Characterization of sericin protein recovered from silk wastewaters

Çapar, Gökşen; Aygün, Seylan Saniye

doi:10.5505/turkhijyen.2015.47113

Cited by 14 publications

(8 citation statements)

References 26 publications

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“…The maximal absorption wavelength indicates the absorption peaks of peptides and amino acids in sericin. 19 The amounts of amino acids differed in each type of extracted sericin, but serine was the most abundant in all samples. Serine has a high content of the hydroxyl group, which contributes to the functions of sericin.…”

Section: Discussionmentioning

confidence: 89%

Urea-extracted sericin is potentially better than kojic acid in the inhibition of melanogenesis through increased reactive oxygen species generation

Cherdchom

Sereemaspun

Aramwit

2021

Journal of Traditional and Complementary Medicine

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Background Hyperpigmentation is a skin disorder, which is caused by an excess production of melanin. The reduction in melanin content without causing undesirable effects is required for the treatment of hyperpigmentation. Sericin is increasingly used as a hyperpigmentation treatment because of its antityrosinase activity. However, the various methods of sericin extraction have an effect on the composition and biological properties. The purpose of this study was to investigate the antioxidant and anti-melanogenic properties of sericin using different extraction methods including acid, base, heat, and urea extraction. Methods The chemical properties of extracted sericin were assessed in terms of amino acid components, thermal behavior, and UV–vis absorption. The inhibitory effects of sericin on melanogenesis were explored by determining the melanin content and cellular tyrosinase activity in B16F10 cells. Results Sericin from urea extraction provided different properties when compared with the other extraction methods. Our results indicate that urea-extracted sericin reduced the melanin content and cellular tyrosinase activity more effectively than the other extraction methods. Interestingly, the potential anti-melanogenic activity was more effective than kojic acid, a depigmenting agent used to treat hyperpigmentation. Moreover, treatment of urea-extracted sericin induced reactive oxygen species and subsequently activated antioxidant activity in B16F0 cells. Conclusions Our results present the potential inhibitory effect of urea-extracted sericin on melanogenesis. The therapeutic potential of urea-extracted sericin can be used in the treatment of hyperpigmentation and its complications.

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Section: Discussionmentioning

confidence: 89%

Urea-extracted sericin is potentially better than kojic acid in the inhibition of melanogenesis through increased reactive oxygen species generation

Cherdchom

Sereemaspun

Aramwit

2021

Journal of Traditional and Complementary Medicine

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“…After that, the UV-vis absorption of sericin showed that the maximal absorption wavelength was at around 280 nm. The maximal absorption wavelength indicates the absorption peaks of peptides and amino acids in sericin (18). The amounts of amino acids differed in each type of extracted sericin, but serine was the most abundant in all samples.…”

Section: Discussionmentioning

confidence: 99%

Urea-Extracted Sericin is Potentially Better Than Kojic Acid in the Inhibition of Melanogenesis Through Increased ROS Generation

Cherdchom

Sereemasoun

Aramwit

2020

Preprint

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Background: Hyperpigmentation is a skin disorder, which is caused by an excess production of melanin. The reduction in melanin content without causing undesirable effects is required for the treatment of hyperpigmentation. Sericin is increasingly used as a hyperpigmentation treatment because of its antityrosinase activity. However, the various methods of sericin extraction have an effect on the composition and biological properties. The purpose of this study was to investigate the antioxidant and anti-melanogenic properties of sericin using different extraction methods including acid, base, heat, and urea extraction. Methods: The chemical properties of extracted sericin were assessed in terms of amino acid components, thermal behavior, and UV-vis absorption. The inhibitory effects of sericin on melanogenesis were explored by determining the melanin content and cellular tyrosinase activity in B16F10 cells.Results: Sericin from urea extraction provided different properties when compared with the other extraction methods. Our results indicate that urea-extracted sericin reduced the melanin content and cellular tyrosinase activity more effectively than the other extraction methods. Interestingly, the potential anti-melanogenic activity was more effective than kojic acid, a depigmenting agent used to treat hyperpigmentation.Conclusions: Our results present the potential inhibitory effect of urea-extracted sericin on melanogenesis. Our results support the therapeutic potential of urea-extracted sericin in the treatment of hyperpigmentation and its complications.

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“…All the results indicate that treatment for a period of 120min appears to be sufficient for degumming. Sericin is rich in Serine and the inner layer fibroin has high content of Alanine and glycine [13,25]. The hydrolysate obtained from skein was therefore, subjected to chromatographic run with these amino acid standards.…”

Section: Mcdamsid000585 4(2)2019mentioning

confidence: 99%

“…Molecular weight of the sericin recovered from silk wastewaters ranges from 40-176kDa [13]. Apart from its luster, the fibroin layer is known for its elasticity and tensile strength.…”

Section: Introductionmentioning

confidence: 99%

Pineapple: Potential Source of Proteolytic Enzymes for Degumming of Raw Silk

Shetty¹,

Udupi²,

Madanthyar³

et al. 2019

MCDA

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Pineapple is a well-known reservoir of proteases. Treatment of silk skeins with pineapple juice resulted in degumming of the fibers leading to unmasking of the lustrous inner layer. The juice obtained from raw pineapple was subjected to concentration by saturation with 85% ammonium sulfate. The proteolytic activity of the juice as well as the concentrate was found to effectively degum the skein. The proteases were optimally active at pH 6.6, and 50-55 C. Treatment of the skein with 5U of proteolytic activity at 50 C and pH 6.6 for 120min resulted in weight loss to an extent of 17-18.5%. Release of peptides and amino acids, arginine, histidine and aromatic amino acids was monitored during the process of degumming. The rate of release of peptides and amino acids was relatively higher till 90-120min, reducing thereafter. The enzymatically degummed skeins were found to be more lustrous in comparison to untreated skeins. Conventionally, raw silk skeins are chemically treated to impart luster to the fiber. Enzymatically degummed fibers displayed smoother texture and improved stretch ability in comparison to chemically decoated skeins. Use of pineapple in silk manufacture may boost the agronomy in tropical regions where it is grown extensively.

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Characterization of sericin protein recovered from silk wastewaters

Abstract: Objective: This study aims to determine the characteristics of sericin protein recovered from silk wastewaters. Method

Cited by 14 publications

References 26 publications

Urea-extracted sericin is potentially better than kojic acid in the inhibition of melanogenesis through increased reactive oxygen species generation

Urea-extracted sericin is potentially better than kojic acid in the inhibition of melanogenesis through increased reactive oxygen species generation

Urea-Extracted Sericin is Potentially Better Than Kojic Acid in the Inhibition of Melanogenesis Through Increased ROS Generation

Pineapple: Potential Source of Proteolytic Enzymes for Degumming of Raw Silk

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