Preliminary Phytochemical Screening and Anti-Inflammatory Effect of the Aqueous Extract of Tabernaemontana Divaricata Flower in Wister Rats

Abstract

Objective: To determine the phytochemical analysis and anti-inflammatory effect of the aqueous extract of Tabernaemontanadivaricata flower in Wister Rats.Methods: The aqueous extract of fresh flowers of the plant Tabernaemontanadivaricata (family-Apocyanaceae) were prepared and analyzed for phytochemical constituents using standard methods. The anti-inflammatory activity of the plant extracts was examined with the help of rat models by taking diclofenac as a standard drug against T. divericata oil extract by using carrageenan induced rat paw edema method with the help of the instrument plethesmometer.Results: The present experiment shows the phytochemical analysis, anti-inflammatory activity of the aqueous extract of fresh flowers of the plant Tabernaemontanadivaricata (family-Apocyanaceae). Various phytochemical analysis revealed the presence of saponins, flavonoids, glycosides, steroids, proteins, phenols, amino acids and tannins. The oil extract of the plant showed much effective anti-inflammatory activity against the standard drug.Conclusion: It can be concluded aqueous extract of the flower of the plant Tabernaemontanadivaricata (family-Apocyanaceae) contain the high presence of phytochemicals. This extract was found to possess promising antimicrobial activity when compared with the standards.

Major Bioactive Alkaloids and Biological Activities of Tabernaemontana Species (Apocynaceae)

“…For centuries, several Tabernaemontana species, such as T. divaricata , T. catharinensis , T. crassa , and T. elegans , have been exploited in traditional and folk medicine for the treatment of illnesses and the prevention of diseases and ailments, such as sore throat, hypertension, abdominal pain, and pulmonary disease [ 9 , 20 , 70 , 71 , 72 ]. A variety of chemical compounds extracted from many parts of Tabernaemontana species reportedly contain alkaloids, which exhibit biological activities, such as antimicrobial, antioxidant, anti-inflammatory, anticholinesterase, antineurodegenerative, anticancer, antidiabetic, antivenom, larvicidal, antihypertensive, wound healing, analgesic, and many other activities [ 2 , 3 , 6 , 9 , 71 , 72 ]. The details of the isolated compounds and respective pharmacological properties of a few Tabernaemontana species are summarized in the following paragraphs.…”

“…Subsequently, these changes imitate the restoration of inflamed tissue and constrain additional damage to the organism [ 109 ]. Based on the characteristics of inflammation, this composite process is divided into two major types known as acute and chronic inflammation [ 72 , 109 , 113 ]. Acute inflammation occurs almost instantly, or a few hours following injury, and usually displays symptoms of redness, heat, and edema [ 72 ].…”

“…Based on the characteristics of inflammation, this composite process is divided into two major types known as acute and chronic inflammation [ 72 , 109 , 113 ]. Acute inflammation occurs almost instantly, or a few hours following injury, and usually displays symptoms of redness, heat, and edema [ 72 ]. Whereas, chronic inflammation occurs over an extended interval and is histologically characterized by the occurrence of lymphocytes and macrophages, which subsequently results in the development of fibrosis and necrosis tissue [ 114 ].…”

Qualitative and quantitative estimation of phyto constituents in different solvent extracts of leaf of Tabernaemontana divaricata

“…Previous studies have reported the phytochemistry and presence of associated chemicals like alkaloids, terpenoids, steroids, flavonoids, phenyl propanoids, phenolic acids and enzymes in the leaves, stems, and roots [3][4] . A variety of chemical compounds extracted from many parts of Tabernaemontana species reportedly contain alkaloids, which exhibit biological activities, such as antimicrobial, antioxidant [5][6] , anti-inflammatory,anticholinesterase, antineurodegenerative, anticancer, antidiabetic, antivenom, larvicidal, antihypertensive, wound healing, analgesic, astringent, antioxidantanxiolytic, anti diabetic and anticonvulsant [7][8][9] and many other activities [10][11][12][13][14][15] . In this study, qualitative and quantitative phytochemical analysis was performed to identify the presence of various phytoconstituents in different leaf extract of T. divericata with standard procedures.…”