Preliminary X-ray studies of the interaction of salmon sperm DNA with spermine

Huse, Yukiko; Mitsui, Yukio; Iitaka, Yōichi

doi:10.1016/0022-2836(78)90107-9

Cited by 16 publications

(5 citation statements)

References 15 publications

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“…There is little information on the structure of polyamine-DNA complexes. The X-ray fiber diffraction pattern of the complex of calf thymus and salmon sperm DNA with spermine, however, indicates that at 92% relative humidity the complex has the same helical parameters as B DNA (Suwalsky et al, 1969;Huse et al, 1978).…”

Section: Resultsmentioning

confidence: 93%

Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases

Pingoud¹,

Urbanke²,

Alves³

et al. 1984

Biochemistry

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We have investigated the effect of the polyamines spermine, spermidine, and putrescine and the prokaryotic histone-like proteins NS1 and NS2 on the restriction endonuclease EcoRI catalyzed cleavage of plasmid and bacteriophage DNAs. At low concentrations of spermine and spermidine, the rate of DNA cleavage by EcoRI is increased, while high concentrations of spermine as well as of spermidine are inhibitory. These phenomena are also observed with other restriction endonucleases. They are, therefore, probably due to the interaction of the polyamines with the DNA. Putrescine does not have such an effect within the concentration range investigated. Remarkably, low concentrations of spermine and spermidine very efficiently suppress EcoRI activity. An inhibition of the EcoRI-catalyzed cleavage of DNA is also observed with NS1 and NS2, an effect that can be mimicked with other basic proteins that interact with DNA. The results are discussed in terms of the mechanism of restriction in vivo.

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Section: Resultsmentioning

confidence: 93%

Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases

Pingoud¹,

Urbanke²,

Alves³

et al. 1984

Biochemistry

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“…In the dodecamer double helix formed by d(CGCGAATTCGCG) (Section 11.2), a spermine molecule straddles the major groove and is oriented nearly vertical to the helix axis, in contrast to previous suggestions (1165)(1166)(1167). Besides forming salt bridges between terminal amino groups and phosphates on both polynucleotide chains, one of the spermine's internal ammonium nitrogens is in direct hydrogen-bonding contact with guanine Os jutting out into the major groove.…”

Section: Experimental Evidence For Primary and Secondary Hydration Shmentioning

confidence: 98%

“…The B-DNA-type double helix can be stabilized in aqueous solution if DNA is complexed with nucleoprotamine or with homopolymer polypeptides like polylysine or poly arginine (1164). These basic polymers are probably arranged in the minor groove and, as discussed for the polyamines spermine and spermidine (Section 15.6), they cross-link phosphates of two oppositely directed polynucleotide chains (1165)(1166)(1167). In these two polyamines and in the related putrescine and cadaverine (Scheme 15-1), the N+ .…”

Section: Experimental Evidence For Primary and Secondary Hydration Shmentioning

confidence: 99%

Principles of Nucleic Acid Structure

Saenger

1984

Springer Advanced Texts in Chemistry

5,968

312

6,279

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“…Polyamines have also been shown to stabilize duplex DNA against thermal denaturation (Thomas & Bloomfield, 1984) and to convert synthetic polynucleotides from B-DNA to Z-DNA in a variety of cases (Behe & Felsenfeld, 1981;Chen et al, 1984;Rao et al, 1990). The interaction of polyamines with A-DNA has also been reported (Huse et al, 1978;Minyat et al, 1978;Jain et al, 1987).…”

mentioning

confidence: 99%

An A-form of poly(amino2dA-dT).cntdot.poly(amino2dA-dT) induced by polyamines

Garriga¹,

Quintana²,

Sági³

et al. 1993

Biochemistry

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The effect of the naturally occurring polyamines spermidine and spermine on poly(amino2-dA-dT).poly(amino2dA-dT) conformation has been studied by UV, CD, and IR spectroscopies. It is shown that a conformational transition is induced in poly(amino2dA-dT).poly(amino2dA-dT) by micromolar concentrations of the polyamines (30 microM) in low-salt aqueous solution. The analysis of our results, in view of previously published studies on conformational properties of the amino polynucleotide, indicates the resulting conformer to be an A-form. Interestingly, the polyamine concentration at the midpoint of the transition is the same in both cases. This provides further evidence that the coordination of positively charged counterions to DNA is determined largely from the DNA structure, probably with an important role for the sequence, and less from the nature of the ions.

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Preliminary X-ray studies of the interaction of salmon sperm DNA with spermine

Cited by 16 publications

References 15 publications

Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases

Effect of polyamines and basic proteins on cleavage of DNA by restriction endonucleases

Principles of Nucleic Acid Structure

An A-form of poly(amino2dA-dT).cntdot.poly(amino2dA-dT) induced by polyamines

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