Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-enk expression in adolescent rats

Abate, Paula; Reyes-Guzmán, A.C.; Hernández-Fonseca, Karla; Méndez, Milagros

doi:10.1016/j.npep.2016.11.006

Cited by 11 publications

(7 citation statements)

References 97 publications

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“…Our new finding, that the low concentration of 0.1% EtOH produces a long lasting increase in freezing behavior only under novel conditions and not after habituation to the environment, suggests that only the immediate response to the novel environment is affected by embryonic EtOH exposure and that these novelty-induced behaviors may reflect an elevation in anxiety or fear 59 . Although studies of locomotor activity 35 , 36 typically fail to distinguish between behavioral responses to novelty and behaviors after habituation and generally average behavior over the entire testing session, our results here in both larvae and adult fish are consistent with a study in adolescent rats showing prenatal EtOH exposure to reduce horizontal locomotion and rearing behavior in response to novelty but not after habituation 60 . Our additional findings in the novel tank test, showing that EtOH consistent with prior reports 11 , 61 reduces anxiety-like behaviors under novel conditions only at the 0.5% but not 0.1% concentration while increasing Hcrt neurons only at the 0.1% concentration, suggests that the reduced anxiety at the 0.5% dose in adult females may be attributed to a mechanism other than Hcrt, such as a reduced release of cortisol that is also observed at higher EtOH concentrations in zebrafish 62 .…”

Section: Discussionsupporting

confidence: 88%

Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish

Collier

Yasmin

Khalizova

et al. 2021

Sci Rep

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Neurons expressing the neuropeptide hypocretin/orexin (Hcrt) in the hypothalamus promote reward-related behaviors including alcohol consumption and are shown in rodents and zebrafish to be stimulated by embryonic exposure to ethanol (EtOH). We used here in zebrafish three-dimensional analyses of the entire population of Hcrt neurons to examine how embryonic EtOH exposure at low-moderate concentrations (0.1% or 0.5% v/v) alters these neurons in relation to behavior. We found that EtOH in the water for 2 h (22–24 h post fertilization) increases the number of Hcrt neurons on the left but not right side of the brain through a stimulation of cell proliferation, this is accompanied by a decrease in locomotor activity under novel conditions but not after habituation, and these effects are evident in both larvae and adults indicating they are long lasting. Our analyses in adults revealed sexually dimorphic effects, with females consuming more EtOH-gelatin and exhibiting more freezing behavior along with an asymmetric increase in Hcrt neurons and males exhibiting increased aggression with no change in Hcrt. These findings suggest that a long lasting, asymmetric increase in Hcrt neurons induced by EtOH results from an asymmetric increase in proliferation specific to Hcrt and contributes to behavioral changes in females.

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Section: Discussionsupporting

confidence: 88%

Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish

Collier

Yasmin

Khalizova

et al. 2021

Sci Rep

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“…Similarly, previous animal studies reported increases in anxiety-like behaviour following prenatal alcohol exposure (Cullen et al, 2013;Dursun et al, 2006;Kleiber et al, 2011;Liang et al, 2014). Moreover, alterations in locomotor activity have been described in rodent offspring prenatally exposed to alcohol (Abate et al, 2017;Dursun et al, 2006;Kim et al, 2013;Muñoz-Villegas et al, 2017;Sanchez Vega et al, 2013;Schambra et al, 2016).…”

Section: Introductionsupporting

confidence: 57%

Altered brain functional connectivity and behaviour in a mouse model of maternal alcohol binge-drinking

Cantacorps

González-Pardo

Árias

et al. 2018

Progress in Neuro-Psychopharmacology and Biological Psychiatry

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Prenatal and perinatal alcohol exposure caused by maternal alcohol intake during gestation and lactation periods can have long-lasting detrimental effects on the brain development and behaviour of offspring. Children diagnosed with Foetal Alcohol Spectrum Disorders (FASD) display a wide range of cognitive, emotional and motor deficits, together with characteristic morphological abnormalities. Maternal alcohol binge drinking is particularly harmful for foetal and early postnatal brain development, as it involves exposure to high levels of alcohol over short periods of time. However, little is known about the long-term effects of maternal alcohol binge drinking on brain function and behaviour. To address this issue, we used pregnant C57BL/6 female mice with time-limited access to a 20% v/v alcohol solution as a procedure to model alcohol binge drinking during gestation and lactational periods. Male offspring were behaviourally tested during adolescence (30 days) and adulthood (60 days), and baseline neural metabolic capacity of brain regions sensitive to alcohol effects were also evaluated in adult animals from both groups. Our results show that prenatal and postnatal alcohol exposure caused age-dependent changes in spontaneous locomotor activity, increased anxiety-like behaviour and attenuated alcohol-induced conditioned place preference in adults. Also, significant changes in neural metabolic capacity using cytochrome c oxidase (CCO) quantitative histochemistry were found in the hippocampal dentate gyrus, the mammillary bodies, the ventral tegmental area, the lateral habenula and the central lobules of the cerebellum in adult mice with prenatal and postnatal alcohol exposure. In addition, the analysis of interregional CCO activity correlations in alcohol-exposed adult mice showed disrupted functional brain connectivity involving the limbic, brainstem, and cerebellar regions. Finally, increased neurogenesis was found in the dentate gyrus of the hippocampus of alcohol-exposed offspring, suggesting neuroadaptive effects due to early alcohol exposure. Our results demonstrate that maternal binge-like alcohol drinking causes long-lasting effects on motor and emotional-related behaviours associated with impaired neuronal metabolic capacity and altered functional brain connectivity.

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“…MET-ENK levels are specifically changed by fetal intoxication in the abovementioned central areas during adolescence. These neural enkephalinergic changes modulate later adolescent sensitivity to different motor effects of EtOH (Abate et al, 2017). Interestingly, fetal alcohol experiences also increased levels of mu-opioid receptor transcripts after intake without affecting DOR or KOR receptor transcripts in adolescents (Fabio et al, 2015).…”

Section: Role Of the Opioid System In The Modulation Of Prenatal Etohmentioning

confidence: 91%

Fetal Alcohol Programming of Subsequent Alcohol Affinity: A Review Based on Preclinical, Clinical and Epidemiological Studies

Miranda-Morales

D'aloisio

Anunziata

et al. 2020

Front. Behav. Neurosci.

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Prenatal ethanol exposure modifies locomotor activity and induces selective changes in Met-enk expression in adolescent rats

Cited by 11 publications

References 97 publications

Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish

Sexually dimorphic and asymmetric effects of embryonic ethanol exposure on hypocretin/orexin neurons as related to behavioral changes in zebrafish

Altered brain functional connectivity and behaviour in a mouse model of maternal alcohol binge-drinking

Fetal Alcohol Programming of Subsequent Alcohol Affinity: A Review Based on Preclinical, Clinical and Epidemiological Studies

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