Preparation and characterization of a thrombin inhibitor grafted polyethersulfone blending membrane with improved antithrombotic property

Fu, Shuangshuang; Ning, Jianping; Liao, Xiali; Fu, Xiao; Yang, Zheng-Bo

doi:10.1039/c5ra16515f

Cited by 22 publications

(11 citation statements)

References 46 publications

(43 reference statements)

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“…These membranes showed low hemolysis (0.40 ± 0.07%), prolonged blood coagulation time (activated partial thromboplastin time = 78.5 ± 1.0 s, prothrombin time = 19.1 ± 0.1 s, thrombin time = 25.2 ± 0.1 s, and fibrinogen time = 22.2 ± 1.0 s), and low level of activated terminal complement complex (5.9 ± 1.5 ng/mL). These values of hemolysis, blood coagulation times, and complement activation are better than the literature-reported values, [7][8][9][10][11]15,18,19 which indicates that the cGTP HFMs are suitable for the blood-contact applications, e.g., BAK application. The improved compatibility of cGTP HFMs with the human blood can be attributed to the presence of the biocompatible entities (GO, TPGS, and ECMs) in the surface-functionalized (cGTP) HFMs as discussed in the literature reports.…”

Section: Resultsmentioning

confidence: 82%

Surface-Functionalized Poly(Ether Sulfone) Composite Hollow Fiber Membranes with Improved Biocompatibility and Uremic Toxins Clearance for Bioartificial Kidney Application

Modi

Verma

Bellare

2020

ACS Appl. Bio Mater.

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Bioartificial kidney (BAK) is attracting the focus of the research community. In this study, the efficacy of surface-functionalized poly(ether sulfone)-TPGS-graphene oxide composite hollow fiber membranes as a promising material for the single extracorporeal unit for BAK application was evaluated. The cytotoxicity was examined using human primary renal proximal tubular epithelial cells (hPTCs), and the removal of uremic toxins (urea, creatinine, phosphate, and lysozyme) from the toxin-spiked goat blood was measured. The surface-functionalized polymer composite membranes acted as a biocompatible material for attachment and proliferation of hPTCs, which was confirmed by microscopy studies, proliferation, and activity assays. The functional activity of these renal cells over this biocompatible membrane was also maintained. Remarkably, the functionalized composite membranes showed removal of urea (46.4 ± 3.5%), creatinine (52.2 ± 3.9%), phosphate (35.5 ± 2.7%), and lysozyme (11.2 ± 0.8%) from the toxin-spiked goat blood. Therefore, these obtained results showed that the surface-functionalized poly(ether sulfone)-TPGS-graphene oxide composite hollow fiber membranes are suitable for BAK application.

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Section: Resultsmentioning

confidence: 82%

Surface-Functionalized Poly(Ether Sulfone) Composite Hollow Fiber Membranes with Improved Biocompatibility and Uremic Toxins Clearance for Bioartificial Kidney Application

Modi

Verma

Bellare

2020

ACS Appl. Bio Mater.

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“…As shown in Figure , APTT, PT, TT, and FT is the highest for PT‐NZ HFMs, among all the HFMs including the commercial F60S HFMs considered for the study. As seen from the Table , the clotting time (TT, APTT, PT, and FT) of PT‐NZ HFMs is higher than that of the majority membranes reported in literature except PT value of citric acid‐grafted‐polyurethane‐blended polyethersulfone (PES) membranes and APTT value of functionalized MWCNT‐based sulphonated‐PES membranes . The incorporation of TPGS results in prolonged time values for PT and PT‐NZ HFMs.…”

Section: Resultsmentioning

confidence: 87%

Improved hemodialysis with hemocompatible polyethersulfone hollow fiber membranes: In vitro performance

et al. 2017

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We show that addition of nanozeolite (NZ) and vitamin E D-α-Tocopherol polyethylene glycol succinate (TPGS or T) considerably improves the performance of polyethersulfone (PES or P) hollow fiber membrane (HFM) for hemodialysis. Nanocomposite HFMs were manufactured using PES as a polymer, TPGS as an additive and NZ as a filler to give a composite membrane called PT-NZ. HFMs were spun by dry-wet spinning principle based on liquid-liquid phase separation. TPGS and NZ were successfully incorporated in HFMs, as confirmed by EDX elemental mapping. The resultant PT-NZ HFMs had improved hemocompatibility: lower percent hemolysis (0.28% in batch mode and 0.32% in continuous mode), lower platelet adhesion, higher coagulation time and lower protein adsorption (16.34 µg/cm ), compared with P, PT, and commercial (F60S) HFMs. The ultrafiltration coefficient of PT-NZ HFM-based module (274.59 mL/m /h/mmHg) was ∼1.5-times higher than that of F60S membranes (151.67 mL/m /h/mmHg), and the solute rejection of both the membranes was comparable. The toxin clearance performance of lab-scale PT-NZ HFM-based hemodialyzer with uremic toxin spiked goat blood was remarkably higher (five times) than that of F60S. Hence, the synthesized PT-NZ HFMs are a potentially attractive membrane material for hemodialysis application, particularly due to decreased treatment time and minimal side reactions. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1286-1298, 2018.

