Preparation and characterisation of antibacterial silver-containing nanofibres for wound healing in diabetic mice

Abstract

Impaired wound healing is a common complication of diabetes mellitus. Nanofibre can be served as powerful tools in advanced wound care management. The aim of this work is to evaluate the role of antibacterial nanofibres by incorporating silver nanoparticles into celluose acetate nanofibres as wound dressing on excisional wound healing in diabetic mice. Celluose acetate nanofibres were prepared by electrospinning technique. The prepared nanofibres were characterised using scanning electron microscope (SEM), and Fourier-transform infrared (FTIR) spectrophotometer. The antimicrobial activity was tested against Gram-positive and Gram-negative bacteria. A full thickness of the excision wound of circular area 95 mm 2 and 2 mm depth was created. Wound dressed by silver containing nanofibres, the wound closure rate were assessed, skin wounds were processed for histopathological examination Preparation and characterisation of antibacterial 83and comparing the mechanical properties of healed skin. It was found that, wounds dressed with silver containing nanofibres showed marked increase in collagen production which improve the skin mechanical properties.

Evaluation of keratin biomaterial containing silver nanoparticles as a potential wound dressing in full‐thickness skin wound model in diabetic mice

“…We also assessed the deposition and arrangement of collagen fibers during wound healing evidencing that FKDP-AgNP-treated wounds showed a marked increase in formation of collagen fibers and epithelial cells covering abilities. Similar results were obtained by Shalaby, Fekry, Sodfy, Sheredy, and Moustafa (2015), who examined silver-containing nanofibers as a wound dressing in an excisional full-thickness wound healing model in diabetic mice. They reported that wounds treated with silver nanofibers showed marked increase in collagen production improving skin mechanical properties.…”

Polymer-Based Wound Dressing Materials Loaded with Bioactive Agents: Potential Materials for the Treatment of Diabetic Wounds

“…The in vitro antibacterial studies displayed that the antibacterial effects of nanofibers increased against E. coli and S. aureus as the content of Ag nanoparticles increase. The in vivo studies demonstrated that diabetic lesions in mice treated with nanoparticle-loaded nanofiber exhibited significantly accelerated wound reduction compared to those treated with insulin [68]. Ag nanoparticles improved the antimicrobial effects of the nanofibers, making the polymeric nanofibers display rapid wound contraction.…”

Recent Advances in Electrospun Nanofiber-Based Strategies for Diabetic Wound Healing Application

“…Therefore, loading with bioactive agents on electrospun nanofiber dressings or dissolving the bioactive agents and polymers in the same solvent [ 115 ] can accelerate the healing process in chronic diseases, as shown in Figure 4 A [ 116 ]. Electrospun nanofibers could also deliver bioactive ingredients, antibiotics [ 12 ], antibacterial particles [ 117 ], stem cells [ 118 ], metal nanoparticles [ 119 ], and plant metabolites [ 120 , 121 ], which can activate cell proliferation, migration, and differentiation of damaged cell tissues. Hence, besides selecting different materials for rebuilding the natural scaffolds, electrospun nanofibers are also utilized as the delivery system for loading different bioactive agents to wound sites.…”