Preparation and characterization of Keratin-PEG conjugate-based micelles as a tumor microenvironment-responsive drug delivery system

Du, Jinsong; Wu, Qiong; Li, Yanmei; Liu, Pengcheng; Han, Xiao; Wang, Lijuan; Yuan, Jiang; Meng, Xianwei; Xiao, Yinghong

doi:10.1080/09205063.2020.1747044

Cited by 14 publications

(9 citation statements)

References 47 publications

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“…PEG-block copolymers normally exhibit negligible cytotoxicity at relatively low concentrations. However, at high concentrations (especially up to 1 mg/mL), decreased cell viability has been observed in cells treated with various types of PEG-block copolymers. − A recent study reported that an amphiphilic polymer of methoxy-PEG-block-triacontanol showed significant cytotoxicity on both MCF-7 and A549 cancer cell lines when concentrations reached 0.25 mg/mL . The Pal-pMPC polymer may have excellent cytotoxicity because it mimics the phospholipids in the biological membranes.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Characterization of Palmitoyl-block-poly(methacryloyloxyethyl phosphorylcholine) Polymer Micelles for Anticancer Drug Delivery

Zhang

Song

et al. 2022

Biomacromolecules

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We report the synthesis and characterization of an amphiphilic polymer comprising a hydrophobic palmitoyl (Pal) group and a zwitterionic poly(2-methacryloyloxyethyl phosphorylcholine) (pMPC) block, which is capable of forming micelles as a drug carrier system for delivering hydrophobic anticancer drugs such as doxorubicin (DOX). We hypothesize that the sharp polarity contrast between the Pal domain and the pMPC block would strengthen the micelles and improve the drug loading capacity, while the pMPC shells improve the micelle stability and cellular uptake efficiency. In this study, the Pal-pMPC polymer was characterized and compared with a Pal-poly(ethylene glycol) (Pal-PEG) polymer in terms of their micelle formation, cytotoxicity, and drug loading of DOX. The DOX-loaded Pal-pMPC micelles were further evaluated for the cellular uptake and anticancer activities in cell culture systems including the non-multidrug-resistance HeLa cell line and the multidrug-resistance AT3B-1 cell line. The results showed that the Pal-pMPC polymer had a minimal toxicity. The Pal-pMPC micelles exhibited higher drug loading capacity and enhanced cellular internalization efficiency compared to micelles formed by the Pal-PEG polymer. It was also found that DOX-loaded Pal-pMPC micelles exhibited a more efficient anticancer effect than Pal-PEG micelles in multidrug-resistance cancer cells in an environment with fetal bovine serum.

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Section: Resultsmentioning

confidence: 99%

Synthesis and Characterization of Palmitoyl-block-poly(methacryloyloxyethyl phosphorylcholine) Polymer Micelles for Anticancer Drug Delivery

Zhang

Song

et al. 2022

Biomacromolecules

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“…Both solutions were mixed and transferred into a high-pressure reactor, and heated at 130 °C for 6 h. The product was collected by centrifugation, and washed with ethanol and deionized water (DI). The keratin containing of numerous carboxyl groups was prepared according to the method previously reported by our group (Du et al, 2020). Then, 40 mg Cu-MOFs containing amino groups, 40 mg keratin containing carboxyl groups, 10 mg EDCHCl and 6 mg NHS were mixed in 16 mL DI and stirred at constant speed for 4 h. The reaction was then terminated and the solution was centrifuged at 10,000 rpm for 10 min, and the pellet was freeze-dried.…”

Section: Preparation Of Cu-mofs@keratinmentioning

confidence: 99%

“…Compared with PEG and zwitterionic polymer, human hair keratin can effectively avoid the immune response and allergic reactions (Du et al, 2021a). On the other hand, it can easily disintegrate in the tumor microenvironment (TME) in response to low pH, and high levels of glutathione (GSH) and trypsin, which is conducive to release of the drug cargo in the tumor tissues (Du et al, 2020). Wang et al fabricated a DDS consisting of sulfhydryl-linked keratin, which was highly stable in a simulated blood environment .…”

Section: Introductionmentioning

confidence: 99%

Multi-stimuli responsive Cu-MOFs@Keratin drug delivery system for chemodynamic therapy

Chen

Yuan

et al. 2023

Front. Bioeng. Biotechnol.

