Preparation and characterization of medium-chain fatty acid liposomes by lyophilization

Liu, Chengmei; Yang, Shiyou; Liu, Wei; Wang, Ruilian; Wan, Jie; Li, Weilin

doi:10.3109/08982100903244500

Cited by 8 publications

(3 citation statements)

References 29 publications

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“…The freeze-dried complex nanoliposomes without any cryoprotectant (the control group) exhibited a larger mean particle diameter of (151.4 ± 10.76) nm, and drugs entrapment efficiencies could not be detected because of the damages caused by lyophilization. Similar results were found in our previous report [31], which indicated that the freezing-drying process has a small effect on the liposome size, resulting in a slight increase in average diameter from 250.1 to 263.3 nm. On the other hand, formulations prepared without saccharose as cryoprotectant presented an increase in vesicle size after lyophilization.…”

Section: Resultssupporting

confidence: 92%

Preparation and Characterization of Nanoliposomes Entrapping Medium-Chain Fatty Acids and Vitamin C by Lyophilization

Yang

Liu

et al. 2013

IJMS

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The complex nanoliposomes encapsulating both a hydrophilic drug vitamin C (vit C) and hydrophobic drug medium-chain fatty acids (MCFAs) was prepared by combining double emulsion method with dynamic high pressure microfluidization. The complex nanoliposomes was further freeze-dried under −86 °C for 48 h with sucrose at the sucrose/lipids ratio of 2:1(w/w) in order to enhance its stability. The freeze-dried complex nanoliposomes under the suitable conditions exhibited high entrapment efficiency of MCFAs (44.26 ± 3.34)%, relatively high entrapment efficiency of vit C (62.25 ± 3.43)%, low average size diameter (110.4 ± 7.28) nm and good storage stability at 4 °C for 60 days with slight changes in mean particle diameter and drug entrapment efficiencies. The results of transmission electron microscopy of freeze-dried complex nanoliposomes also showed that the freeze-dried samples with sucrose were stable without great increase in their particle sizes and without destroying their spherical shape. The results indicated that sucrose presented well protection effects in MCFAs-vit C complex nanoliposomes, suggesting the possibility of further usage in commercial liposomes.

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Section: Resultssupporting

confidence: 92%

Preparation and Characterization of Nanoliposomes Entrapping Medium-Chain Fatty Acids and Vitamin C by Lyophilization

Yang

Liu

et al. 2013

IJMS

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“…There have been limited studies on the large-scale preparation of NL from edible lipids by using DHPM from the viewpoint of developing the formulations into an efficient carrier of nutritional materials. In our previous research ( 16 ) , thin-layer dispersion, ethanol injection and reverse-phase evaporation were used to prepare MCFA liposomes, and a new method of thin-layer dispersion combined with DHPM was developed to efficiently prepare MCFA NL with easy-energy-supply property to mice ( 17 ) .…”

