Preliminary Study of Phenolic Glycosides from<i>Origanum majorana</i>, Quantitative Estimation of Arbutin; Cytotoxic Activity of Hydroquinone

Assaf, Mahmoud H.; Ali, Akbar; Makboul, Mohamed; Beck, Jean‐Paul; Anton, Roman

doi:10.1055/s-2006-962734

Cited by 45 publications

(18 citation statements)

References 5 publications

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“…It was reported that thymol and carvacrol represent the major constituents of the essential oils of Origanum species (Sarer et al, 1982). From Egyptian majorana (Origanum vulgare), arbutin and methyl arbutin have been already isolated and quantitatively estimated (Assaf et al, 1987). The present study deals with isolation and assignment of three new and two known monoterpene glucosides from the aerial parts of Origanum syriacum L.…”

Section: Introductionmentioning

confidence: 93%

Monoterpene glucosides from Origanum syriacum

et al. 2001

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Section: Introductionmentioning

confidence: 93%

Monoterpene glucosides from Origanum syriacum

et al. 2001

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“…Reversed-phase HPLC was found to be the more suitable chromatographic method for arbutin separation (Asaaf et al, 1986;Parejo et al, 2001;Pavlović et al, 2009). To our knowledge, there is no single validated HPLC method which was developed for the quantification of arbutin in many different plant extracts.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Determination of Arbutin and Hydroquinone in Different Plant Materials by HPLC

Rychlińska¹,

Nowak²

2012

Not Bot Hort Agrobot Cluj

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A simple, fast method of high-performance liquid chromatography for the determination and quantification of arbutin and hydroquinone in many different raw materials was developed and validated. The optimum conditions for the separation and detection of these two constituents were achieved on a LiChro-CARD 125-4 Superspher®100 RP-18 column with the water-methanol (gradient elution) mobile phase and recorded at 289 nm. The purities of peaks were verified by PDA analysis of impurities. The results of validation have shown that the HPLC method is stable and accurate for the simultaneous determination of arbutin and hydroquinone in extracts from various plants. The developed method gave a good sensitivity (LOD 1µg/ml for arbutin and 0.49 µg/ml for hydroquinone) with linearity R 2 >0.9993 (for both). The relative standard deviation of the method was less than 2.53% for intra-day assays and 3.23% for inter-day assay, the accuracy of the recovery test ranged from 98.96% to 106.4%. This method was used in comparative qualitative analysis of arbutin and hydroquinone in 16 different raw materials from families Lamiaceae, Ericacaeae, Saxifragaceae, Rosaceae. The content of arbutin in B. ciliata, B. cordifolia and Ledum palustre was examined for the first time.

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“…The substances that have a pigment inhibitory effect in the hyperpigmented lesions include retinoid, kojic acid, arbutin, hydroquinone, pentadecenoic acid, oleanolic acid, ursodeoxycholic acid, vitamin C, catechol, phenol, medimin C, and pinellia ternata extract 19 . Drugs that act by both suppression of melanocytes proliferation and melanogenesis include 4-isopropylcatechol, 4-hydroxyanisole, hydroquinone, monobenzylether of hydroquinone, monoethylether of hydroquinone, and azelaic acid [20][21][22][23][24] .…”

Section: Discussionmentioning

confidence: 99%

The Inhibitory Effect of Phytoclear-EL1 on Melanogenesis

et al. 2009

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Background: Phytoclear-EL1, an extract from Euphorbia lathyris seeds, has a whitening effect due to inhibition of tyrosinase activity. Objective: The purpose of this study was to investigate the inhibitory effect of phytoclear-EL1 on melanogenesis. Methods: Cultured B-16 melanoma cells and 30 human volunteers were used for in vitro and in vivo studies, respectively. Phytoclear-EL1 was added to the cultured B-16 melanoma cells, and applied to UVB-induced hyperpigmented lesions of human volunteers twice daily for 7 weeks. Changes in the number of B-16 melanoma cells, as well as changes in morphology, melanin content, and tyrosinase activity, were measured and then compared with the normal control and the 10 −3 M arbutin groups. Also, the effect of phytoclear-EL1 on UVB-induced hyperpigmented lesions was examined through subjective and objective measurements. Results: In the in vitro study (p＜0.05), the number, melanin content, and tyrosinase activity of cultured B-16 melanoma cells were decreased in the 5μg/ml phytoclear-EL1 group compared to the control group. On objective assessment with a chromameter, the 0.2% phytoclear-EL1 group had a larger difference in the mean L values before and 7 weeks after applying phytoclear-EL1 as compared to the other groups. On subjective assessment by both the researchers and subjects 7 weeks after applying experimental materials, the 0.2% phytoclear-EL1 group and positive control (3% arbutin) had higher scores than the placebo groups. These results demonstrated that phytoclear-EL1 in vivo and in vitro had an inhibitory effect on melano-

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Preliminary Study of Phenolic Glycosides fromOriganum majorana, Quantitative Estimation of Arbutin; Cytotoxic Activity of Hydroquinone

Cited by 45 publications

References 5 publications

Monoterpene glucosides from Origanum syriacum

Monoterpene glucosides from Origanum syriacum

Quantitative Determination of Arbutin and Hydroquinone in Different Plant Materials by HPLC

The Inhibitory Effect of Phytoclear-EL1 on Melanogenesis

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