Preparation and characterization of macroporous poly(2‐hydroxyethyl methacrylate)‐based biomaterials: Water sorption property and <i>in vitro</i> blood compatibility

Bajpai, A. K.; Kankane, Sanjana

doi:10.1002/app.25580

Cited by 33 publications

(22 citation statements)

References 49 publications

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“…After incubation, the material was fixed in 2.5% gluteraldehyde aqueous solution for 10 min and kept in a 70% ethanol solution in order to avoid dehumidification and microorganism proliferation. The amount and morphology of adhered platelets were analyzed by SEM [21].…”

Section: Platelet Adhesion Testmentioning

confidence: 99%

Designing of hydroxyapatite-gelatin based porous matrix as bone substitute: Correlation with biocompatibility aspects

Bundela¹,

Bajpai²

2008

Express Polym. Lett.

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In the present study polyacrylamide (PAm)-gelatin-hydroxyapatite (HA) composites have been synthesized by suspension polymerization method. The prepared composites were characterized by Fourier transform spectroscopy (FTIR) which revealed the presence of functional groups in the composite. The X-ray diffraction (XRD) studies indicated that HA powder was present in nano size. Thermogravimetric analysis (TGA) revealed that composite is more thermally stable than the polymer matrix alone. The morphology of composite studied by optical microscopy (OPM) and scanning electron microscopy (SEM) suggested that pore size was between 3–20 µm. The composites showed adequately good mechanical properties as evident from the varying compressive strength and modulus in the range 31.57±8.16 MPa and 745±388 MPa, respectively. The water sorption behavior was found to be dependent on the chemical composition of the matrix and the sorption data were used to calculate network parameters. The porosity of composite varied between 4 to 30.66%.The in vitro blood compatibility indicated that the adsorption of bovine serum albumin (BSA) varied from 0.11 to 0.24 mg•g–1, the percentage haemolysis was between 2.4 to 6.9% and the weight of blood clot formed on the composite surfaces were found in the range 11 to 52 mg

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Section: Platelet Adhesion Testmentioning

confidence: 99%

Designing of hydroxyapatite-gelatin based porous matrix as bone substitute: Correlation with biocompatibility aspects

Bundela¹,

Bajpai²

2008

Express Polym. Lett.

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“…Scanning electron microscopy ℃ (SEM, JSM-6320F, Japan) and transmission electron microscopy (TEM, Hitachi H-7010A, Japan) were applied to observe the size and morphology of microspheres. The hydrodynamic radius (R h ) and size distribution of microspheres determined by laser light scattering (LLS, ALV-5000, Germany) at 25 with laser wavelength of 632. ℃ 8 nm, scattering angle 90° were simulated by the non-linear simulation CONTIN.…”

Section: Characterization Methodsmentioning

confidence: 99%

“…Graft copolymer microspheres are widely used in many fields such as the catalyst carrier, 23 drug delivery system 24 and biomedicine 25 due to their well-modified surface structure and good dispersion stability. The microspheres can be prepared through soap-free emulsion copolymerization, 26 dispersion copolymerization 27 and so on.…”

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confidence: 99%

Preparation of P4VP‐g‐PSt Copolymer Microspheres with Controllable Size by Atom Transfer Radical Polymerization Synthesized Poly(4‐vinylpyridine) Macromonomer

Chu

et al. 2009

Chin. J. Chem.

