Preliminary screening of &lt;em&gt;Waltheria indica&lt;/em&gt; (L.) plant for its anti-inflammatory activity

Chandekar, Amol; Vyas, Amber; Upamanyu, Neeraj; Tripathi, Atul; Agrawal, Surendra

doi:10.5138/09750185.2079

Cited by 2 publications

(2 citation statements)

References 16 publications

(26 reference statements)

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“…Pet ether, methanol leaf extracts of the W. indica, and aqueous extract of stems with leaves also showed dose-related inhibition of acute and chronic inflammation in Carrageenan induced edema. The effect may be due to inhibition of histamine, serotonin, bradykinin, prostaglandin, and cyclooxygenase (COX) products [9,70,71]. Significant reduction in the pellet weight of cotton pellet granuloma test models was also observed after treatment with methanolic leaf extracts [71].…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

Ethnobotanical, phytochemistry, and pharmacological property of Waltheria Indica Linn

Nirmala

Sridevi

2021

Futur J Pharm Sci

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Background In modern therapeutics, various human pathological disturbances were treated with the plant-based products. Waltheria indica Linn, a perennial herb, was commonly used in traditional medicine worldwide against various ailments such as cough, dysentery, diarrhea, bladder disorder, hemoptysis, inflammations, neuralgia, wounds, and ulcers. Main body The shrub was majorly distributed in tropical, subtropical regions and exists in many distinct local forms. Both the crude extracts and purified compounds from the whole plant and its parts showed wide pharmacological properties like antioxidant, analgesic, sedative, anti-bacterial, anti-fungal, and anti-parasitic. The phytochemical profile and traditional usage highlight the potency of the plant in the treatment of microbial infections and inflammatory diseases. Yet, additional studies are required for the confirmations of its traditional uses against other diseases. More detailed understanding of anti-cataract, anti-diabetics, asthma, anemia, and anti-cancer mechanism has to be explored. Though many research articles on the proposed plant are available, there has been a rising concern in the therapeutic property, especially on the alkaloids and flavonoids from this plant for drug design. Conclusion This article aims in a systematic and updated review on distribution, botany, traditional uses, phytocompounds, and relevant biological activities from each part of the plant. The information was collected from databases like PubMed, ScienceDirect, Web of Science, Google Scholar, books, dissertation, and reports via academic libraries that included more than 100 articles published since 1937. This ethnopharmacological study of the plant may create new insight into drug discovery to develop important novel leads against various biological targets.

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Section: Anti-inflammatory Activitymentioning

confidence: 99%

Ethnobotanical, phytochemistry, and pharmacological property of Waltheria Indica Linn

Nirmala

Sridevi

2021

Futur J Pharm Sci

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“…Extracts from leaves generated with hydrophobic solvents such as petrol ether or methanol, but also extracts from leafy stems generated with hydrophilic solvents such as water, showed dose-related inhibition of acute and chronic inflammation in carrageenan-induced edema. The effect is presumed to involve the inhibition of histamine, serotonin, bradykinin, prostaglandin and cyclooxygenase (COX) products [ 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Activity-Guided Characterization of COX-2 Inhibitory Compounds in Waltheria indica L. Extracts

et al. 2021

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Inflammation is the body’s response to infection or tissue injury in order to restore and maintain homeostasis. Prostaglandin E2 (PGE-2) derived from arachidonic acid (AA), via up-regulation of cyclooxygenase-2 (COX-2), is a key mediator of inflammation and can also be induced by several other factors including stress, chromosomal aberration, or environmental factors. Targeting prostaglandin production by inhibiting COX-2 is hence relevant for the successful resolution of inflammation. Waltheria indica L. is a traditional medicinal plant whose extracts have demonstrated COX-2 inhibitory properties. However, the compounds responsible for the activity remained unknown. For the preparation of extracts with effective anti-inflammatory properties, characterization of these substances is vital. In this work, we aimed to address this issue by characterizing the substances responsible for the COX-2 inhibitory activity in the extracts and generating prediction models to quantify the COX-2 inhibitory activity without biological testing. For this purpose, an extract was separated into fractions by means of centrifugal partition chromatography (CPC). The inhibitory potential of the fractions and extracts against the COX-2 enzyme was determined using a fluorometric COX-2 inhibition assay. The characterizations of compounds in the fractions with the highest COX-2 inhibitory activity were conducted by high resolution mass spectrometry (HPLC-MS/MS). It was found that these fractions contain alpha-linolenic acid, linoleic acid and oleic acid, identified and reported for the first time in Waltheria indica leaf extracts. After analyzing their contents in different Waltheria indica extracts, it could be demonstrated that these fatty acids are responsible for up to 41% of the COX-2 inhibition observed with Waltheria indica extract. Additional quantification of secondary metabolites in the extract fractions revealed that substances from the group of steroidal saponins and triterpenoid saponins also contribute to the COX-2 inhibitory activity. Based on the content of compounds contributing to COX-2 inhibition, two mathematical models were successfully developed, both of which had a root mean square error (RMSE) = 1.6% COX-2 inhibitory activity, demonstrating a high correspondence between predicted versus observed values. The results of the predictive models further suggested that the compounds contribute to COX-2 inhibition in the order linoleic acid > alpha linolenic acid > steroidal saponins > triterpenoid saponins. The characterization of substances contributing to COX-2 inhibition in this study enables a more targeted development of extraction processes to obtain Waltheria indica extracts with superior anti-inflammatory properties.

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Preliminary screening of <em>Waltheria indica</em> (L.) plant for its anti-inflammatory activity

Cited by 2 publications

References 16 publications

Ethnobotanical, phytochemistry, and pharmacological property of Waltheria Indica Linn

Ethnobotanical, phytochemistry, and pharmacological property of Waltheria Indica Linn

Activity-Guided Characterization of COX-2 Inhibitory Compounds in Waltheria indica L. Extracts

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