Cardiomyogenic differentiation potential of human lipoaspirate-derivedstem cells on hyaluronic acid/gelatin plasma gels

Göv, Esra; Kenar, Halime; Halbutoğulları, Zehra Seda; Arğa, Kazım Yalçın; Karaöz, Erdal

doi:10.3906/biy-1504-7

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…Recently, it has been well recognized that cell behaviors, including the differentiation of stem cells, can be influenced by the mechanical properties of the 3D microenvironment [18,19]. Scaffolds consisting of both gelatin and hyaluronic acid also have been prepared for culturing varieties of cells such as vascular endothelial cells [20], myoblasts [21], osteoblasts [22], and stem cells [8,23,24,25].…”

Section: Introductionmentioning

confidence: 99%

Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors

2019

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Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields. In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering. In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels. Control of the content enabled gelation in less than 20 s, and also enabled hydrogels to be obtained with 0.5–1.2 kPa Young’s modulus. Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid. Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes. These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors

2019

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“…The use of hyaluronic acid has been shown to be largely dependent on its molecular weight, and several kinds of literature have reported its successful use in cardiac tissue repair ( Bonafè et al, 2014 ; Yoon et al, 2009 ; Yang et al, 2010 ; Göv et al, 2016 ).…”

Section: Congenital Heart Disease: Types Malformations Presentationmentioning

confidence: 99%

Recent advances and challenges on application of tissue engineering for treatment of congenital heart disease

Mantakaki¹,

Fakoya

Sharifpanah

2018

PeerJ

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Congenital heart disease (CHD) affects a considerable number of children and adults worldwide. This implicates not only developmental disorders, high mortality, and reduced quality of life but also, high costs for the healthcare systems. CHD refers to a variety of heart and vascular malformations which could be very challenging to reconstruct the malformed region surgically, especially when the patient is an infant or a child. Advanced technology and research have offered a better mechanistic insight on the impact of CHD in the heart and vascular system of infants, children, and adults and identified potential therapeutic solutions. Many artificial materials and devices have been used for cardiovascular surgery. Surgeons and the medical industry created and evolved the ball valves to the carbon-based leaflet valves and introduced bioprosthesis as an alternative. However, with research further progressing, contracting tissue has been developed in laboratories and tissue engineering (TE) could represent a revolutionary answer for CHD surgery. Development of engineered tissue for cardiac and aortic reconstruction for developing bodies of infants and children can be very challenging. Nevertheless, using acellular scaffolds, allograft, xenografts, and autografts is already very common. Seeding of cells on surface and within scaffold is a key challenging factor for use of the above. The use of different types of stem cells has been investigated and proven to be suitable for tissue engineering. They are the most promising source of cells for heart reconstruction in a developing body, even for adults. Some stem cell types are more effective than others, with some disadvantages which may be eliminated in the future.

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“…It was noted that the CD44 surface markers influenced the differentiation [ 65 ]. Combining HA and gelatin together with other chemical modifiers like activin-a, BMP-4, insulin, valproic acid, and 5-azacytidine in various combinations leads to the differentiation of human ADSC to CM with the expression of GATA4, TBX5, and cTnI [ 66 ].…”

Section: Biomaterials For Cardiac Regenerationmentioning

confidence: 99%

Current Trends in Biomaterial Utilization for Cardiopulmonary System Regeneration

Fakoya

Otohinoyi

Yusuf

2018

Stem Cells International

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The cardiopulmonary system is made up of the heart and the lungs, with the core function of one complementing the other. The unimpeded and optimal cycling of blood between these two systems is pivotal to the overall function of the entire human body. Although the function of the cardiopulmonary system appears uncomplicated, the tissues that make up this system are undoubtedly complex. Hence, damage to this system is undesirable as its capacity to self-regenerate is quite limited. The surge in the incidence and prevalence of cardiopulmonary diseases has reached a critical state for a top-notch response as it currently tops the mortality table. Several therapies currently being utilized can only sustain chronically ailing patients for a short period while they are awaiting a possible transplant, which is also not devoid of complications. Regenerative therapeutic techniques now appear to be a potential approach to solve this conundrum posed by these poorly self-regenerating tissues. Stem cell therapy alone appears not to be sufficient to provide the desired tissue regeneration and hence the drive for biomaterials that can support its transplantation and translation, providing not only physical support to seeded cells but also chemical and physiological cues to the cells to facilitate tissue regeneration. The cardiac and pulmonary systems, although literarily seen as just being functionally and spatially cooperative, as shown by their diverse and dissimilar adult cellular and tissue composition has been proven to share some common embryological codevelopment. However, necessitating their consideration for separate review is the immense adult architectural difference in these systems. This review also looks at details on new biological and synthetic biomaterials, tissue engineering, nanotechnology, and organ decellularization for cardiopulmonary regenerative therapies.

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Cardiomyogenic differentiation potential of human lipoaspirate-derivedstem cells on hyaluronic acid/gelatin plasma gels

Cited by 4 publications

References 48 publications

Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors

Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors

Recent advances and challenges on application of tissue engineering for treatment of congenital heart disease

Current Trends in Biomaterial Utilization for Cardiopulmonary System Regeneration

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