Preparation and characterization of high purity β-chitin from squid pens ( Loligo chenisis )

Cuong, Hoang Ngoc; Nguyen, Van Hoa; Trung, Trang Sĩ

doi:10.1016/j.ijbiomac.2016.08.085

Cited by 85 publications

(49 citation statements)

References 23 publications

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“…Because of the low ash content, demineralization of squid pens was omitted (Cortizo, Berghoff, & Alessandrini, ; Cuong, Minh, Van Hoa, & Trung, ). Deproteinization was carried out using 1.0 M NaOH with the aid of ultrasonic treatment designed with the help of central composite design (CCD) of response surface methodology (RSM; Table ).…”

Section: Methodsmentioning

confidence: 99%

Ultrasound‐Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity

Singh

Benjakul

Prodpran

2019

Journal of Food Science

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Chitosan from squid (Loligo formosana) pens were prepared and characterized. First, ultrasonication condition was optimized for deproteinization of squid pens using central composite design (CCD) of response surface methodology (RSM). Squid pens were ultrasonicated at amplitude 69% for 41.46 min at the solid/solvent ratio of 1:18 yielded 34% (w/w) chitin with the lowest remaining protein. Therefore, ultrasonication effectively reduced the extraction time for chitin production from squid pens as compared to traditional method (5 hr). When the resultant chitin was subjected to deacetylation at different temperatures and times, yield and degree of deacetylation (DDA) of chitosan were in the range of 50% to 65% (w/w) and 78% to 90%. Intrinsic viscosity and molecular weight (MW) of chitosan were in the range of 3.2 to 6.52 dL/g and 1.2 × 10 5 to 3.2 × 10 5 Da, respectively. All the chitosans with different DDA were able to bind oil droplets under the mimicked pH condition of gastrointestinal tract. Chitosan produced by deacetylation at 130°C for 2 hr (CH130-2) showed the optimum yield (54%) and had medium MW (1.5 × 10 5 Da). DDA of CH130-2 determined using 1 H-NMR was 89%, which was similar to that (87%) obtained from FTIR. XRD results showed destruction of chitin structure and decreased crystallinity index from 55% to 27% after deacetylation. CH130-2 stabilized the emulsion under the simulated gastrointestinal conditions. Therefore, it could be used as dietary fiber to control the adsorption of fat/oil in the human digestive tract.Practical Application: Chitin and chitosan are marketable products manufactured from crustacean shells. However, extraction of chitin is time consuming. Ultrasonication has been used for extraction of various biomolecules from different sources. It effectively lowers the processing time and enhances extraction yield. Therefore, application of ultrasonication with optimized condition using RSM could reduce extraction time and enhance yield of chitin from squid pen. Chitin from squid pen could be further converted to chitosan with high DDA. Chitosan was able to act as a dietary fiber and reduce fat absorption in gastrointestinal tract. Thus, this information is of benefit for squid processing industry to exploit squid pen, a processing byproduct.

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Section: Methodsmentioning

confidence: 99%

Ultrasound‐Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity

Singh

Benjakul

Prodpran

2019

Journal of Food Science

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“…compared to total source mass, chitin contents, and other major organic and inorganic constituents listed. Data from [14,[18][19][20][21][22][23][24][25][26][27][28][29][30]. Both crustacean and fungal chitin have a similar molecular structure to cellulose, which is the structural component of the primary cell wall of all green plants, algae, and oomycetes.…”

Section: Introductionmentioning

confidence: 99%

Crab vs. Mushroom: A Review of Crustacean and Fungal Chitin in Wound Treatment

et al. 2020

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Chitin and its derivative chitosan are popular constituents in wound-treatment technologies due to their nanoscale fibrous morphology and attractive biomedical properties that accelerate healing and reduce scarring. These abundant natural polymers found in arthropod exoskeletons and fungal cell walls affect almost every phase of the healing process, acting as hemostatic and antibacterial agents that also support cell proliferation and attachment. However, key differences exist in the structure, properties, processing, and associated polymers of fungal and arthropod chitin, affecting their respective application to wound treatment. High purity crustacean-derived chitin and chitosan have been widely investigated for wound-treatment applications, with research incorporating chemically modified chitosan derivatives and advanced nanocomposite dressings utilizing biocompatible additives, such as natural polysaccharides, mineral clays, and metal nanoparticles used to achieve excellent mechanical and biomedical properties. Conversely, fungi-derived chitin is covalently decorated with -glucan and has received less research interest despite its mass production potential, simple extraction process, variations in chitin and associated polymer content, and the established healing properties of fungal exopolysaccharides. This review investigates the proven biomedical properties of both fungal- and crustacean-derived chitin and chitosan, their healing mechanisms, and their potential to advance modern wound-treatment methods through further research and practical application.

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“…Structural aminopolysaccharide chitin is recognized to occur as the basic component in both non-mineralized and mineralized skeletal formations of the cell walls of diverse fungi [ 1 – 3 ], diatoms [ 4 ], sponges [ 5 – 9 ], corals [ 10 ], annelids [ 11 ], molluscs [ 12 , 13 ], and arthropods (see for review [ 14 ]). This ancient biopolymer is typically cross-linked due to the complex linkage with pigments, lipids, other polysaccharides, peptides and proteins.…”

Section: Introductionmentioning

confidence: 99%

Discovery of chitin in skeletons of non-verongiid Red Sea demosponges

et al. 2018

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Marine demosponges (Porifera: Demospongiae) are recognized as first metazoans which have developed over millions of years of evolution effective survival strategies based on unique metabolic pathways to produce both biologically active secondary metabolites and biopolymer-based stiff skeletons with 3D architecture. Up to date, among marine demosponges, only representatives of the Verongiida order have been known to synthetize biologically active substances as well as skeletons made of structural polysaccharide chitin. This work, to our knowledge, demonstrates for the first time that chitin is an important structural component within skeletons of non-verongiid demosponges Acarnus wolffgangi and Echinoclathria gibbosa collected in the Red Sea. Calcofluor white staining, FTIR and Raman analysis, ESI-MS, SEM, and fluorescence microscopy as well as a chitinase digestion assay were applied in order to confirm, with strong evidence, the finding of α-chitin in the skeleton of both species. We suggest that, the finding of chitin within these representatives of Poecilosclerida order is a promising step in the evaluation of these sponges as novel renewable sources for both biologically active metabolites and chitin, which are of prospective application for pharmacology and biomedicine.

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Preparation and characterization of high purity β-chitin from squid pens ( Loligo chenisis )

Cited by 85 publications

References 23 publications

Ultrasound‐Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity

Ultrasound‐Assisted Extraction of Chitosan from Squid Pen: Molecular Characterization and Fat Binding Capacity

Crab vs. Mushroom: A Review of Crustacean and Fungal Chitin in Wound Treatment

Discovery of chitin in skeletons of non-verongiid Red Sea demosponges

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