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“…PES‐COOH was prepared by two‐step reactions: acetylation followed by oxidation . Initially, 7‐g PES were dried using an oven set with a temperature of 110°C for 1 hour.…”

Section: Methodsmentioning

confidence: 99%

“…In this work, the ratio of PES membrane (M‐1) was 20% w /w in respect to the solvent (NMP). On the basis of previous studies, it was found that the best blending ratio to the PES is 1: l; therefore, PES‐ABS and PES‐AMBS were blended to PES in a ratio of 1:1 as shown in Table (M‐2 and M‐6), respectively. To enhance the antibacterial properties, ZnO nanoparticles were added to the membrane in a ratio of 2%, 3%, and 5% w /w in which the nanoparticles were suspended first in NMP and sonicated for 30 minutes at 60°C in a water bath in order to suspend the ZnO nanoparticles.…”

Section: Methodsmentioning

confidence: 99%

Enhanced antifouling and anticoagulant properties of grafted biomolecule polyethersulfone membranes

Alenazi

Alamry

Hussein

et al. 2019

Polymers for Advanced Techs

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Polyethersulfone (PES) has been widely used in membrane technology and used to purify water in water treatments application or as a dialyzer to purify blood in hemodialysis. In this work, PES was chemically modified by separately grafting two biomolecules, 4‐aminobenzenesulfonamide (ABS), and 4‐amino‐N‐(5‐methylisoxazol‐3‐yl)benzenesulfonamide (AMBS), on PES backbone, and these modified membranes were blended to unmodified PES, in 1:1 ratio, in order to obtain PES‐b‐PES‐ABS and PES‐b‐PES‐AMBS membranes. The first aim of this study is to measure the anticoagulant properties of the modified membrane by measuring the activated partial thromboplastin time (APTT) and prothrombin time (PT). The second aim of the study is to evaluate the antifouling properties of the modified PES membranes by examining its antimicrobial activity against two Gram‐negative bacteria, which are Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli); two Gram‐positive bacteria, which are Bacillus subtilis (B. subtilis) and Staphylococcus aureus (S. aureus); and a fungus, which is Candida albicans (C. albicans). The results showed that grafting of ABS and AMBS improved overall the hydrophilicity properties of the modified PES membranes. PES‐b‐PES‐ABS membranes showed better anticoagulant properties with 13 seconds for PT and 38 seconds for APPT, in comparison with the control sample (pure plasma), which showed 12 seconds for PT and 30 seconds for APPT. For antimicrobial tests, both PES‐b‐PES‐ABS and PES‐b‐PES‐AMBS membranes did not show any antibacterial activity, but when zinc oxide (ZnO) nanoparticles were added to the modified PES membranes in concentrations between 3% to 5% w/w, PES‐b‐PES‐ABS‐ZnO (M‐4 and M‐5), and PES‐b‐PES‐AMBS‐ZnO (M‐8 and M‐9) nanocomposite membranes showed antibacterial activity against P. aeruginosa and S. aureus.

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Preparation and characterization of a thrombin inhibitor grafted polyethersulfone blending membrane with improved antithrombotic property

Abstract: A thrombin inhibitor grafted polyethersulfone membrane with improved antithrombotic property.

Cited by 22 publications

References 46 publications

Surface-Functionalized Poly(Ether Sulfone) Composite Hollow Fiber Membranes with Improved Biocompatibility and Uremic Toxins Clearance for Bioartificial Kidney Application

Surface-Functionalized Poly(Ether Sulfone) Composite Hollow Fiber Membranes with Improved Biocompatibility and Uremic Toxins Clearance for Bioartificial Kidney Application

Improved hemodialysis with hemocompatible polyethersulfone hollow fiber membranes: In vitro performance

Enhanced antifouling and anticoagulant properties of grafted biomolecule polyethersulfone membranes

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