Preparation and Characterization of Hydrophilic PLGA/Tween 80 Films and Porous Scaffolds

Oh, Se Heang; Cho, Sang; Liu, Jin

doi:10.1080/15421400490479352

Cited by 9 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taken together, the weight loss of poly(LLA‐ co ‐CL)/Tween 80 scaffolds, in combination with dynamic cell culture, implies a release of Tween 80 from the scaffolds. These results are in accordance with previous reports indicating that release of Tween 80 from copolymer films and loss of Tween 80 are most likely attributable to rapid degradation of polymer material from the surface, leading to further exposure and release of Tween 80.…”

Section: Discussionsupporting

confidence: 93%

“…Tween 80 was selected as the hydrophilic additive because it does not readily leach out into water or cell culture medium. In agreement with previous findings it was found that Tween 80 blends readily in to polymer: hydrophobic interaction between the hydrophobic tail of Tween 80 and the polymer main chain provides homogenous distribution and stability of Tween 80 within the copolymer . Moreover, a low concentration of Tween 80 increases the hydrophilicity of poly(LLA‐ co ‐CL) scaffolds without any changes in surface topography and thermal properties .…”

Section: Discussionsupporting

confidence: 91%

“…Fractal dimension expresses the roughness or complexity of the microstructure, which is the repetitive pattern of gray scale configurations, as previously described and is related to the microstructure properties of polymer scaffolds or trabecular bone. Tween 80 concentrations might potentially affect the mechanical properties of the copolymers . The plasticizing effect of Tween 80 probably depends on the concentration in the polymer matrix; the higher the Tween 80 concentration, the softer the polymer.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Surfactant tuning of hydrophilicity of porous degradable copolymer scaffolds promotes cellular proliferation and enhances bone formation

Yassin

Leknes

Sun

et al. 2016

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Poly(l-lactide-co-ɛ-caprolactone) (poly(LLA-co-CL)) has been blended with Tween 80 to tune the material properties and optimize cell-material interactions. Accordingly, the aims of this study were fourfold: to evaluate the effect of low concentrations of Tween 80 on the surface microstructure of 3D poly(LLA-co-CL) porous scaffolds: to determine the effect of different concentrations of Tween 80 on proliferation of bone marrow stromal cells (BMSCs) in vitro under dynamic cell culture at 7 and 21 days; to assess the influence of Tween 80 on the degradation rate of poly(LLA-co-CL) at 7 and 21 days; and in a subcutaneous rat model, to evaluate the effect on bone formation of porous scaffolds modified with 3% Tween 80 at 2 and 8 weeks. Blending 3% (w/w) Tween 80 with poly(LLA-co-CL) improves the surface wettability (p < 0.001). Poly(LLA-co-CL)/3% Tween 80 shows significantly increased cellular proliferation at days 7 and 21 (p < 0.001). Moreover, the presence of Tween 80 facilitates the degradation of poly(LLA-co-CL). Two weeks post-implantation, the poly(LLA-co-CL)/3% Tween 80 scaffolds exhibit significant mRNA expression of Runx2 (p = 0.004). After 8 weeks, poly(LLA-co-CL)/3% Tween 80 scaffolds show significantly increased de novo bone formation, demonstrated by μ-CT (p = 0.0133) and confirmed histologically. It can be concluded that blending 3% (w/w) Tween 80 with poly (LLA-co-CL) improves the hydrophilicity and osteogenic potential of the scaffolds. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2049-2059, 2016.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Surfactant tuning of hydrophilicity of porous degradable copolymer scaffolds promotes cellular proliferation and enhances bone formation

Yassin

Leknes

Sun

et al. 2016

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

show abstract

“…31 The use of Tween 80 as an additive to increase polymer hydrophilicity has previously been reported with poly(DL-lactide-co-glycolic acid) (PLGA). 32 The PLGA/Tween 80 film was prepared by solvent casting using 10% Tween 80, which resulted in a rapid decrease of hydrophobicity and increased human chondrocyte proliferation in cultures up to 3 weeks.…”