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Poly(4-vinylpyridine) macromonomer (St-P4VP) with a styryl end group was synthesized by atom transfer radical polymerization (ATRP) of 4-vinylpyridine using p-(chloromethyl)styrene (CMSt) as functional initiator, CuCl as catalyst and tris[2-(dimethylamino)ethyl]amine (Me 6 TREN) as ligand in 2-propanol. The structure of St-P4VP macromonomer was identified by proton nuclear magnetic resonance ( 1 H NMR). The result of gel permeation chromatography (GPC) illustrated that the number-average molecular weight of St-P4VP could be controlled by adjusting polymerization conditions. Poly(4-vinylpyridine) grafted polystyrene microspheres (P4VP-g-PSt) were then prepared by dispersion copolymerization of styrene with St-P4VP macromonomers. The effects of polymerization reaction parameters such as medium polarity, concentration of St-P4VP macromonomer and polymerization temperature on the sizes and size distribution of P4VP-g-PSt microspheres were investigated. The results of transmission electron microscopy (TEM), scanning electron microscopy (SEM) and laser light scattering (LLS) indicated that mono-dispersed P4VP-g-PSt microspheres with average diameters of 100-200 nm could be obtained when the molar ratio of St to St-P4VP was 0.25∶100 in ethanol/water mixed solvents (V/V＝80∶20) at 60 ℃. Such kind of graft copolymer microspheres was expected to be applied to many fields such as drug delivery system and protein adsorption/separation system due to their particular structure.

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“…Therefore, higher absorbance values indicate improved thromboresistance of material. In the present thrombogenicity assay, the blood solutions obtained after 10,20,30,40, and 50 min were diluted to half of its concentration and the corresponding absorbance values are shown in Figure 6.…”

Section: Thrombogenicity Assaymentioning

confidence: 99%

“…Thrombus formation and rupture of red blood cell (RBC) are two major concerns in the blood compatibility of a material. 30 Thrombus formation and RBC rupture due to PADMA membranes were evaluated by thrombogenicity assay and hemolysis assay, respectively. Diffusion experiments were carried out with PADMA membranes, taking salicylic acid (SA) as a model drug.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of poly(acrylic acid‐co‐N‐[3‐(dimethylamino)propyl]‐methacrylamide) hydrogel membranes for biomedical applications

Das¹,

Ray²

2008

J of Applied Polymer Sci

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A series of new hydrogel membranes with different compositions of acrylic acid (AAc) and N‐[3‐(dimethylamino)propyl]‐methacrylamide (DMAPMA) were prepared by aqueous copolymerization, without using chemical crosslinker or radiation. Chemical structure of the membranes (PADMAs) was characterized by Fourier transform infrared spectroscopy (FTIR). Swelling experiments were carried out in simulated body fluid (SBF) at 37 ± 1°C to investigate degree of swelling, dimensional stability, and pore size of the PADMA membranes. In SBF, the variation of pore size with membrane composition was monitored by optical microscopic technique. Morphology of the membranes was characterized, before and after exposure to SBF, by scanning electron microscopy (SEM). It was observed that the membranes are composed of closely packed nanogels of ∼200 nm. Macroporous network structure of the SBF‐swollen PADMA was also observed to be composed of interconnected nanogels. Blood compatibility of the PADMA membranes was evaluated in vitro, by performing hemolysis assay and thrombogenicity assay. The extent of hemolysis due to PADMA membranes was found to be <2%, which ensured that all of the membranes were highly hemocompatible. Salicylic acid (SA) was chosen as a model drug. Diffusion coefficient of SA through PADMA membranes was investigated. It was observed that membrane composition regulates both pore size and drug diffusion. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008

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Preparation and characterization of macroporous poly(2‐hydroxyethyl methacrylate)‐based biomaterials: Water sorption property and in vitro blood compatibility

Cited by 33 publications

References 49 publications

Designing of hydroxyapatite-gelatin based porous matrix as bone substitute: Correlation with biocompatibility aspects

Designing of hydroxyapatite-gelatin based porous matrix as bone substitute: Correlation with biocompatibility aspects

Preparation of P4VP‐g‐PSt Copolymer Microspheres with Controllable Size by Atom Transfer Radical Polymerization Synthesized Poly(4‐vinylpyridine) Macromonomer

Synthesis and characterization of poly(acrylic acid‐co‐N‐[3‐(dimethylamino)propyl]‐methacrylamide) hydrogel membranes for biomedical applications

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