Preparation and characterization of a novel pyrrole-benzophenone copolymerized silica nanocomposite as a reagent in a visual immunologic-agglutination test

Oppenheimer, Pola Goldberg; Cosnier, Serge; Marks, Robert S.; Regev, Oren

doi:10.1016/j.talanta.2008.01.060

Cited by 9 publications

(4 citation statements)

References 26 publications

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“…BP-modified biointerfaces have also been useful in generating antimicrobial coating for implants and textiles, as well as in immunodiagnostics for detecting and quantifying antigen–antibody binding interactions within biological samples. − Exploiting the optical and conductive properties inherent to glass substrates coated with indium tin oxide (ITO), Konry et al fabricated an immunosensor capable of detecting the hepatitis C virus (HCV) in human blood samples from patients (Figure ). Monolayers presenting BP were electropolymerized on the ITO devices, followed by the BP-mediated photoimmobilization (345 nm) of HCV-E2 envelope protein antigen.…”

Section: Photoimmobilization and Surface Modification By Bpmentioning

confidence: 99%

The Life of Pi Star: Exploring the Exciting and Forbidden Worlds of the Benzophenone Photophore

et al. 2016

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The widespread applications of benzophenone (BP) photochemistry in biological chemistry, bioorganic chemistry, and material science have been prominent in both academic and industrial research. BP photophores have unique photochemical properties: upon n-π* excitation at 365 nm, a biradicaloid triplet state is formed reversibly, which can abstract a hydrogen atom from accessible C-H bonds; the radicals subsequently recombine, creating a stable covalent C-C bond. This light-directed covalent attachment process is exploited in many different ways: (i) binding/contact site mapping of ligand (or protein)-protein interactions; (ii) identification of molecular targets and interactome mapping; (iii) proteome profiling; (iv) bioconjugation and site-directed modification of biopolymers; (v) surface grafting and immobilization. BP photochemistry also has many practical advantages, including low reactivity toward water, stability in ambient light, and the convenient excitation at 365 nm. In addition, several BP-containing building blocks and reagents are commercially available. In this review, we explore the "forbidden" (transitions) and excitation-activated world of photoinduced covalent attachment of BP photophores by touring a colorful palette of recent examples. In this exploration, we will see the pros and cons of using BP photophores, and we hope that both novice and expert photolabelers will enjoy and be inspired by the breadth and depth of possibilities.

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Section: Photoimmobilization and Surface Modification By Bpmentioning

confidence: 99%

The Life of Pi Star: Exploring the Exciting and Forbidden Worlds of the Benzophenone Photophore

et al. 2016

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“…Still, a simplification of these techniques would be a welcome event 7. Therefore, a variety of remarkable probes based on organic molecules,8 polymeric materials,9 biomaterials,10 semiconductor nanocrystals,11 and agglutination materials12 have been developed for Hg 2+ detection over the past decades, which normally test Hg 2+ using optical and electrochemical signals 13. However, most of examples are not well suited for on‐field analysis or rapid screening.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Detection of Mercury Ions Based on Plasmonic Nanoparticles

Du¹,

Jiang²,

Shao³

et al. 2012

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165

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The development of rapid, specific, cost-effective, and robust tools in monitoring Hg(2+) levels in both environmental and biological samples is of utmost importance due to the severe mercury toxicity to humans. A number of techniques exist, but the colorimetric assay, which is reviewed herein, is shown to be a possible tool in monitoring the level of mercury. These assays allow transforming target sensing events into color changes, which have applicable potential for in-the-field application through naked-eye detection. Specifically, plasmonic nanoparticle-based colorimetric assay exhibits a much better propensity for identifying various targets in terms of sensitivity, solubility, and stability compared to commonly used organic chromophores. In this review, recent progress in the development of gold nanoparticle-based colorimetric assays for Hg(2+) is summarized, with a particular emphasis on examples of functionalized gold nanoparticle systems with oligonucleotides, oligopeptides, and functional molecules. Besides highlighting the current design principle for plasmonic nanoparticle-based colorimetric probes, the discussions on challenges and the prospect of next-generation probes for in-the-field applications are also presented.

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“…This approach was also exploited for the elaboration of polypyrrole-benzophenone SiO 2 nanocomposites. This photoactive nanocomposite served for the photografting of cholera toxin B and was used as a reagent in a visual immunologic-agglutination test [104]. …”

Section: Covalent Binding At the Surface Of Electropolymerized Filmsmentioning

confidence: 99%

Biosensors Based on Electropolymerized Films

Cosnier

Holzinger

2010

Electropolymerization

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Preparation and characterization of a novel pyrrole-benzophenone copolymerized silica nanocomposite as a reagent in a visual immunologic-agglutination test

Cited by 9 publications

References 26 publications

The Life of Pi Star: Exploring the Exciting and Forbidden Worlds of the Benzophenone Photophore

The Life of Pi Star: Exploring the Exciting and Forbidden Worlds of the Benzophenone Photophore

Colorimetric Detection of Mercury Ions Based on Plasmonic Nanoparticles

Biosensors Based on Electropolymerized Films

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