Preparation and characterization of antibodies to the λ chain variable region (V_λ) of mouse immunoglobulins

Abstract

The variable portion (V,) of the mouse myeloma protein MOPC-315 (a, h 2 ) was obtained from its Fv fragment and was used to immunize rabbits. Anti-VL antibodies were found to be specific to the V, region of protein 3 15 and to precipitate V,, Fv or light chain but not Fab' or intact M315. Anti-VL 315 precipitates also the light chain of MOPC-I04E (p, xl) but not the intact protein. Intact immunoglobulins (Ig) containing h chains, although they d o not precipitate with anti-VL, inhibit the binding of anti-V, t o VL in the radioimmunoassay. Radioimmunoassay inhibition studies demonstrated that VAl and Vhz cross-react extensively and that anti-VL3 15 can be used as a general anti-Vh reagent t o detect molecules containing chains in mouse serum. Analysis of sera from several mouse strains indicated that Vh-containing molecules are present in approximately 3 % of the Ig population, whereas the SJL strain has no detectable VA-bearing molecules. In some of the antisera, anti-V,3 15 slightly cross-reacted with V,-bearing molecules. Anti-VL31 5 antibodies are not anti-idiotypes since they react with myeloma proteins M315 (anti-2,4 dinitrophenyl MOPC-104E (anti-dextran) and HOPC-1 (specificity unknown) and with most s,erum Ig containing h chains. I n view of the similarity of mouse 1 chain sequences, it is suggested that most anti-VL315 is "anti-framework'' antibody.

Correlated expression of VH framework and VHidiotypic determinants on T helper cells and on functionally undefined T cells binding group A streptococcal carbohydrate

“…This was expected because it has been shown in serological experiments that our anti-V, antibody reacts very poorly with intact Ig molecules [17]. The situation is different for our anti-Vh reagent which reacts well with Ig molecules bearing h chains [18] so that the lack of inhibition in this case suggests that an insignificant number of A-CHO-binding B cells bear A chains or, alternatively, that anti-Vh reacts with the Ig molecule at a site where it does not interfere with the combining site. The former alternative is in agreement with previous observations that the majority of anti-A-CHO antibodies of strain A/J possess x chains [22].…”

Selective participation of immunoglobulin V region and major histocompatibility complex products in antigen binding by T cells

“…In this study, it will be shown that VH and VL may be separately involved in antigen binding by cells which belong t o different T cell subclasses. I t will be demonstrated that antigen binding t o Lyt-l+ class cells is specifically inhibited by anti-VH [2], whereas antigen binding to Lyt-2+,3+ cells is inhibited b y anti-VL antibodies [3]. Therefore, we suggest that antigen binding b y Lyt-l+ T cells is affected b y molecules containing the variable region of heavy chain (VH), whereas antigen binding t o Lyt-2+,3+ T cells is affected b y VL-containing molecules.…”

Partial maturation and light chain restriction of Abelson virus‐transformed B cell precursors

“…The transformation and isolation of the ABC-1 cell line has been described previously [8]. The AT1 subline arose as the predominant cell type in one culture.…”