Preparation and characterization of DNA hydrogel bead as selective adsorbent of dioxins

Liu, Xiangdong; Murayama, Yoko; Matsunaga, Masaji; Nomizu, Motoyoshi; Nishi, Norio

doi:10.1016/j.ijbiomac.2005.01.008

Cited by 32 publications

(14 citation statements)

References 18 publications

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“…Hydrogel microparticles can be prepared by using either emulsion templated polymerization or lithographic techniques. 28,[31][32][33] We chose the former method to achieve a high yield. In a typical synthesis, Acrydite-modified Hg 2+ -binding DNA, acrylamide, and bisacrylamide were mixed.…”

Section: Resultsmentioning

confidence: 99%

Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine

Helwa

Dave

Froidevaux

et al. 2012

ACS Appl. Mater. Interfaces

120

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These authors contributed equally to the work. AbstractWith a low optical background, high loading capacity, and good biocompatibility, hydrogels are ideal materials for immobilization of biopolymers to develop optical biosensors. We recently immobilized mercury and lead binding DNAs within a monolithic gel and demonstrated ultra-sensitive visual detection of these heavy metals. The high sensitivity was attributed to the enrichment of the analytes into the gels.The signaling kinetics was slow, however, taking about one hour to obtain a stable optical signal due to a long diffusion distance. In this work we aim to understand the analyte enrichment process and improve the signaling kinetics by preparing hydrogel microparticles. DNA-functionalized gel beads were synthesized using an emulsion polymerization technique and most of the beads were between 10 and 50 μm. Acrydite-modified DNA was incorporated by co-polymerization. Visual detection of 10 nM Hg 2+ was still achieved and a stable signal was obtained in just two minutes. The gel beads could be spotted to form a microarray and dried for storage. A new visual sensor for adenosine was designed and immobilized within the gel beads. The adenosine aptamer binds its target about 1000-fold less tightly compared to the mercury binding DNA, allowing a comparison to be made on analyte enrichment by aptamer-functionalized hydrogels.

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Section: Resultsmentioning

confidence: 99%

Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine

Helwa

Dave

Froidevaux

et al. 2012

ACS Appl. Mater. Interfaces

120

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show abstract

“…Polyacrylamide hydrogel microparticles are prepared using the inverse emulsion polymerization method [84][85][86]. First, a gel stock is prepared by dissolving acrylamide (9 g) and N,N'-methylenebisacrylamide (1 g) in a total volume of 50 mL.…”

Section: Hydrogel Microparticlesmentioning

confidence: 99%

Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

Khimji

Kelly

Helwa

et al. 2013

Methods

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“…For example, glass-bead-immobilized DNA has been reported to be effective for the removal of dioxin and polychlorinated biphenyl derivatives [4][5][6][7]. Other studies have demonstrated the DNAassisted removal of heavy metal ions by precipitation of DNA complexes [8,9], removal of toxic aromatic organic compounds from wastewater through ultra-filtration and adsorption [10,11] as well as from contaminated soil through washing with DNA solutions [12].…”

Section: Introductionmentioning

confidence: 99%

Uptake of aromatic compounds by DNA: Toward the environmental application of DNA for cleaning water

Fernández-Solis

Kuroda

Zinchenko

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Preparation and characterization of DNA hydrogel bead as selective adsorbent of dioxins

Cited by 32 publications

References 18 publications

Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine

Aptamer-Functionalized Hydrogel Microparticles for Fast Visual Detection of Mercury(II) and Adenosine

Visual optical biosensors based on DNA-functionalized polyacrylamide hydrogels

Uptake of aromatic compounds by DNA: Toward the environmental application of DNA for cleaning water

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