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Although the potential of metal-organic framework (MOF) nanoparticles as drug delivery systems (DDS) for cancer treatment has been established by numerous studies, their clinical applications are still limited due to relatively poor biocompatibility. We fabricated a multifunctional Cu-MOFs@Keratin DDS for loaded drug and chemodynamic therapy (CDT) against tumor cells. The Cu-MOFs core was prepared using a hydrothermal method, and then loaded with the anticancer drug DOX and wrapped in human hair keratin. The Cu-MOFs@Keratin was well characterized by transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and X-ray photoelectron spectroscopy (XPS). Characterization and pharmacokinetic studies of Cu-MOFs@Keratin were performed in vitro and in vivo. The keratin shell reduced the cytotoxicity and potential leakage of Cu-MOFs to normal cells, and allowed the drug-loaded nanoparticles to accumulate in the tumor tissues through enhanced permeability and retention effect (EPR). The particles entered the tumor cells via endocytosis and disintegrated under the stimulation of intracellular environment, thereby releasing DOX in a controlled manner. In addition, the Cu-MOFs produced hydroxyl radicals (·OH) by consuming presence of high intracellular levels of glutathione (GSH) and H2O2, which decreased the viability of the tumor cells.

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“…Hair is mainly composed of keratin and melanin. Keratin is fundamental for some natural biomimetic materials, for it has wide applications in bone regeneration and hemostasis [ 14 , 15 ]. Melanin also has good photothermal effects and has been reported to treat tumors and infections with considerable curative effect [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Cell membrane-coated human hair nanoparticles for precise disease therapies

Zhang

Xia

et al. 2022

J Nanobiotechnol

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Precision medicine is the ultimate goal for current disease therapies, including tumor and infection. The lack of specific targeted drugs for liver cancer and the lack of specific anti-infective drugs in the treatment of diabetic foot ulcer with infection (DFI) are the representative obstacles in those 2 major diseases currently plaguing human beings. Inventing natural biocompatible polymers derived from natural materials is one of the main development directions of current bio-medical materials. Though previous studies have demonstrated the potential application values of human black hair-derived nanoparticles (HNP) in cancer, methicillin-resistant Staphylococcus aureus (MRSA) infection, and thrombosis scenarios treatments, it still has not solved the problem of low local therapeutic concentration and general targeting ability. Here, we firstly modified the HNP with membrane encapsulations, which endowed these dual-pure natural bio-fabricated materials with better targeting ability at the disease sites with no reduction in photothermal therapy (PTT) effect. HNP coated by red blood cell membrane loaded with DSPE-PEG-cRGD peptide for the therapeutic application of liver cancer greatly prolonged in vivo circulation time and enhanced local targeting efficacy as well as low toxicity; HNP coated by the murine macrophage cell membrane (RAWM) for the DFIs treatment greatly promoted the adhesive ability of HNP on the bacteria and thereby improved the killing effect. Briefly, the appropriate cell membranes camouflaged HNP nanomedicine has the characteristics of excellent photothermal effect, an all-natural source with excellent biocompatibility and easy access, which is expected to have huge potential in both benign and malignant diseases.

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Preparation and characterization of Keratin-PEG conjugate-based micelles as a tumor microenvironment-responsive drug delivery system

Cited by 14 publications

References 47 publications

Synthesis and Characterization of Palmitoyl-block-poly(methacryloyloxyethyl phosphorylcholine) Polymer Micelles for Anticancer Drug Delivery

Synthesis and Characterization of Palmitoyl-block-poly(methacryloyloxyethyl phosphorylcholine) Polymer Micelles for Anticancer Drug Delivery

Multi-stimuli responsive Cu-MOFs@Keratin drug delivery system for chemodynamic therapy

Cell membrane-coated human hair nanoparticles for precise disease therapies

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