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confidence: 99%

Medium-chain fatty acid nanoliposomes suppress body fat accumulation in mice

Liu

Yang

et al. 2011

Br J Nutr

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Medium-chain fatty acids (MCFA) are widely used in diets for patients with obesity. To develop a delivery system for suppressing dietary fat accumulation into adipose tissue, MCFA were encapsulated in nanoliposomes (NL), which can overcome the drawbacks of MCFA and keep their properties unchanged. In the present study, crude liposomes were first produced by the thin-layer dispersion method, and then dynamic high-pressure microfluidisation (DHPM) and DHPM combined with freeze -thawing methods were used to prepare MCFA NL (NL-1 and NL-2, respectively). NL-1 exhibited smaller average size (77·6 (SD 4·3) nm), higher zeta potential (2 40·8 (SD 1·7) mV) and entrapment efficiency (73·3 (SD 16·1) %) and better stability, while NL-2 showed narrower distribution (polydispersion index 0·193 (SD 0·016)). The body fat reduction property of NL-1 and NL-2 were evaluated by short-term (2 weeks) and long-term (6 weeks) experiments of mice. In contrast to the MCFA group, the NL groups had overcome the poor palatability of MCFA because the normal diet of mice was maintained. The body fat and total cholesterol (TCH) of NL-1 (1·54 (SD 0·30) g, P¼0·039 and 2·33 (SD 0·44) mmol/l, P¼0·021, respectively) and NL-2 (1·58 (SD 0·69) g, P¼ 0·041 and 2·29 (SD 0·38) mmol/l, P¼ 0·015, respectively) significantly decreased when compared with the control group (2·11 (SD 0·82) g and 2·99 (SD 0·48) mmol/l, respectively). The TAG concentration of the NL-1 group (0·55 (SD 0·14) mmol/l) was remarkably lower (P¼ 0·045) than the control group (0·94 (SD 0·37) mmol/l). No significant difference in weight and fat gain, TCH and TAG was detected between the MCFA NL and MCFA groups. Therefore, MCFA NL could be potential nutritional candidates for obesity to suppress body fat accumulation.

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“…These properties have been used advantageously in clinics for more than 50 years for treating lipid absorption disorders, undernourished patients, and more recently, subjects with long-chain fatty acid oxidation defects. [15][16][17] For pharmaceutical development and clinical use, several criteria must be fulfilled in terms of size as well as chemical and physical stability. Small-sized liposomes have several advantages as drug delivery systems.…”

Section: Introductionmentioning

confidence: 99%

The comparison of protein-entrapped liposomes and lipoparticles: preparation, characterization, and efficacy of cellular uptake

Chang¹,

Tai²,

Chiang³

et al. 2011

IJN

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Fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA)-loaded polyethylene glycol (PEG)-modified liposomes and lipoparticles with high protein entrapment were developed. The lipid formula of the liposomes contained PEGylated lipids and unsaturated fatty acids for enhancing membrane fluidity and effective delivery into cells. The preparation techniques, lipid content, and PEG-modified lipoparticle ratios were evaluated. The PEG-modified lipoparticles prepared by ethanol injection extrusion (100 nm pore size) achieve a population of blank liposomes with a mean size of 125 ± 2.3 nm and a zeta potential of −12.4 ± 1.5 mV. The average particle size of the PEG-modified lipoparticles was 133.7 ± 8.6 nm with a zeta potential of +13.3 mV. Lipoparticle conformation was determined using transmission electron microscopy and field-emission scanning electron microscopy. The FITC-BSA encapsulation efficiency was dramatically increased from 19.0% for liposomes to 59.7% for lipoparticles. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) results confirmed the preparation process, and an 8-hour leaching test did not harm the protein structure. Once prepared, the physical and chemical stability of the PEG-modified lipoparticle formulations was satisfactory over 90 days. In vitro retention tests indicated that the 50% retention time for the protein-containing lipoparticles was 7.9 hours, substantially longer than the liposomes at 3.3 hours. A Caco-2 cell model was used for evaluating the cytotoxicity and cell uptake efficiency of the PEG-modified lipoparticles. At a lipid content below 0.25 mM, neither the liposomes nor the lipoparticles caused significant cellular cytotoxicity ( P < 0.01) and FITC-BSA was significantly taken up into cells within 60 minutes ( P < 0.01).

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Preparation and characterization of medium-chain fatty acid liposomes by lyophilization

Cited by 8 publications

References 29 publications

Preparation and Characterization of Nanoliposomes Entrapping Medium-Chain Fatty Acids and Vitamin C by Lyophilization

Preparation and Characterization of Nanoliposomes Entrapping Medium-Chain Fatty Acids and Vitamin C by Lyophilization

Medium-chain fatty acid nanoliposomes suppress body fat accumulation in mice

The comparison of protein-entrapped liposomes and lipoparticles: preparation, characterization, and efficacy of cellular uptake

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