Prenatal sevoflurane exposure causes neuronal excitatory/inhibitory imbalance in the prefrontal cortex and neurofunctional abnormality in rats

Zhao, Tianyun; Chen, Yanxin; Sun, Zhixiang; Shi, Ziwen; Qin, Jingwen; Lu, Junming; Li, Chuanxiang; Ma, Ding; Zhou, Libing; Song, Xingrong

doi:10.1016/j.nbd.2020.105121

Cited by 22 publications

(16 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, CRS promoted the occurrence of breast cancer in MMTV-PyMT mice, which was also disrupted by the antidepressant drug. dopamine, adrenaline, and noradrenaline (31,32). Recent studies have shown that damage to the normal structure and function of microglia is due to strong inflammatory activation or decline, and chronic unpredictable stress exposure may lead to depression and related disorders in neuroplasticity and neurogenesis.…”

Section: Discussionmentioning

confidence: 99%

The central nervous system can directly regulate breast cancer progression and blockage by quercetin

Luo¹,

Zhang²,

Liu³

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

Background: Neuroinflammation involving the central nervous system (CNS), such as depression, is associated with a significantly increased risk of cancer and cancer-specific mortality due to breast cancer. It is of great significance to learn about the regulatory process of CNS in breast cancer progression.Methods: We established a depressive MMTV-PyVT mouse model. The expression levels of neurotransmitters in the serum of depression animal models were assessed by enzyme-linked immunosorbent assay (ELISA). Changes of the microglia cells in the mice's brains were evaluated by immunofluorescence and reverse transcription-polymerase chain reaction (RT-PCR). Breast cancer progression was assessed by immunohistochemistry (IHC) analysis. To further investigate the mechanism by which ant-depressant drugs disrupt breast cancer progression, protein sequencing and network pharmacology were applied to identify related targets. Furthermore, we used conditioned medium from BV-2 microglia to culture breast cancer cells and treated the cells with quercetin at different concentrations; cell viability was assessed by the MTT assay.Results: Our results show a possible regulatory target between neuroinflammation in the CNS and development of breast cancer, along with the reversal effect of quercetin on breast cancer progression.Conclusions: Chronic stress may be an indicator of breast cancer and that quercetin could be an effective treatment for breast cancer patients with chronic stress.

show abstract

Section: Discussionmentioning

confidence: 99%

The central nervous system can directly regulate breast cancer progression and blockage by quercetin

Luo¹,

Zhang²,

Liu³

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

show abstract

“…First, measurement error. Second, the repeated anesthesia during our experiment may influence brain volume in our macaques [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Longitudinal trajectories of brain volume in combined antiretroviral therapy treated and untreated simian immunodeficiency virus-infected rhesus macaques

Liú

Liu

et al. 2021

Aids

View full text Add to dashboard Cite

Objectives: We used simian immunodeficiency virus (SIV)-infected nonhuman primates to investigate longitudinal changes of brain volume caused by SIV and the effect of combined antiretroviral therapy (cART). In addition, the relation between viral load, immune status, and brain volume were explored. Design: A longitudinal study of two healthy controls, five SIV mac239 -infected macaques received cART (SIV+cART+) at 40 days postinnoculation, and five SIV mac239 -infected macaques received no therapy (SIV+cART−). Methods: Structural T1-weighted MRI, blood and cerebrospinal fluid testing were acquired at multiple time points for 48 weeks postinfection (wpi). Brain volume was estimated using region of interest (ROI)-based analysis. Volume differences were compared among three groups. Linear regression models tested the associations between brain volumes and biomarkers (viral load, CD4 + T-cell count, CD4 + /CD8 + ratio). Results: In our model, brain volume alteration in SIV-infected macaques can be detected at 12 wpi in several brain regions. As the infection progresses, the SIV+cART− macaques displayed generalized gray matter atrophy at the endpoint. Though initiate cART right after acute infection, SIV+cART+ macaques still displayed brain atrophy but showed signs of reversibility. Plasma viral load is mainly associated with subcortical nucleus volume whereas CD4 + T-cell count and CD4 + /CD8 + ratio in plasma were associated with widespread cortical volume. Conclusion: The SIV mac239 -infected Chinese origin macaque is a valid model for neuroHIV. Brain atrophy caused by SIV infection can be relieved, even reversed, by cART. Our model also provides new insights into understanding the pathogenesis of brain injury in people with HIV (PWH).

show abstract

“…Sevoflurane, a volatile anesthetic agent, can disrupt consciousness and cognition. It has also been shown to lead to synaptic and myelin damage, neuroinflammation, excitatory neuron abnormality, and neuronal apoptosis in the brains of young animals (Xie et al, 2020;Zhao et al, 2020). To explore the molecular mechanism underlying anesthesia and sevoflurane-induced deficiencies, a number of transcriptome studies using RNA sequencing (RNA-seq) techniques have been conducted.…”

Section: Introductionmentioning

confidence: 99%

“…Various brain regions were included in such studies, especially the striatum, hypothalamus, prefrontal cortex (PFC), and hippocampus (Song et al, 2019;Zhang et al, 2019a;Xie et al, 2020;Yamamoto et al, 2020;Zhao et al, 2020;Zhang L. et al, 2021). The differentially expressed genes (DEGs) identified by transcriptome studies and verified by subsequent experiments have helped to explain the observed adverse effects of sevoflurane.…”

Section: Introductionmentioning

confidence: 99%

Identification of Prefrontal Cortex and Amygdala Expressed Genes Associated With Sevoflurane Anesthesia on Non-human Primate

Cheng

Liu

Zhang

et al. 2022

Front. Integr. Neurosci.

View full text Add to dashboard Cite

Clinical trials and animal studies have indicated that long-term use or multiple administrations of anesthesia may lead to fine motor impairment in the developing brain. Most studies on anesthesia-induced neurotoxicity have focused on the hippocampus and prefrontal cortex (PFC); however, the role of other vital encephalic regions, such as the amygdala, is still unclear. Herein, we focused on sevoflurane, the most commonly used volatile anesthetic in infants, and performed a transcriptional analysis of the PFC and amygdala of macaques after multiple exposures to the anesthetic by RNA sequencing. The overall, overlapping, and encephalic region-specific transcriptional patterns were separately analyzed to reveal their functions and differentially expressed gene sets that were influenced by sevoflurane. Specifically, functional, protein–protein interaction, neighbor gene network, and gene set enrichment analyses were performed. Further, we built the basic molecular feature of the amygdala by comparing it to the PFC. In comparison with the amygdala’s changing pattern following sevoflurane exposure, functional annotations of the PFC were more enriched in glial cell-related biological functions than in neuron and synapsis development. Taken together, transcriptional studies and bioinformatics analyses allow for an improved understanding of the primate PFC and amygdala.

show abstract

Prenatal sevoflurane exposure causes neuronal excitatory/inhibitory imbalance in the prefrontal cortex and neurofunctional abnormality in rats

Cited by 22 publications

References 51 publications

The central nervous system can directly regulate breast cancer progression and blockage by quercetin

The central nervous system can directly regulate breast cancer progression and blockage by quercetin

Longitudinal trajectories of brain volume in combined antiretroviral therapy treated and untreated simian immunodeficiency virus-infected rhesus macaques

Identification of Prefrontal Cortex and Amygdala Expressed Genes Associated With Sevoflurane Anesthesia on Non-human Primate

Contact Info

Product

Resources

About