Preparation and characterization of chia seed protein isolate–chia seed gum complex coacervates

Microencapsulation of chia seed oil using chia seed protein isolate⿿chia seed gum complex coacervates

“…The deoiled chia seed flour (meal) was used for the extraction of gum and protein. Chia seed gum (CSG) and chia seed protein isolate (CPI) were extracted and purified in the laboratory according to the method reported in our previous studies with some modification [15,16,17]. Briefly, 100 g of defatted chia seed flour sample was mixed with 2 L of deionized water with constant stirring for 2 h. The mucilage dissolved in water and the insoluble matters were separated by centrifugation (Allegra 64R Centrifuge, Beckman Coulter Inc., USA) at 10,000 × g for 30 min.…”

“…Chia seed gum (CSG) is an anionic polysaccharide which remains negatively charged from pH 2.0-12.0, while chia seed protein isolate (CPI) becomes positively charged below its isoelectric point (pI= 3.0) [15,16]. Under suitable conditions, these oppositely charged polymers in aqueous solutions interact with each other forming complex coacervates.…”

Physicochemical and functional properties of protein isolate produced from Australian chia seeds

“…Chia seed protein isolate (CPI) was extracted and purified from defatted chia seed flour according to the previously reported method (Timilsena et al, 2016b) with some modification. In brief, 200 g chia seeds were finely ground using a coffee grinder and the oil was extracted using Soxhlet extraction method.…”

“…The solubility of SDCPI was higher than those of FDCPI and VDCPI within the pH range studied. This can be attributed to the lower degree of denaturation of CPI during spray drying as the outlet temperature (80 o C) was lower than the denaturation temperature(T d = 97.2 o C) of CPI (Timilsena et al, 2016b). The spray drying process is very fast and the residence time of CPI inside the drying chamber was very short (6 s in laboratory scale spray dryer and 17 s in the pilot scale spray dryer) (Schmitz-Schug et al, 2013).…”

“…Once the oil is extracted from chia seeds, substantial quantity of protein-rich meal is left behind as the by-product. Chia seed meal can be a valuable source of protein, soluble and insoluble fibres and anti-oxidants as it is comprised of 17-24% protein, 18-30% fibres, 25-40% oil and 5-6% of mucilaginous gum (Sandoval-Oliveros & Paredes-Lopez, 2013;Timilsena et al, 2016b). Current total world production of chia seed is about 30,000 tons per annum (Daniells, 2013).…”