Preparation and characterization of magnetic polyporous biochar for cellulase immobilization by physical adsorption

Mo, Haodao; Qiu, Jianhui; Yang, Chao; Zang, Limin; Sakai, Eiichi

doi:10.1007/s10570-020-03125-6

Cited by 34 publications

(16 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most scholars pay more attention to yield data and basic properties than the changes of bio-oil and tail gas during pyrolysis process. The research on the effects of microscopic indexes such as XPS, XRD, and FTIR is also not sufficient and deep enough [22]. In order to better promote the application of biomass pyrolysis technology, it is meaningful to systematically study the impact of pyrolysis process on its properties [23,24].…”

Section: Introductionmentioning

confidence: 99%

The Eco-Friendly Biochar and Valuable Bio-Oil from Caragana korshinskii: Pyrolysis Preparation, Characterization, and Adsorption Applications

et al. 2020

View full text Add to dashboard Cite

Carbonization of biomass can prepare carbon materials with excellent properties. In order to explore the comprehensive utilization and recycling of Caragana korshinskii biomass, 15 kinds of Caragana korshinskii biochar (CB) were prepared by controlling the oxygen-limited pyrolysis process. Moreover, we pay attention to the dynamic changes of microstructure of CB and the by-products. The physicochemical properties of CB were characterized by Scanning Electron Microscope (SEM), BET-specific surface area (BET-SSA), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), and Gas chromatography-mass spectrometry (GC-MS). The optimal preparation technology was evaluated by batch adsorption application experiment of NO3−, and the pyrolysis mechanism was explored. The results showed that the pyrolysis temperature is the most important factor in the properties of CB. With the increase of temperature, the content of C, pH, mesoporous structure, BET-SSA of CB increased, the cation exchange capacity (CEC) decreased and then increased, but the yield and the content of O and N decreased. The CEC, pH, and BET-SSA of CB under each pyrolysis process were 16.64–81.4 cmol·kg−1, 6.65–8.99, and 13.52–133.49 m2·g−1, respectively. CB contains abundant functional groups and mesoporous structure. As the pyrolysis temperature and time increases, the bond valence structure of C 1s, Ca 2p, and O 1s is more stable, and the phase structure of CaCO3 is more obvious, where the aromaticity increases, and the polarity decreases. The CB prepared at 650 °C for 3 h presented the best adsorption performance, and the maximum theoretical adsorption capacity for NO3− reached 120.65 mg·g−1. The Langmuir model and pseudo-second-order model can well describe the isothermal and kinetics adsorption process of NO3−, respectively. Compared with other cellulose and lignin-based biomass materials, CB showed efficient adsorption performance of NO3− without complicated modification condition. The by-products contain bio-soil and tail gas, which are potential source of liquid fuel and chemical raw materials. Especially, the bio-oil of CB contains α-d-glucopyranose, which can be used in medical tests and medicines.

show abstract

Section: Introductionmentioning

confidence: 99%

The Eco-Friendly Biochar and Valuable Bio-Oil from Caragana korshinskii: Pyrolysis Preparation, Characterization, and Adsorption Applications

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Figure 4 shows the N 2 adsorption–desorption isotherm ( Figure 4 a) and the pore size distribution ( Figure 4 b) of the porous biochar, biochar/γ-Fe 2 O 3 , and biochar/γ-Fe 2 O 3 @chitosan using the BJH method. The nitrogen adsorption–desorption isotherms of the samples exhibit a combination of type I and IV shapes according to the IUPAC classification, which explains that the samples contain both micropores and mesopores [ 39 ]. However, the N 2 adsorption capacity of the biochar decreased after modification with γ-Fe 2 O 3 and chitosan.…”

Section: Resultsmentioning

confidence: 99%

“…In order to improve the combination of the porous biochar and the magnetic base, a calcination method was used [ 39 ]. First, 0.1 g of porous biochar, 0.2 mmol of FeCl 3 ·6H 2 O, and 0.1 mmol of FeCl 2 ·4H 2 O were dispersed in 2 mL of an ethanol solution.…”

Section: Methodsmentioning

confidence: 99%

Preparation of Chitosan/Magnetic Porous Biochar as Support for Cellulase Immobilization by Using Glutaraldehyde

Qiu

2020

Polymers

Self Cite

View full text Add to dashboard Cite

In this work, porous biochar was obtained from sugarcane bagasse by alkali activation and pyrolysis and then magnetized with γ-Fe2O3 by calcination. After functionalization with chitosan and activation with glutaraldehyde, the as-prepared chitosan/magnetic porous biochar served as a support to immobilize cellulase by covalent bonds. The immobilization amount of cellulase was 80.5 mg cellulase/g support at pH 5 and 25 °C for 12 h of immobilization. To determine the enzymatic properties, 1% carboxymethyl cellulose sodium (CMC) (dissolved in 0.1 M buffer) was considered as a substrate for hydrolysis at different pH values (3–7) and temperatures (30–70 °C) for 30 min. The results showed that the optimum pH and temperature of the free and immobilized cellulase did not change, which were pH 4 and 60 °C, respectively. The immobilized cellulase had a relatively high activity recovery of 73.0%. However, it also exhibited a higher Michaelis–Menten constant (Km) value and a slower maximum reaction velocity (Vmax) value compared to the free enzyme. In the reusability assay, the immobilized cellulase showed initial glucose productivity of 330.9 mg glucose/g CMC and remained at 86.0% after 10 uses. In conclusion, the chitosan/magnetic porous biochar has great potential applications as a support for enzyme immobilization.

show abstract

“…Besides, it has a moderate reusability, maintaining a 52 % glucose yield after three uses. [65] In another precedent, the use of magnetic γ-Fe 2 O 3 /biochar was employed as porous support (Figure 9) for cellulase by covalent anchoring with the help of chitosan and GA. The porous structure of the biochar was maintained after the growth of iron oxide crystals and chitosan layer and enzyme immobilization, which favored the mass transport of the substrate into the pores.…”

Section: Scheme 3 Hydrolysis Of Insoluble Cellulose With Cellulase Into Soluble Glucosementioning

confidence: 99%

Challenges and Opportunities for the Encapsulation of Enzymes over Porous Solids for Biodiesel Production and Cellulose Valorization into Glucose

Cirujano

Dhakshinamoorthy

2021

ChemCatChem

View full text Add to dashboard Cite

enzymes, via simple adsorption or covalent grafting. Herein, we provide a comprehensive review by comparing the catalytic performance of the enzymes supported over porous solids with respect to the bulk/free enzymes in terms of yields, stability and recycling, highlighting the benefits of the porous support for an optimal biocatalytic performance in the biodiesel production and cellulose valorization into glucose. The final section provides our views for further developments in this field.

show abstract

Preparation and characterization of magnetic polyporous biochar for cellulase immobilization by physical adsorption

Cited by 34 publications

References 34 publications

The Eco-Friendly Biochar and Valuable Bio-Oil from Caragana korshinskii: Pyrolysis Preparation, Characterization, and Adsorption Applications

The Eco-Friendly Biochar and Valuable Bio-Oil from Caragana korshinskii: Pyrolysis Preparation, Characterization, and Adsorption Applications

Preparation of Chitosan/Magnetic Porous Biochar as Support for Cellulase Immobilization by Using Glutaraldehyde

Challenges and Opportunities for the Encapsulation of Enzymes over Porous Solids for Biodiesel Production and Cellulose Valorization into Glucose

Contact Info

Product

Resources

About