Preparation and characterization of cross-linked enzyme aggregates (CLEAs) of Brassica rapa peroxidase

Tandjaoui, Nassima; Tassist, Amina; Abouseoud, Mahmoud; Couvert, Annabelle; Amrane, Abdeltif

doi:10.1016/j.bcab.2014.12.002

Cited by 27 publications

(19 citation statements)

References 26 publications

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“…e). The obtained results are in agreement with the literature . It is clear that BSA has an impact on both, activity and stability of HRP–CLEAs (Tab.…”

Section: Resultssupporting

confidence: 91%

“…In a study conducted by Tandjaui et al, similar results were obtained. Namely, for the CLEAs resulting from the white turnip ( Brassica rapa ) fresh extract peroxidase cross‐linking, the optimal concentration of glutaraldehyde was also 2% . For the subsequent experiments, in the immobilization mixture 2% of glutaraldehyde concentration was maintained.…”

Section: Resultsmentioning

confidence: 99%

“…The samples of the filtrate and the enzyme initial solutions used for CLEAs production (offered enzyme) together with the washing solutions, were taken for both protein and activity analysis. Activity recovery and aggregation yield were calculated using the following equations :

Activity recovery false(% false) = \frac{Total activity of HRP - CLEAs}{Total activity of HRP used for CLEA production} \times 100

Aggregation yield false(% false) = true[1 - \frac{Protein content in the residual solution (mg)}{Offered protein amount (mg)} true] \times 100

…”

Section: Methodsmentioning

confidence: 99%

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Removal of Anthraquinone Dye by Cross‐Linked Enzyme Aggregates From Fresh Horseradish Extract

Šekuljica

Prlainović

Jakovetić

et al. 2016

CLEAN Soil Air Water

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Horseradish roots (Armoracia rusticana) fresh extract was the source of unpurified horseradish peroxidase (HRP) which was used for the immobilization in a form of cross-linked enzyme aggregates (CLEAs). Among three precipitants used, acetone appeared to be the best choice for the precipitation. Acetone with 2% of glutaraldehyde gave the recovery of 30.3% of the enzyme initial activity. Horseradish peroxidase immobilized in a form of cross-linked enzyme aggregates (HRP-CLEAs) showed remarkable efficiency in Acid Violet 109 (AV 109) oxidation. Under optimal conditions (pH 4, dye concentration 30 mg L À1 , hydrogen peroxide concentration 0.1 mM), 72.4 and 88.9% of dye was oxidized using HRP-CLEAs in a batch and packed bed reactor, respectively. Higher stability was apparent in the packed bed reactor compared to the batch reactor where the mechanical instability of aggregates under constant agitation has been proved. After seven consecutive cycles the retained activity was 60 and 20% in the recycled packed bed and batch reactor, respectively. The feasibility of HRP-CLEAs application in dye oxidation was confirmed by significant dye oxidation percentage and reduction of the toxicity in the samples collected after enzymatic treatment in the recycled packed bed reactor, using brine shrimp, Artemia salina.

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“…e). The obtained results are in agreement with the literature . It is clear that BSA has an impact on both, activity and stability of HRP–CLEAs (Tab.…”

Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Activity recovery false(% false) = \frac{Total activity of HRP - CLEAs}{Total activity of HRP used for CLEA production} \times 100

Aggregation yield false(% false) = true[1 - \frac{Protein content in the residual solution (mg)}{Offered protein amount (mg)} true] \times 100

…”

Section: Methodsmentioning

confidence: 99%

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Removal of Anthraquinone Dye by Cross‐Linked Enzyme Aggregates From Fresh Horseradish Extract

Šekuljica

Prlainović

Jakovetić

et al. 2016

CLEAN Soil Air Water

View full text Add to dashboard Cite

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“…When acetone was utilized as the precipitant, a 28% activity recovery and 78% aggregation yield of CLEAs were obtained. After incubating at 70 • C for 15 min, the CLEAs precipitated with acetone remained about 37% activity while the free counterpart was found totally inactivated [75]. Compared with acetone and t-butanol, dimethoxyethane was found to be the best precipitant for the preparation of combi-CLEAs including lipase, α-amylase, and phospholipase A 2 .…”

Section: The Precipitantsmentioning

confidence: 95%

Combined Cross-Linked Enzyme Aggregates as Biocatalysts

Wang

et al. 2018

Catalysts

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Enzymes are efficient biocatalysts providing an important tool in many industrial biocatalytic processes. Currently, the immobilized enzymes prepared by the cross-linked enzyme aggregates (CLEAs) have drawn much attention due to their simple preparation and high catalytic efficiency. Combined cross-linked enzyme aggregates (combi-CLEAs) including multiple enzymes have significant advantages for practical applications. In this review, the conditions or factors for the preparation of combi-CLEAs such as the proportion of enzymes, the type of cross-linker, and coupling temperature were discussed based on the reaction mechanism. The recent applications of combi-CLEAs were also reviewed.

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“…Then, the changes in the absorbance at 470 nm were monitored for 5 min. The PA was expressed as U g biomass À1 indicating the amount of peroxidase produced per unit of biomass, which oxidized 1 mmol of substrate per min at 25 C. 30…”

Section: Methodsmentioning

confidence: 99%

Enhanced biodegradation of phenol in a novel cyclic activated sludge integrated with a rotating bed bioreactor in anoxic and peroxidase-mediated conditions

2018

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Preparation and characterization of cross-linked enzyme aggregates (CLEAs) of Brassica rapa peroxidase

Cited by 27 publications

References 26 publications

Removal of Anthraquinone Dye by Cross‐Linked Enzyme Aggregates From Fresh Horseradish Extract

Removal of Anthraquinone Dye by Cross‐Linked Enzyme Aggregates From Fresh Horseradish Extract

Combined Cross-Linked Enzyme Aggregates as Biocatalysts

Enhanced biodegradation of phenol in a novel cyclic activated sludge integrated with a rotating bed bioreactor in anoxic and peroxidase-mediated conditions

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