Partial maturation and light chain restriction of Abelson virus‐transformed B cell precursors

Boss, Michael A.; Greaves, Melvyn F.; Teich, Natalie M.

doi:10.1002/eji.1830110214

Cited by 5 publications

(6 citation statements)

References 13 publications

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“…The presence of cytoplasmic Fc chain in a minor proportion of ABC-1 cells has previously been indicated by immunofluorescence studies (21) . Our Southern blot analyses of 14 randomly selected ABC-1 subclones showed only a single recombinedJH region, which is linked to the Cu gene (not shown).…”

Section: Resultsmentioning

confidence: 76%

“…Previous reports have indicated that no ABC-1 cells express cell surface Ig, although low levels of cytoplasmic p chain have been detected within some cells of the ABC-I population (20,21). In addition, reported analyses of the AT 1 subline, which was derived from ABC-1, indicated that a small proportion of AT1 cells exhibited positive staining for cytoplasmic a light chain protein (21). ABC-1 cells possess low but detectable levels of terminal deoxynucleotidyl transferase (20), but do not express the surface markers Thy-1 .2, la, TL 1, 2, 3, 4, or peanut agglutinin (20).…”

Section: Methodsmentioning

confidence: 98%

“…The ABC-1 cell line also originated from adult BALB/c mouse bone marrow cells transformed in vitro by A-MuLV (19). Previous reports have indicated that no ABC-1 cells express cell surface Ig, although low levels of cytoplasmic p chain have been detected within some cells of the ABC-I population (20,21). In addition, reported analyses of the AT 1 subline, which was derived from ABC-1, indicated that a small proportion of AT1 cells exhibited positive staining for cytoplasmic a light chain protein (21).…”

Section: Methodsmentioning

confidence: 99%

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Active lambda and kappa antibody gene rearrangement in Abelson murine leukemia virus-transformed pre-B cell lines.

Persiani¹,

Durdik²,

Selsing³

1987

The Journal of Experimental Medicine

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The two Abelson murine leukemia virus (A-MuLV)-transformed cell lines, BM18-4 and ABC-1, undergo immunoglobulin L-chain gene recombination during passage in tissue culture. BM18-4 cells are capable of kappa gene recombination, whereas ABC-1 cells are capable of both kappa and lambda gene recombination. The expression of H chains is apparently not necessary for continuing L chain gene recombination in either of these cells, although H-chain expression may have been involved in the initiation of L-chain gene recombination. All ABC-1 cells that have lambda gene rearrangements also display recombined kappa alleles, supporting the hypothesis that kappa and lambda gene recombination are initiated in an ordered, developmentally regulated manner in maturing B cells. However, analyses of the ABC-1 line indicate that pre-B cells that have initiated lambda gene recombination do not terminate kappa gene rearrangement. The lambda gene recombinations that occur in the ABC-1 cell line indicate that the germline order of lambda gene segments is: 5' ... V lambda 2 ... J lambda 2C lambda 2-J lambda 4C lambda 4 ... V lambda 1 ... J lambda 3C lambda 3-J lambda 1C lambda 1 ... 3'. In addition, the frequencies of lambda 1, lambda 2, and lambda 3 gene recombinations among ABC-1 cells are quite different than the frequencies of B cells producing lambda 1, lambda 2, and lambda 3 L-chains in the mouse. RS DNA recombinations also occur in the BM18-4 and ABC-1 cell lines, supporting the notion that Ig gene recombinases are involved in RS rearrangement. Recombined RS segments are infrequent among BM 18-4 cells but common among ABC-1 cells, suggesting that RS recombinational events often occur in maturing pre-B cells just before initiation of lambda gene rearrangements. This developmental timing is consistent with the hypothesis that RS recombination may be involved in the initiation of lambda gene assembly.

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Section: Resultsmentioning

confidence: 76%

Section: Methodsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

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Active lambda and kappa antibody gene rearrangement in Abelson murine leukemia virus-transformed pre-B cell lines.

