Preparation and characterization of a lamotrigine imprinted polymer and its application for drug assay in human serum

Abstract

A molecularly imprinted polymer (MIP) against lamotrigine (LTG) was prepared, characterized, and its recognition properties were compared with a blank nonimprinted polymer (NIP). Two classes of binding sites were found in the MIP--high affinity (K(D) = 16.2 microM) and low affinity (K(D) = 161.3 microM). Selectivity of the synthesized MIP was examined using compounds with similar structures or therapeutic uses to LTG. In compounds which had structural similarity to LTG, the presence of amine groups appeared to affect binding to the MIP, however overall shape of the molecule was also important. Under the optimal conditions developed, other anticonvulsant drugs tested did not bind the MIP. A molecularly imprinted SPE (MISPE) procedure was developed which had a recovery of 84-89%, interday variation of less than 3.4% and intraday variation of less than 2.8%. The MISPE procedure was compared with a routine liquid-liquid extraction (LLE) procedure used for the HPLC determination of LTG in serum from patients. The data indicated that the MIP synthesized showed both good selectivity and high affinity for LTG and could be used for the extraction of the drug from serum samples or as the receptor layer for an LTG selective biosensor.

Application of molecularly imprinted hydrogel for the preparation of lactose‐free milk

“…The preparation of specific binding sites via the molecular imprinting technique has become the main objective of many studies and major interest of some researchers in recent years . Molecularly imprinted polymers (MIPs) are synthesised by radical polymerisation of monomers in the presence of a suitable template molecule.…”

“…The functional monomers are arranged around a template compound and are then fixed in a spatial arrangement with a cross‐linker monomer . The MIPs could be applied as the sorbent in solid‐phase extraction,, as the stationary phase in high‐performance liquid chromatography,, in biosensors, and as new drug delivery systems . Covalent and non‐covalent methods are two main strategies for preparation of MIPs .…”

Synthesis of Surface Molecularly Imprinting Polymers for Methylphenidate and its Application in Separating Methylphenidate

“…The binding sites of MIPs prepared by non-covalent bulk polymerization are usually heterogeneous. The KD value in other studies ranged from μM to M (lamotrigine KD=16.2 mM [23], sulphametoxazole KD=18.8 mM [24], theophylline KD=1.5 M [25].…”

“…3 mL acetic acid could be used to wash the polymer column after loading, without removing considerable amount of MPH from MIP-A1 cartridge. Acidic solutions are usually used for complete washing of template in elution step from the MIP column [23]. In this study, 8 mL of methanol-TFA (50:50, v/v) was used as eluting solvent.…”

Potentiometric Sensing of Lamotrigine Based on Molecularly Imprinted Polymers

“…The best conditions for extraction of LTG from the MIP was found to be washing for 14 hours with THF, using a soxhlet extractor. The MIP was finally washed with THF/TFA 90 % (50-50 v/v) and then conditioned with water [43].…”