Preliminary investigations of antioxidant, antityrosinase, acetylcholinesterase and anti-inflammatory activities of Actinodaphne species

Salleh, Wan Mohd Nuzul Hakimi Wan; Ahmad, Farediah

doi:10.12991/mpj.20162048388

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…These results are complying with the previous investigations [46], where it has been interpreted that the extract with higher phenolic content exhibits potential scavenging of DPPH free radical. Antioxidant activity of most medicinal plants is because of their redox properties due to the presence of different polyphenolic derivatives, which donates hydrogen, and acts as reducing agent and oxygen scavenger [26,47].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of carbon tetrachloride fraction of Actinodaphne angustifolia Nees (Lauraceae) leaf extract for antioxidant, cytotoxic, thrombolytic and antidiarrheal properties

et al. 2020

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Actinodaphne angustifolia Nees (Family: Lauraceae) is commonly used in folk medicine against urinary disorder and diabetes. The objective of the present study was to evaluate the antioxidant, cytotoxic, thrombolytic, and antidiarrheal activities of carbon tetrachloride (CCl4) fraction of leaves of A. angustifolia (CTFAA) in different experimental models. Antioxidant activity was evaluated by using qualitative and quantitative assays, while antidiarrheal effects assessed with castor oil-induced diarrheal models in mice. The clot lysis and brine shrimp lethality bioassay were used to investigate the thrombolytic and cytotoxic activities, respectively. CTFAA showed antioxidant effects in all qualitative and quantitative procedures. The fraction produced dose-dependent and significant (P<0.05 and P<0.01) activities in castor oil-induced diarrheal models. Moreover, CTFAA significantly (P<0.05) demonstrated a 15.29% clot lysis effect in the thrombolytic test, and the brine shrimp lethality assay LC50 value was 424.16 μg/ml bioassay. In conclusion, the current study showed CTFAA has significant antidiarrheal effects along with modest antioxidant and thrombolytic effects, and these data warrant further experiment to justify and include CTFAA as a supplement to mitigate the onset of diarrheal and cardiovascular disease.

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Section: Discussionmentioning

confidence: 99%

Evaluation of carbon tetrachloride fraction of Actinodaphne angustifolia Nees (Lauraceae) leaf extract for antioxidant, cytotoxic, thrombolytic and antidiarrheal properties

et al. 2020

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“…L-DOPA was used as the substrate and mushroom tyrosinase as the enzyme. [16][17] The assays were conducted in 96-well microtiter plate, and Epoch Biotek reader was used to measure the absorbance at 475 nm. Mushroom tyrosinase enzyme (MTE) was prepared from the stock solution to 480 units/ mL in PBS.…”

Section: Tyrosinase Inhibitory Activitymentioning

confidence: 99%

Phytochemicals and Tyrosinase Inhibitory Activity from Piper caninum and Piper magnibaccum

et al. 2019

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Background: Piper species are aromatic plants used as spices in the kitchen, but their secondary metabolites have also shown biological effects on human health. In traditional medicine, Piper species have been used worldwide to treat several diseases such as urological problems, skin, liver and stomach ailments, for wound healing, and as antipyretic and anti-inflammatory agents. In the present study, we attempted to isolate the phytochemicals from Piper caninum and Piper magnibaccum and evaluate their tyrosinase inhibitory activity. Methods: Phytochemical constituents of the extracts were investigated using various chromatographic and spectroscopic methods. The structures of the isolated phytochemicals were established by analysis of their spectroscopic data, as compared to that of reported data. Tyrosinase inhibitory activity was also tested on the extracts and selected compounds using mushroom tyrosinase as the enzyme. Results: Fractionation and purification of the extracts of Piper caninum and Piper magnibaccum afforded nine known compounds which were cepharanone A (1), cepharadione A (2), aristolactam AII (3), 5,7-dimethoxyflavone (4), 24-methylenecycloartan-3-one (5), β-sitosterol (6), piperumbellactam A (7), 24S-ethylcholesta-5,22,25-trien-3β-ol (8) and stigmast-3,6-dione (9). Ethyl acetate extracts from leaves of P. magnibaccum gave the highest inhibition value at 48.35%, while the tested compounds displayed weak tyrosinase activity compared to the positive control, kojic acid. Conclusion: These phytochemical results suggested that the extracts could assist as a potential source of bioactive compounds. Further research is needed in which the extract could possibly be exploited for pharmaceutical use.

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“…The essential oil of A. pruinosa demonstrated significant activity on DPPH (IC50 85.6 μg/mL), phenolic content (190.2 mg GA/g), and lipoxygenase (IC50 85.2 μM) assays. In addition, antioxidant, antityrosinase, acetylcholinesterase, and anti-inflammatory activities of the leaves and stem bark extracts have also been reported [8]. The methanolic leaves extract of A. pruinosa have shown activity on DPPH (IC50 176.8 μg/mL), ABTS (IC50 224.5 μg/mL), and phenolic content (42.8 mg gallic acid/g).…”

Section: Introductionmentioning

confidence: 98%

Chemical constituents of the leaves of Actinodaphne pruinosa

Salleh¹,

Shakri²,

Nafiah³

et al. 2022

Bull. Chem. Soc. Eth.

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ABSTRACT. This study was designed to investigate the chemical constituents from Actinodaphne pruinosa growing in Malaysia. A phytochemical investigation of the leaves part resulted in the isolation of boldine (1), norboldine (2), laurotetanine (3), reticuline (4), syringaresinol (5), lupeol (6), and taraxerol (7). The structures of the isolated phytochemicals were established by analysis of their spectroscopic data, as well as the comparison with that of reported data. Notably, this is the first time to report the isolation and structural elucidation of the constituents from the leaves part of A. pruinosa. KEY WORDS: Actinodaphne pruinosa, Aporphine, Lauraceae, Phytochemical Bull. Chem. Soc. Ethiop. 2022, 36(4), 963-969. DOI: https://dx.doi.org/10.4314/bcse.v36i4.20

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Preliminary investigations of antioxidant, antityrosinase, acetylcholinesterase and anti-inflammatory activities of Actinodaphne species

Cited by 4 publications

References 24 publications

Evaluation of carbon tetrachloride fraction of Actinodaphne angustifolia Nees (Lauraceae) leaf extract for antioxidant, cytotoxic, thrombolytic and antidiarrheal properties

Evaluation of carbon tetrachloride fraction of Actinodaphne angustifolia Nees (Lauraceae) leaf extract for antioxidant, cytotoxic, thrombolytic and antidiarrheal properties

Phytochemicals and Tyrosinase Inhibitory Activity from Piper caninum and Piper magnibaccum

Chemical constituents of the leaves of Actinodaphne pruinosa

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