Preparation and Characterization of Antiserum to Purified Gonadotrophins From Rat Pituitary Glands

Lunenfeld, Bruno; Eshkol, Aliza; Baldratti, G; Suchowsky, G. K.

doi:10.1530/acta.0.0540311

Cited by 9 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Littermate newborn ICR Swiss-Albino mice were used in all experiments. One group was injected with 0-1 ml antiserum to rat gonadotrophins (aRG) (Lunenfeld, Eshkol, Baldratti & Suchowsky, 1967) daily up to the age of 10 days. A second group received daily injections of aRG plus biologically pure human FSH devoid of LH activity , while the control animals were given 0-1 ml normal rabbit serum daily.…”

Section: Methodsmentioning

confidence: 99%

“…We have carried out ultrastructural analyses in an attempt to determine to what extent substitution with FSH could prevent the developmental changes observed following deprivation of both gonadotrophic hormones (LH plus FSH) in mice. Deprivation was achieved by neutralizing the endogenous gonadotrophins with rabbit anti-rat gonadotro¬ phins (Lunenfeld, Eshkol, Baldratti & Suchowsky, 1967).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultrastructural Changes in the Ovaries of Infant Mice Deprived of Endogenous Gonadotrophins and After Substitution With FSH

Hardy¹,

Danon²,

Eshkol³

et al. 1974

Reproduction

Self Cite

View full text Add to dashboard Cite

The ultrastructure of ovaries of infant mice deprived of gonadotrophins was compared to the normal developmental pattern and to that of animals deprived of endogenous gonadotrophins but receiving FSH. In the absence of circulating gonadotrophins, structural changes were observed ; granulosa-cell nuclei were irregularly shaped rather than round, and the spaces between the granulosa cells disappeared as did the spaces between the granulosa cells and the oocyte. The most striking effect was observed in the zona pellucida, the width of which diminished and became irregular. The number of cell processes within the zona pellucida was markedly reduced. These changes are tentatively attributed to alteration in the structure of the granulosa cell. Substitution with FSH partly prevented these changes, indicating that FSH has a r\l=o^\leto play in the preservation of the integrity of the granulosa-cell membrane.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ultrastructural Changes in the Ovaries of Infant Mice Deprived of Endogenous Gonadotrophins and After Substitution With FSH

Hardy¹,

Danon²,

Eshkol³

et al. 1974

Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…Rat LH assay reagents obtained from the NIAMDD, NIH Rat Pituitary Hormone Distribution Program, Bethesda, Maryland, U.S.A., were used and LH values are expressed as ng NIAMDD-rat-LH-RP-1/ml. A radioimmunoassay for rat LH was used as Lunenfeld, Eshkol, Baldratti & Suchowsky (1967) had previously precipitated mouse LH by using a rat anti-LH antibody. A preliminary trial with the NIAMDD rat reagents using serum from male mice of the TO strain showed good cross-reaction with the antibody.…”

Section: Gonadotrophin Assaymentioning

confidence: 99%

Hormone Replacement Therapy in the Menopause: A Suitable Animal Model

Papadaki¹,

Beilby²,

Chowaniec³

et al. 1979

Journal of Endocrinology

View full text Add to dashboard Cite

Female CBA mice, aged 11 months, were treated cyclically with oral ethynyl oestradiol or oestrone sulphate for 3 months. The ovaries of all animals appeared to be atrophied. Target tissues throughout the genital tract showed a response to both oestrogens. Electron microscopy of both the endometrium and the urothelium demonstrated morphological changes characteristic of increased cellular metabolic activity in the treated mice. Endometrial hyperplasia developed in both treatment groups but more pronounced epithelial changes occurred with oestrone sulphate. This hyperplasia was accompanied by a doubling in the number of uterine cytoplasmic oestrogen receptors. A 50% fall in serum levels of luteinizing hormone in the treated mice revealed that the hypothalamic-pituitary system was still intact. Both oestrogens improved skeletal balance by changes in cortical-endosteal bone remodelling. The results suggest that the CBA strain of mouse is a suitable model for the study of the human climacteric and its response to hormone replacement therapy.

show abstract

“…Antiserum to sheep gonadotropins was inappropriate due to its broad spectrum of cross reactions (table I). Antiserum to bovine gonadotropins also exhibits reac tivity with gonadotropins of many species including man [42], Antiserum to rat pituitary gonadotropins has been prepared and charac terized by Lunenfeld et al [28]. This antiserum neutralized the biological effects of rat pituitary FSF1 and LH and cross-reacted with endogenous mouse gondadotropins.…”

Section: Effects O F Gonadotropic Deprivation and Specific Gonadotropmentioning

confidence: 99%

Biological Effects of Antibodies to Gonadotropins With colour plate I

Eshkol¹,

Lunenfeld²

1971

Gynecol Obstet Invest

Self Cite

View full text Add to dashboard Cite

Some of the contradictory postulates regarding gonadotropin action in infancy were reexamined. For the deprivation of newborn animals of endogenous gonadotropins, antiserum neutralizing theese hormones was administered from birth on. Using this approach it was elucidated that, even during the first week of life, gonadotropins stimulate development of ovarian somatic elements and are indispensable for this process. In the absence of the stimulatory action of endogenous gonadotropins, the normal ovarian development taking place during the first and second week of life was markedly altered and retarded. This was mainly evident from the reduction in the number of follicles which developed, the change in the appearance and ultrastructure of granulosa cells, the deficiency of thecal and of vascular development. That the observed alterations were due specifically to gonadotropin deprivation was proven by the fact that these alterations were prevented by the administration of human gonadotropins to mice deprived of endogenous gonadotropins. Substitution with human gonadotropins permitted the elucidation of the respective role of follicle-stimulating hormone (FSH) and FSH plus luteinizing hormone (LH) in the development of the infantile ovary. This was achieved by the use of HMG and of a biologically ‘pure FSH’ which was obtained by the neutralization of the LH activity present in HMG. FSH was found to be primarily responsible for the stimulation of granulosa cell proliferation, organization and structure. FSH plus LH, in addition to the FSH effects, initiated secretory activity of granulosa cells, increase in intrafollicular spaces, antrum formation, enrichment and maintenance of the theca layer and development of the vascular system. At the age of 14 days, the administration of HMG to gonadotropin-deprived animals even enhanced some of the developmental phases beyond that observed in ovaries of control animals at the same age. Oocyte growth seems to be independent of gonadotropins since it was similar in all experimental groups. The functional integrity of the growing oocytes in gonadotropin-deprived animals could not be assessed in this study.

show abstract

Preparation and Characterization of Antiserum to Purified Gonadotrophins From Rat Pituitary Glands

Cited by 9 publications

References 0 publications

Ultrastructural Changes in the Ovaries of Infant Mice Deprived of Endogenous Gonadotrophins and After Substitution With FSH

Ultrastructural Changes in the Ovaries of Infant Mice Deprived of Endogenous Gonadotrophins and After Substitution With FSH

Hormone Replacement Therapy in the Menopause: A Suitable Animal Model

Biological Effects of Antibodies to Gonadotropins With colour plate I

Contact Info

Product

Resources

About