Preparation and Characterization of Callus Extract from Pyrus pyrifolia and Investigation of Its Effects on Skin Regeneration

Abstract

In the present study, an aqueous extract was prepared using calli from the in vitro-derived leaves of Pyrus pyrifolia cultured in Murashige and Skoog medium containing picloram for a plant growth regulator. The major biological components in the callus extract were identified as uridine (1), adenosine (2), and guanosine (3). In terms of the antioxidant activity, at 300 µg/mL, the extract exhibited free radical scavenging activity of 76.9% ± 2.88% in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, comparable to that of 44 µg/mL ascorbic acid (82.5% ± 3.63%). In addition, the IC 50 values for inhibition of advanced glycation end product formation from collagen and elastin were 602 ± 2.72 and 3037 ± 102.5 µg/mL, respectively. The extract significantly promoted keratinocyte and fibroblast cell proliferation in a dose-dependent manner. Moreover, fibroblasts treated with 1.36 µg/mL extract exhibited a 1.60-fold increase in procollagen type I C-peptide level compared to controls. The in vitro wound recovery rates of keratinocytes and fibroblasts were also 75% and 38% greater, respectively, than those of serum-free controls at 9 and 36 h after extract treatment (1.36 µg/mL). Additionally, the extract flux across the human epidermis increased by 1598% after its incorporation into elastic nanoliposomes (NLs). Therefore, elastic NLs loaded with Pyrus pyrifolia callus extract have potential use as skin rejuvenators and antiaging ingredients in cosmetic formulations.

Effects of Tiarella polyphylla D. Don Callus Extract on Photoaging in Human Foreskin Fibroblasts Hs68 Cells

“…The plant tissue and cell culture techniques were used for overcoming barriers and for uniformly controlled production of plant-derived bioactive components. 20 The awareness of cosmetic products of botanical origin on skin has increased in the most recent years among consumers. 21 Because of people generally believe that such products are safety, this practice continues to trend upwards.…”

“…The plant tissue and cell culture techniques were used for overcoming barriers and for uniformly controlled production of plant-derived bioactive components. 20…”

Plant In Vitro Systems as a Sustainable Source of Active Ingredients for Cosmeceutical Application

“…The incorporation of P. pyrifolia aqueous extract in nanoliposomes increased its flux across the human epidermis by ca. 16 times and facilitated the in vitro wound recovery rates of keratinocytes and fibroblasts [84]. Another successful approach to increase the bioavailability and permeability of substances with low lipophilicities is to obtain their derivatives with increased lipophilic properties or their inclusion into liposomes or lipogels.…”

Roślinne Komórki Macierzyste I Ich Zastosowanie W Kosmetologii I Medycynie Regeneracyjnej

“…Wykazano, że ekstrakt n-heksanowy w największym stopniu pobudzał migrację komórek, co ma istotne znaczenie w procesie zarastania rany. Naukowcy sugerują, że materiały lipidowe zawarte w tym ekstrakcie mogą stymulować ekspresję czynników wzrostu wewnątrz rany [39].…”