterize the sericin protein recovered from silk wastewaters generated in cocoon cooking process of textile industry.…”

mentioning

confidence: 99%

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Characterization of sericin protein recovered from silk wastewaters

Çapar¹,

Aygün²

2015

Turk Hij Den Biyol Derg

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Section: Extraction Of Sericin From Native Cocoons Via Hydrothermal Pmentioning

confidence: 99%

“…Ethanol is commonly used to precipitate sericin (6,8,13). However, the volumes that would be required for a real wastewater treatment plant effluent are excessive.…”

Section: Precipitation Of Sericin Recovered From Wastewatermentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Characterization of sericin protein recovered from silk wastewaters

Çapar¹,

Aygün²

2015

Turk Hij Den Biyol Derg

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show abstract

“…In addition, phospholipids and tocopherol existing in the oils may play an important role in protecting the lipids, especially linoleic acid, against oxidation. Wu et al (2007) reported that the 50% scavenging activity by DPPH assay of sericin prepared from silk industry wastewater was 31 mg/mL. Fan et al (2009) have also reported that silk sericin does not show only DPPH radical scavenging activity, but also many antioxidative activities, such as hydroxyl and superoxide radical scavenging, lipid peroxidation of linoleic acid, reducing power, and ferrous ion chelating activity.…”

Section: Free Radical Scavenging Activitymentioning

confidence: 99%

Free radical scavenging and tyrosinase inhibition activity of oils and sericin extracted from Thai native silkworms (Bombyx mori)

Manosroi

Boonpisuttinant

Winitchai

et al. 2010

Pharmaceutical Biology

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Silk Fibroin and Sericin Differentially Potentiate the Paracrine and Regenerative Functions of Stem Cells Through Multiomics Analysis

et al. 2023

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emerging as a potential alternative to these problems. The tissue engineering triad involves the effective integration of cells, scaffolds, and signals for the development of biological substitutes to restore, maintain, and replace injured tissues and organs. [2] The scaffold plays a substantial role in the field of tissue engineering, primarily by providing physical support (structures and substrates) for cells to attach and grow, subsequently resulting in tissue formation. [3] A variety of natural and synthetic biomaterials have been explored to develop tissue-engineered scaffolds. By incorporating exogenous growthstimulating signals, such as growth factors or small molecules, scaffolds can provide specific bioactivities, that is, biochemical signals required for cellular behaviors and tissue regeneration. [4] As biomaterials are essentially comprised of chemical molecules, they are capable of supplying inherent biochemical signals to guide and influence cells. For example, cells residing in tissue-engineered scaffolds can establish cell-biomaterial communication networks partially through biochemical signaling. The degradation products of various biodegradable biomaterials after implantation in vivo also provide specific biochemical signals to the cells in and around the Silk fibroin (SF) and sericin (SS), the two major proteins of silk, are attractive biomaterials with great potential in tissue engineering and regenerative medicine. However, their biochemical interactions with stem cells remain unclear. In this study, multiomics are employed to obtain a global view of the cellular processes and pathways of mesenchymal stem cells (MSCs) triggered by SF and SS to discern cell-biomaterial interactions at an in-depth, high-throughput molecular level. Integrated RNA sequencing and proteomic analysis confirm that SF and SS initiate widespread but distinct cellular responses and potentiate the paracrine functions of MSCs that regulate extracellular matrix deposition, angiogenesis, and immunomodulation through differentially activating the integrin/PI3K/Akt and glycolysis signaling pathways. These paracrine signals of MSCs stimulated by SF and SS effectively improve skin regeneration by regulating the behavior of multiple resident cells (fibroblasts, endothelial cells, and macrophages) in the skin wound microenvironment. Compared to SS, SF exhibits better immunomodulatory effects in vitro and in vivo, indicating its greater potential as a carrier material of MSCs for skin regeneration. This study provides comprehensive and reliable insights into the cellular interactions with SF and SS, enabling the future development of silk-based therapeutics for tissue engineering and stem cell therapy.

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Preparation and characterization of sericin powder extracted from silk industry wastewater

Cited by 201 publications

References 20 publications

Characterization of sericin protein recovered from silk wastewaters

Characterization of sericin protein recovered from silk wastewaters

Free radical scavenging and tyrosinase inhibition activity of oils and sericin extracted from Thai native silkworms (Bombyx mori)

Silk Fibroin and Sericin Differentially Potentiate the Paracrine and Regenerative Functions of Stem Cells Through Multiomics Analysis

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