Persiani¹,

Durdik²,

Selsing³

1987

The Journal of Experimental Medicine

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“…Curiously, lymphoid malignancies have not been very extensively studied from this viewpoint which is perhaps surprising since this might provide insight into factors regulating lymphocyte differentiation, subset and clonal diversification. Minimal further maturation within the B-cell lineage is inducible in Abelson-virustransformed B-cell precursors (Baltimore et al, 1979;Boss et al, 1981) and it is reported that phorbol ester increases cytoplasmic immunoglobulin and induces morphological maturation towards plasma cells in Bchronic lymphocytic leukaemia cells in humans (Totterman et al, 1980). Similarly, phorbol ester was reported to increase sheep erythrocyte rosette formation and decrease terminal deoxynucleotidyl transferase activity in a thymic leukaemia cell line, MOLT-3 (Nagasawa and Mak, 1980); these two alterations are considered to reflect induction of T-cell differentiation.…”

mentioning

confidence: 99%

Modulation of T leukaemic cell phenotype with phorbol ester

Delia

Greaves

Newman

et al. 1982

Intl Journal of Cancer

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A panel of monoclonal antibodies and other markers (e.g., terminal deoxynucleotidyl transferase, sheep erythrocyte rosettes, peanut agglutinin) have been used in conjunction with flow cytometry and biochemical analysis to monitor the induction of maturation in human thymic (T) leukaemic cell lines by phorbol ester (TPA). Seven cell lines underwent multiple phenotypic alterations in response to TPA but were unresponsive to synthetic thymic hormones (TP5, FTS) or to other compounds (e.g. DMSO, retinoic acid) which induce maturation in other types of leukaemia. The changes parallel those observed in normal T-cell differentiation and partly reflect alterations in glycosyl transferase activity, altered synthesis of proteins and regulation of cell surface receptors (for transferrin) associated with rapid growth and metabolism. These studies further illustrate the reversibility of maturation arrest in human leukaemia and provide support for the view that leukaemia may involve regulatory defects in the coupling of proliferation and maturation. Induction of promotion of terminal differentiation in leukaemic equivalents of T-cell precursors may provide a convenient system for the study of biochemical and molecular events involved in T-cell development and diversification.

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“…The lines were maintained in tissue culture in RPMI 1640 medium (GIBCO, Grand Island, NY) with 50 pM 2-mercaptoethanol supplemented with 10% heat-inactivated fetal calf serum. The BM18-4 cell line (12) has previously been shown to undergo continual K gene recombination in culture (5) and the ABC-1 cell line (13,14) has been shown to undergo both K and X gene recombination during propagation in culture (5). The 38B9 cell line has been shown to be capable of site-specific assembly of VH gene segments during growth in culture (2,15).…”

Section: Methodsmentioning

confidence: 99%

DNase I sensitivity of immunoglobulin light chain genes in Abelson murine leukemia virus transformed pre-B cell lines

Persiani

Selsing

1989

Nucl Acids Res

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We have used Abelson murine leukemia virus (A-MuLV) transformed pre-B cell lines to test the hypothesis that the rearrangement potential of a developing B-lymphocyte is dependent on an "opening" of the chromatin structure surrounding immunoglobulin (Ig) genes, thus allowing accessibility to an Ig gene recombinase.The chromatin structures surrounding heavy (H), kappa (K), and lambda (X) chain constant-region genes were assessed by DNase I sensitivity in A-MuLV transformed cell lines capable of H, K or X gene rearrangement. Our results indicate that DNase I-sensitive chromatin structures of these Ig constant-region genes correlate closely with the ability of the genes to undergo recombination.We also find that the chromatin structure of an Ig constantregion locus becomes DNase I sensitive before any DNA rearrangement events occur.

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Partial maturation and light chain restriction of Abelson virus‐transformed B cell precursors

Cited by 5 publications

References 13 publications

Active lambda and kappa antibody gene rearrangement in Abelson murine leukemia virus-transformed pre-B cell lines.

Active lambda and kappa antibody gene rearrangement in Abelson murine leukemia virus-transformed pre-B cell lines.

Modulation of T leukaemic cell phenotype with phorbol ester

DNase I sensitivity of immunoglobulin light chain genes in Abelson murine leukemia virus transformed pre-B cell lines

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