We present our initial experience of using HDlive Flow with the HDlive silhouette mode for diagnosing fetal diaphragmatic eventration in one of a pair of monochorionic diamniotic twin fetuses at 21 weeks and 4 days of gestation. Two-dimensional (2D) sonography suggested abnormal elevation of the right hemidiaphragm. Power Doppler ultrasound revealed upward-deviated right pulmonary and hepatic vessels, which were demarcated by the right hemidiaphragm. HDlive Flow with the HDlive silhouette mode clearly depicted the upward-deviated right pulmonary and hepatic vessels, which were separated by the high position of the right hemidiaphragm. The spatial relationships among the fetal liver, diaphragm, lung, and heart could be clearly recognized using HDlive Flow with the HDlive silhouette mode. MRI also strongly suggested right diaphragmatic eventration at 30 weeks and 4 days of gestation. HDlive Flow with the HDlive silhouette mode might be beneficial as an additional diagnostic tool along with conventional 2D sonography, and it facilitates early differentiation between fetal diaphragmatic eventration and diaphragmatic hernia.