Surface Enhanced Raman spectroscopy (SERS) is an enhanced technique of Raman spectroscopy, which amplifies the intensity of Raman scattering to a practical range with adsorption of analyte onto nano-size plasmonic material such as gold, silver or copper. This feature of SERS has given it a niche in tracing molecular structure, especially useful for marking diseases specific biomarker. NS1 protein has been clinically accepted as an alternative biomarker for diseases caused by flavivirus. Detection of Nonstructural Protein 1 (NS1) will allow early diagnosis of the diseases. Its presence in the blood serum has been reported as early as first day of infection. With gold substrate, our work here intends to explore if SERS is suitable to detect NS1 from saliva, with saliva becoming the most favored alternative to blood as diagnostic fluid due to its advantages in sample collection. Our experimental results find both gold coated slide (GS) and saliva being Raman inactive, but the molecular fingerprint of NS1 protein at Raman shift 1012 cm(-1), which has never been reported before. The distinct peak is discovered to be attributed by breathing vibration of the benzene ring structure of NS1 side chain molecule. The characteristic peak is also found to vary in direct proportion to concentration of the NS1-saliva mixture, with a correlation coefficient of +0.96118 and a standard error estimation of 0.11382.
Dyslexia is referred as learning disability that causes learner having difficulties in decoding, reading and writing words. This disability associates with learning processing region in the human brain. Activities in this region can be examined using electroencephalogram (EEG) which record electrical activity during learning process. This study looks into performance of Support Vector Machine (SVM) using RBF kernel in classifying EEG signal of Normal, Poor and Capable Dyslexic children during writing words and non-words. Discrete Wavelet Transform (DWT) with Daubechies order 2 was employed to extract the power of beta and theta waves of EEG signal. Beta and Theta/Beta ratio form the input features for classifier. Multiclass one versus one SVM was used in the classification where RBF kernel parameters and box constraint values were varied with the factor of 10 to analyze performance of the classifier. It was found that the best performance of SVM with 91% overall accuracy was obtained when both kernel scale and box constraint are set to one.
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