Preliminary study on the quick detection of acquired immure deficiency syndrome by saliva analysis using surface enhanced Raman spectroscopic technique

In clinical practice, one objective is to obtain diagnostic information while minimizing the invasiveness of the tests and the pain for the patients. To this end, tests based on the interaction of light with readily available biofluids including blood, urine, or saliva are highly desirable. In this review we examine the state of the art regarding the use of surface-enhanced Raman spectroscopy (SERS) to investigate biofluids, focusing on diagnostic applications. First, a critical evaluation of the experimental aspects involved in the collection of SERS spectra is presented; different substrate types are introduced, with a clear distinction between colloidal and non-colloidal metal nanostructures. Then the effect of the excitation wavelength is discussed, along with anomalous bands and artifacts which might affect SERS spectra of biofluids. The central part of the review examines the literature available on the SERS spectra of blood, plasma, serum, urine, saliva, tears, and semen. Finally, diagnostic applications are critically discussed in the context of the published evidence; this section clearly reveals that SERS of biofluids is most promising as a rapid, cheap, and non-invasive tool for mass screening for cancer.

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Methods in Raman spectroscopy for saliva studies – a review

Hardy

Kelleher

Gomes

et al. 2021

Applied Spectroscopy Reviews

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The use of Raman spectroscopy combined with saliva is an exciting emerging spectroscopy-biofluid combination. In this review, we summarize current methods employed in such studies, in particular the collection, pretreatment, and storage of saliva, as well as measurement procedures and Raman parameters used. Given the need for sensitive detection, surface-enhanced Raman methods are also surveyed, alongside chemometric techniques. A meta-analysis of variables is compiled. We observe a wide range of approaches and conclude that standardization of methods and progress to more extensive validation Raman-saliva studies is necessary. Nevertheless, the studies show tremendous promise toward the improvement of speed, diagnostic accuracy, and portable device possibilities in applications such as healthcare, law enforcement, and forensics.

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Preliminary study on the quick detection of acquired immure deficiency syndrome by saliva analysis using surface enhanced Raman spectroscopic technique

Cited by 8 publications

References 8 publications

PCA Kernel Based Extreme Learning Machine Model for Detection of NS1 from Salivary SERS Spectra

PCA Kernel Based Extreme Learning Machine Model for Detection of NS1 from Salivary SERS Spectra

Label-free surface-enhanced Raman spectroscopy of biofluids: fundamental aspects and diagnostic applications

Methods in Raman spectroscopy for saliva studies – a review

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