Prenatal administration of dexamethasone during early pregnancy negatively affects placental development and function in mice1

Lee, Ji Yeon; Park, S. J.; Kim, S. H.; Kim, M. H.

doi:10.2527/jas.2012-5090

Cited by 12 publications

(19 citation statements)

References 35 publications

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“…We have recently demonstrated that administration of dexamethasone (DEX), a synthetic GC, during the gestational day 7.5~9.5 of pregnant mice causes placental defect and embryonic growth restriction in a sex-dependent manner (Lee et al, 2012;Yun et al, 2014). Here, we examined the effect of prenatal DEX treatment on fetal skeletal development on day 18.5.…”

Section: A Few Studies Have Demonstrated the Effect Of Prenatalmentioning

confidence: 99%

“…Pregnant ICR mice were ordered from Orient Biology (Sungnam, Korea). DEX administration was performed as previously described (Lee et al, 2012). Briefly, either saline (control group) or dexamethasone 21-phosphate disodium salt (1 mg/ kg or 10 mg/kg, Sigma-Aldrich, St. Louis, MO, USA) was injected on days 7.5, 8.5 and 9.5 of gestation intraperitoneally.…”

Section: Experimental Animals and Injection Of Dexmentioning

confidence: 99%

“…Although it is not clear how embryos strive for survival, there should be some complementary mechanisms to overcome unfavorable intrauterine conditions, which could be one reason for less effect with high dose of DEX. We have already demonstrated that a subgroup of placentas from DEX10-injected mother was enlarged with structural abnormalities (Lee et al, 2012). Placentomegaly has been also observed in maternally stressed animal models and human, and therefore, it was regarded as a complementary survival strategy to adapt to an adverse condition (Faichney and White 1987;Lumey 1998;McCrabb et al, 1992;Tegethoff et al, 2010).…”

Section: Effect Of Prenatal Dex Treatment On the Protrusion Of Xiphoimentioning

confidence: 99%

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Prenatal Stress Induces Skeletal Malformations in Mouse Embryos

Kim

Yun

Lee

et al. 2015

BSL

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Dexamethasone, a synthetic glucocorticoid (GC), is clinically administered to woman at risk for premature labor to induce fetal lung maturation. However, exposure to repeated or excess GCs leads to intrauterine growth restriction (IUGR) and subsequently increases risk of psychiatric and cardio-metabolic diseases in later life through fetal programming mechanisms. GCs are key mediators of stress responses, therefore, maternal nutrient restriction or psychological stress during pregnancy also causes negative impacts on birth and neurodevelopment outcome of fetuses, and other congenital defects, such as craniofacial and skeletal abnormalities. In this study, to examine the effect of prenatal stress on fetal skeletal development, dexamethasone (1 mg/kg [DEX1] or 10 mg/kg [DEX10] maternal body weight per day) was administered intraperitoneally at gestational day 7.5~9.5 and the skeletons were prepared from embryos at day 18.5. Seven out of eighteen (39%) embryos treated with DEX10 showed axial skeletal abnormalities in either the T13 or L1 vertebrae. In addition, examination of the sternum revealed that xiphoid process, the protrusive triangular part of the lower end of the sternum, was bent more outward or inward in DEX group embryos. In conclusion, our findings suggest a possible link to the understanding of the effect of uterine environment to the fetal skeletal features.

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Section: A Few Studies Have Demonstrated the Effect Of Prenatalmentioning

confidence: 99%

Section: Experimental Animals and Injection Of Dexmentioning

confidence: 99%

Section: Effect Of Prenatal Dex Treatment On the Protrusion Of Xiphoimentioning

confidence: 99%

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Prenatal Stress Induces Skeletal Malformations in Mouse Embryos

Kim

Yun

Lee

et al. 2015

BSL

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“…Nevertheless, obstetri cians and gynecologists have not agreed upon the need for treatment or the methods of treatment for hyperandrogenism in pregnant women. 23,24 In connection with this disagreement, studies of the influence of excess maternal androgen on the development and function of the maternal-placental complex are unclear.…”

