Preparation and characterization of alginate/gelatin blend films for cardiac tissue engineering

Rosellini, Elisabetta; Cristallini, Caterina; Barbani, Niccoletta; Vozzi, Giovanni; Giusti, P.

doi:10.1002/jbm.a.32216

Cited by 166 publications

(141 citation statements)

References 19 publications

Supporting

Mentioning

131

Contrasting

Order By: Relevance

“…Rosellini et al [23] showed that in vitro degradation behavior at 37 ºC of gelatin crosslinked with GA films in PBS is quite similar to that obtained in PBS collagenase solution. Electrospun cross-linked samples were immersed in PBS solution and the extent of hydrolytic degradation was determined (Figure 2).…”

Section: Hydrolytic Degradationmentioning

confidence: 72%

See 1 more Smart Citation

Thermal and hydrolytic degradation of electrospun fish gelatin membranes

et al. 2013

View full text Add to dashboard Cite

The thermal and hydrolytic degradation of electrospun gelatin membranes cross-linked with glutaraldehyde in vapor phase has been studied. In vitro degradation of gelatin membranes was evaluated in phosphate buffer saline solution at 37 ºC. After 15 days under these conditions, a weight loss of 68 % was observed, attributed to solvation and depolymerization of the main polymeric chains. Thermal degradation kinetics of the gelatin raw material and as-spun electrospun membranes showed that the electrospinning processing conditions do not influence polymer degradation. However, for cross-linked samples a decrease in the activation energy was observed, associated with the effect of glutaraldehyde cross-linking reaction in the inter-and intra-molecular hydrogen bonds of the protein. It is also shown that the electrospinning process does not affect the formation of the helical structure of gelatin chains. Polymer Testing 32 (5), 995http://dx

show abstract

Section: Hydrolytic Degradationmentioning

confidence: 72%

“…Rosellini et al [23] studied the effect of the presence of collagenase in the phosphate buffer saline (PBS) solution during in vitro degradation of gelatin scaffolds cross-linked with GA, and found that a mainly hydrolytic degradation process occurs due to solvation and depolymerization of the polymeric chains [24].…”

mentioning

confidence: 99%

Thermal and hydrolytic degradation of electrospun fish gelatin membranes

et al. 2013

View full text Add to dashboard Cite

show abstract

“…The highly hydrated network structure permits the exchange of gases and nutrients and makes them an attractive option for the 55 formation of "inks" for bioprinting. Blending of hydrogels provides an opportunity by which properties specific to each respective hydrogel component can be combined to tailor the overall hydrogel towards facilitating specific requirements 8,9 . Alginates (Alg) are naturally occurring polysaccharides 60 isolated from brown algae with linear blocks of (1, 4)-linked β-Dmannuronate (M) acid and α-L-guluronic acid (G) residues 10,11 .…”

mentioning

confidence: 99%

“…Alginate-gelatin (Alg-Gel) blends have been reported as potential drug delivery carriers 8,9 , enzyme immobilisation beads 5 22 , wound dressing fibres 23 , and sponges for tissue matrices 24 . Among the studies related to bioprinting, Yan and co-workers [25][26][27] have attempted to print 3D scaffolds from alginate-gelatin blends.…”

