An experiment was carried out in 2017 and 2018 planting seasons in Calabar, Southern Nigeria to evaluate the influence of nitrogen fertilizer on quality attributes of three clones of Sugarcane. The trial was laid out in 3 x 4 Split-plot in a randomized complete block design with 3 replications. The main plot was the three cane varieties (KN-10; OG-11 and OY-10), while the sup-plots consisted of the nitrogen fertilizer rates-0, 60, 90 and 120kgha-1N, (Symbolized N 1 , N 2 , N 3 and N 4 respectively). The results showed that none of the clones matured before the 12th month of growth, hence juice analysis took place from the 291 st day, for better brix, pol, purity and C.C.S quality attributes. Varietal differences as influence by treatment was glaring. The variety KN-10 at the application of 60kgha-1 N had brix 19.5%; pol 18.4%; purity 85.5%; cane yield 73.53 (tons/ha); average yield of sugar 9.0 (tons/ha), compared with 0kgha-1 N, and these values were significantly (P<0.05) higher than values obtained from either OG-11 or OY-10, given similar experimental conditions. The highest fibre content 18.4% was obtained from OY-10, where 120 kgha-1 N was applied. Most of the variations due to nitrogen was accounted for by a quadratic type, response curve. These results are discussed in relation to future fertilizer experiments envisaged for the potential sugarcane land in Southern Nigeria.