Section: Introductionmentioning

confidence: 99%

“…Some studies have shown decreases in fetal and placental weights because of excess maternal androgen. 24,36,38 …”

Section: Introductionmentioning

confidence: 99%

Ultrasound Features of the Formation of the Maternal-Placental Complex in Cases of Hyperandrogenism as Observed during the First and Second Trimesters of Pregnancy

Olga¹,

Elena²,

Luybov³

et al. 2014

Donald School Journal of Ultrasound in Obstetrics and Gynecology

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An excess of maternal androgen adversely affects the implan tation process and contributes to uterine circulatory disturbances and myometrium vessel sclerosis. Placental insufficiency is typi cally diagnosed at the end of the second trimester of pregnancy or at the beginning of the third trimester, when the treatment is not effective.Many researchers have attempted to use early sonographic diagnostics to identify placental failure in various pathological states. Nevertheless, the peculiarities of the formation of the fetal-placental complex during the first and second trimesters are still undefined. In this study, we traced the process of placental formation under conditions of excess maternal androgen using ultrasound and Dopplerometry. Morphological examinations of the сhorion and placental tissue were undertaken to сompare any changes.Unfavorable chorion locations, heterogeneous chorion tis sue structures, and marginal sinus dilatations were revealed by ultrasound. According to a Dopplerometric investigation, a statistically significant increase in peripheral vessel resistance in cases of hyperandrogenism was identified compared with healthy pregnancies. The negative influence of hyperandrogenism on placental formation was confirmed by morphologi cal data (including the presence of immature decidual cells, defec tive villi that covered the epithelium, and the dissociative development of cotyledons).

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Biological Effects of Exposure to a Radiofrequency Electromagnetic Field on the Placental Barrier in Pregnant Rats

Kim

Choi

Pack

et al. 2021

Bioelectromagnetics

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The placenta protects the fetus against excessive stress-associated maternal cortisol during pregnancy. We studied whether exposure to radiofrequency electromagnetic field (RF-EMF) radiation during pregnancy can cause changes in dams and their placentas. Pregnant Sprague-Dawley rats were divided into cagecontrol, sham-exposed, and RF-exposed groups. They were exposed to RF-EMF signals at a whole-body specific absorption rate of 4 W/kg for 8 h/day from gestational Day 1 to 19. Levels of cortisol in the blood, adrenal gland, and placenta were measured by enzyme-linked immunosorbent assay. Levels of adrenocorticotropic hormone and corticotropin-releasing hormone were monitored in maternal blood. Expression levels of placental 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) messenger RNA (mRNA) were measured by reverse transcription polymerase chain reaction. Morphological changes in the placenta were analyzed using hematoxylin and eosin staining. Fetal parts of the placenta were measured using Zen 2.3 blue edition software. Maternal cortisol in circulating blood (RF: 230 ± 24.6 ng/ml and Sham: 156 ± 8.3 ng/ml) and the adrenal gland (RF: 58.3 ± 4.5 ng/ml and Sham: 30 ± 3.8 ng/ml) was significantly increased in the RF-exposed group (P < 0.05). Placental cortisol was stably maintained, and the level of placental 11β-HSD2 mRNA expression was not changed in the RF-exposed group. RF-EMF exposure during pregnancy caused a significant elevation of cortisol levels in circulating blood; however, no changes in the placental barrier were observed in pregnant rats.

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Prenatal administration of dexamethasone during early pregnancy negatively affects placental development and function in mice1

Cited by 12 publications

References 35 publications

Prenatal Stress Induces Skeletal Malformations in Mouse Embryos

Prenatal Stress Induces Skeletal Malformations in Mouse Embryos

Ultrasound Features of the Formation of the Maternal-Placental Complex in Cases of Hyperandrogenism as Observed during the First and Second Trimesters of Pregnancy

Biological Effects of Exposure to a Radiofrequency Electromagnetic Field on the Placental Barrier in Pregnant Rats

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