Preliminary results for 2‐D separation with high‐performance thin‐layer chromatography and pressurized planar electrochromatography

Abstract

Preliminary results of 2-D separation of test dye mixture using high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) are demonstrated. The advantage of 2-D HPTLC/PPEC separation is based on different separation selectivities obtained in both HPTLC and PPEC systems. HPTLC RP18 W plates of 5x20 cm from Merck were used in the investigations. In the first dimension, a HPTLC process was performed using 5 cm length of the plate and in the second dimension PPEC separation was obtained applying plate of 20 cm length. PPEC process followed prewetting the chromatographic plate with sample zones on it, which were partly separated after first dimensional (HPTLC) separation. In the experiments, the modified version of PPEC device for 20 cm long chromatographic plate and the reservoir for prewetting the adsorbent layer were applied.

A Modified Device for Pressurized Planar Electrochromatography and Preliminary Results with On-Line Sample Application

“…Despite these advantages, the potential of this method is not currently used in routine analysis, inter alia due to difficulties with the two stages of the PPEC process that precede the development of electrochromatograms: application of samples on the chromatographic plate and prewetting of an adsorbent layer of the chromatographic plate with a mobile phase solution. Our group has designed pre-wetting reservoirs with partitions, which protected the substance zone applied on the adsorbent layer against elution and dispersion during the prewetting process [6, 10, 11, 13]. Disadvantages of this approach are the risk of losing some of the sample and the need for too many manual operations [13].…”

Separation of Some Aromatic Amino Acid Enantiomers with Pressurized Planar Electrochromatography and TLC

“…The development of PPEC has been practically reported by three research groups [13][14][15][16][17][18][19][20][21][22][23][24][25] so far. In these papers, few distinct features of PPEC such as high efficiency, short time of separation process and different separation selectivity have been presented relative to thin-layer chromatography/high-performance thin-layer chromatography (TLC/HPTLC).…”

“…PPEC is relatively new mode of separation in which the mobile phase is driven into movement by electroosmotic effect in closed separation system [13][14][15][16][17][18][19][20][21][22][23][24][25]. This movement can be easily controlled by electric field strength and mobile phase composition (pH of the mobile phase, buffer concentration, organic solvent type and its concentration) [13][14][15][16][17][18][19][20].…”

Effect of mobile phase buffer pH on separation selectivity of some isoquinoline alkaloids in reversed-phase systems of Pressuriz

“…The ad van tages such as high per form ance, short sepa ra tion time, and sepa ra tion se lectiv ity dif fer ent than that of liq uid chro ma tog ra phy (LC) make this tech nique very at trac tive for ap pli ca tion in labo ra tory prac tice [17]. In ad di tion, PPEC ex peri ment pro ceeds in a closed sys tem, so no va por phase takes part in the sepa ra tion pro cess [2,3,5,6,7]. Be cause of the features men tioned, PPEC might also be highly suit able for the phar ma ceu ti cal and bio medi cal analy sis [2,8,9,12,19,20].…”