Preparation and characterization of new biospecific adsorbents with fatty acids as ligands, usable to retain bovine serum albumin

Adsorptive membranes for bilirubin removal

“…It may also be reasonable to expect that the free bilirubin in bulk solution aggregate onto adsorbed bilirubin [24,[44][45][46]. The use of epoxy-activated gels such as modified cross-linked polybutadiene hydroxyethyl methacrylate for albumin immobilization and their performances as supports in affinity chromatography to retain BR was investigated by Alvarez et al [30] giving maximum bilirubin retentions of 3.1 mg BR/g beads. Sideman et al [13,26] suggested the application of hemoperfusion for the removal of the BR from jaundiced newborn babies by using albumin-deposited macroreticular resins with bilirubin adsorption capacities ranging from 2 to 24 mg BR/g resin.…”

“…The applicability of albumin in BR removal is limited by the large amount of bound BSA required to remove significant amounts of bilirubin. Its high BR specificity and the fact that albumin immobilization onto natural or synthetic polymeric supports improves their biocompatibility [26], however, led to an extensive use of BSA as bioligand in studies of BR removal [27][28][29][30].…”

Functionalised supports with sugar dendritic ligand

“…After concentration, toluene (5 mL) was added and the solution was evaporated in vacuum to remove azeotropically the residual formic acid. Acid-containing dendron (5) was obtained as a viscous oil. Yield was 90% (0.4367 g).…”

“…On the other hand the poly (PB-HEMA) synthetic matrix has been derivatised with different ligands and adapted as supports for affinity chromatography [5,6]. To further exploit the beneficial binding properties of glycodendrimers, we describe herein the synthesis of a dendritic molecule that has organised multivalency.…”

“…Meanwhile, in almost all cases, when specific ligands have been covalently attached on conveniently activated surfaces, proteins can be retained through affinity based interactions [3][4][5][6].…”