Section: ■ Introductionmentioning

confidence: 99%

Surfactant as a Critical Factor When Tuning the Hydrophilicity in Three-Dimensional Polyester-Based Scaffolds: Impact of Hydrophilicity on Their Mechanical Properties and the Cellular Response of Human Osteoblast-Like Cells

et al. 2014

View full text Add to dashboard Cite

In tissue engineering, the hydrophilicity of porous scaffolds is essential and influences protein and cell adhesion as well as nutrient diffusion into the scaffold. The relative low hydrophilicity of degradable polyesters, which limits diffusion of nutrients, is a major drawback in large porous scaffolds of these materials when used for bone tissue engineering and repair of critical size defects. Designing porous biodegradable polymer scaffolds with improved hydrophilicity, while maintaining their mechanical, thermal, and degradation properties is therefore of clinical interest. Here, surfactants were used to tune the hydrophilicity and material properties. A total of 3-20% (w/w) of surfactant, polysorbate 80 (Tween 80), was used as an additive in poly(l-lactide-co-1,5-dioxepan-2-one) [poly(LLA-co-DXO)] and poly(l-lactide)-co-(ε-caprolactone) [poly(LLA-co-CL)] scaffolds. A significantly decreased water contact angle was recorded for all the blends and the crystallinity, glass transition temperature and crystallization temperature were reduced with increased amounts of surfactant. Copolymers with the addition of 3% Tween 80 had comparable mechanical properties as the pristine copolymers. However, the E-modulus and tensile stress of copolymers decreased significantly with the addition of 10 and 20% Tween 80. Initial cell response of the material was evaluated by seeding human osteoblast-like cells (HOB) on the scaffolds. The addition of 3% Tween 80 did not significantly influence cell attachment or proliferation, while 20% Tween 80 significantly inhibited osteoblast proliferation. RT-PCR results showed that 3% Tween 80 stimulated mRNA expression of alkaline phosphatase (ALP), osteoprotegerin (OPG), and bone morphogenetic protein-2 (BMP-2).

show abstract

“…PCL and PCL/Pluronic F127 scaffolds were fabricated using a modified melt-molding particulate-leaching method, as described elsewhere. 34 In brief, 100% PCL or PCL/Pluronic F127 mixture (95/5, w/w) was dissolved in methylene chloride (MC; 10% wt/wt; Junsei, Japan) at room temperature. In this case, Pluronic F127 was used as a hydrophilic additive.…”

Section: Fabrication Of Scaffoldsmentioning

confidence: 99%

Chondrogenesis using mesenchymal stem cells and PCL scaffolds

Kim

Lee

2009

J Biomedical Materials Res

View full text Add to dashboard Cite

We tested the in vitro feasibility of porous PCL (poly(epsilon-caprolactone)) as a scaffold for cartilage tissue engineering from mesenchymal stem cells (MSCs) and determined the effects of various surface treatments. Three porous PCL scaffold modifications were examined: (1) PCL/Pluronic F127, (2) PCL/collagen, and (3) PCL/Pluronic F127/collagen, in addition to (4) PCL-only. MSCs (5 x 10(5)) were seeded in PCL scaffolds of pore size 100-150 microm, and after 3 weeks of in vitro culture, MSC-scaffolds were investigated for gross appearance, DNA amount, glycosaminoglycan (GAG) content, chondrogenic gene expression, and histology. Grossly, the cell-scaffold complexes became harder, and were more easily manipulated with a forceps after 3 weeks of culture. The three surface-treated scaffolds had higher DNA contents than did the PCL-only scaffold, and the GAG contents in PCL/collagen and PCL/F127/collagen scaffolds were higher than those seen in the PCL-only scaffold. Real-time PCR showed that Sox-9 and COL2A1 mRNA levels were remarkably elevated in PCL/collagen and PCL/F127/collagen scaffolds versus the PCL-only scaffold. On the other hand, Col1A1 and Col10A1 mRNA levels were lower in the three modified PCL scaffolds than in the PCL-only scaffold. Histological findings generally concurred with GAG and RT-PCR findings, and demonstrated the affinity of PCL-based scaffolds for MSCs and the potentials of these scaffold to induce chondrogenic differentiation. Cells showed more differentiated appearance and more abundant extracellular matrix formation in PCL/collagen and PCL/collagen/F127 scaffolds. Our findings suggest that PCL-based porous scaffolds may be useful carriers for MSC transplantation in the cartilage tissue engineering field, and that collagen-based surface modifications further enhance the chondrogenic differentiation of MSCs.

show abstract

Preparation and Characterization of Hydrophilic PLGA/Tween 80 Films and Porous Scaffolds

Cited by 9 publications

References 19 publications

Surfactant tuning of hydrophilicity of porous degradable copolymer scaffolds promotes cellular proliferation and enhances bone formation

Surfactant tuning of hydrophilicity of porous degradable copolymer scaffolds promotes cellular proliferation and enhances bone formation

Surfactant as a Critical Factor When Tuning the Hydrophilicity in Three-Dimensional Polyester-Based Scaffolds: Impact of Hydrophilicity on Their Mechanical Properties and the Cellular Response of Human Osteoblast-Like Cells

Chondrogenesis using mesenchymal stem cells and PCL scaffolds

Contact Info

Product

Resources

About