Preparation and application of a molecularly imprinted polymer for determination of glibenclamide residues

Abstract

The performance of a molecularly imprinted polymer (MIP) as selective packing material for solid-phase extraction (SPE) of residual glibenclamide in an industrial process was investigated. MIP was prepared using 4-vinylpyridine as monomer, ethylenedimethacrylate as cross linker, 2,2’-azobis-2-methylpropionitrile as initiator and dimethyl formamide as porogen. Use of acetonitrile as a rebinding solvent allows good recognition of the glibenclamide template. It was found that this polymer can be used for determination of trace levels of glibenclamide with a recovery percentage that could reach 87.1 %. Furthermore, the synthesized MIP showed higher selectivity towards glibenclamide than other compounds such as glimepiride and metformine. The synthesized MIP enabled direct determination of the target contaminant after an enrichment step that allowed quantification of glibenclamide at a concentration as low as 0.016 mg L-1. Combination of high performance liquid chromatography with MIP-SPE could be successfully used for quality control of pharmaceuticals during the cleaning process in the production of dry drug forms.

Ultrasensitive detection of glibenclamide based on its enhancing effect on the fluorescence emission of CdTe quantum dots

“…Glibenclamide (GB) or glyburide (5‐chloro‐ N ‐[2‐[4[(cyclohexylamino)carbonyl]amino]sulfonyl]phenyl]ethyl]‐2‐methoxy benzamide) (Figure ) is known as a common drug for the treatment type 2 diabetes . It can be quickly absorbed from the digestive system and act as a powerful and long‐term antidiabetic agent . GB is related to the second generation of sulfonylureas and can perform the antidiabetic role via increasing the release of internal insulin from pancreas beta cells.…”

“…The literature survey showed that the detection of GB in pharmaceuticals and biological liquids has been commonly based on the high‐performance liquid chromatography (HPLC) techniques with various detection systems, such as UV, fluorescence or mass spectrometry . These methods are tedious, time‐consuming, high‐priced, difficult and need a skilled operator .…”

Development of Nanozyme-Labeled Biomimetic Immunoassay for Determination of Sulfadiazine Residue in Foods

“…To overcome this problem, many studies have focused on developing bionic antibodies. Molecularly imprinted polymer (MIP) has many complementary cavities to the template molecule in shape, size, and chemical function, making them specific and selective to the target molecule [15][16][17]. During the past decades, the increasing trend of imprinting biologically active compounds ranging from small pharmaceutical molecules to large biomacromolecules including myoglobin, naproxen, antioxidants, diclofenac and urea has been numerously reported [18][19][20][21][22].…”

A novel molecular imprinting polymer for the selective adsorptionof D-arabinitol from spiked urine

“…Besides, the noncovalent approach is experimentally more straight forward relative to the covalent printing method because the stage of its complexity is achieved only by mixing the template with functional monomers in the appropriate solvent. Such did not require template derivatization, and template removal is usually sufficient by washing polymers [21]. One of the most reliable modern analytical methods is liquid chromatography tandem-mass spectrometry (LC-MS), which has advantages of both accurate identification and quantification of analyte target.…”