mentioning

confidence: 99%

Bio-ink properties and printability for extrusion printing living cells

et al. 2013

View full text Add to dashboard Cite

Additive biofabrication (3D bioprinting) makes it possible to create scaffolds with precise geometries, control over pore interconnectivity and architectures that are not possible with conventional techniques. Inclusion of cells within the ink to form a "bio-ink" presents the potential to print 3D structures that can be implanted into damaged/diseased tissue to promote highly controlled cell-based regeneration and repair. The properties of an 'ink' are defined by its formulation and critically influence the delivery and integrity of structure formed. Importantly, the ink properties need to conform to biological requirements necessary for the cell system that they are intended to support and it is often challenging to find conditions for printing that facilitate this critical aspect of tissue bioengineering. In this study, alginate (Alg) was selected as the major component of the 'bio-ink' formulations for extrusion printing of cells. The rheological properties of alginate-gelatin (AlgGel) blends were compared with pre-crosslinked alginate and alginate solution to establish their printability whilst maintaining their ability to support optimal cell growth. Pre-crosslinked alginate on its own was liquidlike during printing. However, by controlling the temperature, Alg-Gel formulations had higher viscosity, storage modulus and consistency which facilitated higher print resolution/precision. Compression and indentation testing were used to examine the mechanical properties of alginate compared to Alg-Gel. Both types of gels yielded similar results with modulus increasing with alginate concentration. Decay in mechanical properties over time suggests that Alg-Gel slowly degrades in cell culture media with more than 60% decrease in initial modulus over 7 days. The viability of primary myoblasts delivered as a myoblast/Alg-Gel bio-ink was not affected by the printing process, indicating that the Alg-Gel matrix provides a potential means to print 3D constructs that may find application in myoregenerative applications. control over pore interconnectivity and architectures that are not possible with conventional techniques. Inclusion of cells within the ink to form a "bio-ink" presents the potential to print 3D structures that can be implanted into damaged/diseased tissue to promote highly controlled cell-based regeneration and repair. The properties of an 'ink' are defined by its formulation and critically influence the delivery and integrity of structure formed. Importantly, the ink properties need to conform to biological requirements 10 necessary for the cell system that they are intended to support and it is often challenging to find conditions for printing that facilitate this critical aspect of tissue bioengineering. In this study, alginate (Alg) was selected as the major component of the 'bio-ink' formulations for extrusion printing of cells. The rheological properties of alginate-gelatin (Alg-Gel) blends were compared with pre-crosslinked alginate and alginate solution to establish their printability whil...

show abstract

“…HA is gaining popularity as a biomaterial for tissue engineering and tissue regeneration due to its high biocompatibility and low immunogenicity (Zheng et al, 2004;Leach et al, 2015) as well as its ability to promote cell migration (Yoo et al, 2005). Previously, it has been shown that rate of cell proliferation and cardiomyogenic differentiation of C2C12 myoblasts was significantly increased with a film like scaffold, which was produced by mixing alginate and gelatin (Rosellini et al, 2009), and films including gelatin in high proportions were proposed as ideal scaffolds. Considering the previous findings and gelatin's effect in induction of cardiomyogenic differentiation, as well as its positive effects on cell attachment and expansion, gelatin concentration in the HA/G plasma gels was kept higher than the HA concentration in the present study.…”

Section: Discussionmentioning

confidence: 99%

Cardiomyogenic differentiation potential of human lipoaspirate-derivedstem cells on hyaluronic acid/gelatin plasma gels

Göv¹,

Kenar²,

Halbutoğulları³

et al. 2016

Turk J Biol

View full text Add to dashboard Cite

Cardiomyogenic differentiation from mesenchymal stem cells has emerged as a novel approach for repair of damaged myocardium. Cell transplantation through direct cell injection is not an optimal method due to the lack of cell-extracellular matrix interactions. In the present study, differentiation potential of human adipose-derived stem cells (ASCs) to cardiomyocytes has been investigated by growing them on hyaluronic acid/gelatin (HA/G) plasma gels and coverslips and supplementing the growth medium with chemical modifiers (activin-a, BMP-4, insulin, valproic acid, and 5-azacytidine) in various combinations. The HA/G plasma gels were produced from human blood plasma-derived fibrinogen, gelatin, and human umbilical cord-derived hyaluronic acid. A networkbased approach was employed to select marker genes for cardiomyogenic differentiation, and the expression levels of three markers (GATA4, TBX5, and cTnI) were followed by RT-qPCR to investigate the cardiomyogenic differentiation potential of ASCs. Results indicated that each combination of chemical modifiers led to different expression levels in the aforementioned cardiac markers, and this was material-dependent, too. The cardiac gene expression on HA/G plasma gels in the presence of activin-a + BMP-4 or insulin + valproic acid was more pronounced than in the presence of 5-azacytidine only, and scaffold and chemical modifier combinations were crucial for cardiomyogenic differentiation.

show abstract

Preparation and characterization of alginate/gelatin blend films for cardiac tissue engineering

Cited by 166 publications

References 19 publications

Thermal and hydrolytic degradation of electrospun fish gelatin membranes

Thermal and hydrolytic degradation of electrospun fish gelatin membranes

Bio-ink properties and printability for extrusion printing living cells

Cardiomyogenic differentiation potential of human lipoaspirate-derivedstem cells on hyaluronic acid/gelatin plasma gels

Contact Info

Product

Resources

About