Preparation and characterization of N -chitosan as a wound healing accelerator

Tang, Fang‐Fang; Lv, Lingmei; Lu, Fei; Bao, Rong; Li, Zhiquan; Lu, Bitao; Yu, Kun; Liu, Jiawei; Dai, Fangyin; Deng-jun, WU; Lan, Guangqian

doi:10.1016/j.ijbiomac.2016.09.101

Cited by 66 publications

(46 citation statements)

References 59 publications

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“…To overcome these limitations, and improve the final properties of the 3D scaffold, CH nanoforms have been incorporated into CS matrix [24,30]. CH and CS have already been used in preclinical studies for wound healing [31] and bone regeneration [32]. In our previous work, we demonstrated that CH nanoforms, in particular chitin nanocrystals (CHNCs), provide mechanical and topological cues to support the growth of hASCs in CS-based biomaterials for TE [22].…”

Section: Introductionmentioning

confidence: 99%

Adipose-Derived Mesenchymal Stem Cell Chondrospheroids Cultured in Hypoxia and a 3D Porous Chitosan/Chitin Nanocrystal Scaffold as a Platform for Cartilage Tissue Engineering

Zubillaga

Alonso‐Varona

Fernandes

et al. 2020

IJMS

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Articular cartilage degeneration is one of the most common causes of pain and disability in middle-aged and older people. Tissue engineering (TE) has shown great therapeutic promise for this condition. The design of cartilage regeneration constructs must take into account the specific characteristics of the cartilaginous matrix, as well as the avascular nature of cartilage and its cells’ peculiar arrangement in isogenic groups. Keeping these factors in mind, we have designed a 3D porous scaffold based on genipin-crosslinked chitosan/chitin nanocrystals for spheroid chondral differentiation of human adipose tissue-derived mesenchymal stem cells (hASCs) induced in hypoxic conditions. First, we demonstrated that, under low oxygen conditions, the chondrospheroids obtained express cartilage-specific markers including collagen type II (COL2A1) and aggrecan, lacking expression of osteogenic differentiation marker collagen type I (COL1A2). These results were associated with an increased expression of hypoxia-inducible factor 1α, which positively directs COL2A1 and aggrecan expression. Finally, we determined the most suitable chondrogenic differentiation pattern when hASC spheroids were seeded in the 3D porous scaffold under hypoxia and obtained a chondral extracellular matrix with a high sulphated glycosaminoglycan content, which is characteristic of articular cartilage. These findings highlight the potential use of such templates in cartilage tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Adipose-Derived Mesenchymal Stem Cell Chondrospheroids Cultured in Hypoxia and a 3D Porous Chitosan/Chitin Nanocrystal Scaffold as a Platform for Cartilage Tissue Engineering

Zubillaga

Alonso‐Varona

Fernandes

et al. 2020

IJMS

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“…Strategies to address these problems include the modification of CS by combining it with metal (oxide) nanoparticles (such as those of ZnO and Ag, see Table 1). The CS derivatives reported in the literature in the last few years are N-succinyl-chitosan, Oleoyl CS, quaternised CS, O-carboxymethyl CS and N,N,N-trimethyl-chitosan [23–29]. However, impurities such as the myosin residues generated during the extraction of CS can lead to an inflammatory reaction and/or the formation of microabscesses after transplantation [30].…”

Section: Scaffolds From Natural Polymersmentioning

confidence: 99%

Progress in development of bioderived materials for dermal wound healing

Huang

Xie

2017

Regenerative Biomaterials

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Treatment of acute and chronic wounds is one of the primary challenges faced by doctors. Bioderived materials have significant potential clinical value in tissue injury treatment and defect reconstruction. Various strategies, including drug loading, addition of metallic element(s), cross-linking and combining two or more distinct types of materials with complementary features, have been used to synthesize more suitable materials for wound healing. In this review, we describe the recent developments made in the processing of bioderived materials employed for cutaneous wound healing, including newly developed materials such as keratin and soy protein. The focus was on the key properties of the bioderived materials that have shown great promise in improving wound healing, restoration and reconstruction. With their good biocompatibility, nontoxic catabolites, microinflammation characteristics, as well as their ability to induce tissue regeneration and reparation, the bioderived materials have great potential for skin tissue repair.

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“…The decomposition temperature starts at 200°C and the highest decomposition rate is observed at 254°C, and the total weight loss in this stage is 65%. It is quite conspicuous that further modification might lead to lower thermal stability [39,40]. The introduction of functional groups has a significant influence on its thermal stability, and it is due to the changed crystalline structure of starch, especially through the loss of hydrogen bonding between starch chains [18].…”

Section: Tga and Dtg Characterizationmentioning

confidence: 99%