Preliminary investigations into solutal flow about growing tetragonal lysozyme crystals

Pusey, Marc L.; Witherow, William K.; Naumann, Robert J.

doi:10.1016/0022-0248(88)90304-1

Cited by 118 publications

(90 citation statements)

References 13 publications

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“…In the absence of gravity, there is no convective mixing and thus solute transport is entirely dominated by diffusion (Pusey, Witherow & Nauman, 1988). Because of the large size of the protein molecules and their low diffusivity, concentration gradients form in the neighborhood of the growing crystal.…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Thaumatin Crystals Grown on Earth and in Microgravity

Lorber²,

Giegé³

et al. 1997

Acta Cryst D

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The protein thaumatin was studied as a model macromolecule for crystallization in microgravity-environment experiments conducted on two US Space Shuttle missions (USML-2 and LMS). In this investigation, we have evaluated and compared the quality of space-and earth-grown thaumatin crystals using X-ray diffraction analyses, and characterized them according to crystal size, diffraction resolution limit and mosaicity. Two different approaches for growing thaumatin crystals in the microgravity environment, dialysis and liquid-liquid diffusion, were employed as a joint experiment by our two investigative teams. Thaumatin crystals grown in a microgravity environment were generally larger in volume and the total number of crystals was less, relative to crystals grown on earth. They diffracted to significantly higher resolution and with improved diffraction (/,,)

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Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Thaumatin Crystals Grown on Earth and in Microgravity

Lorber²,

Giegé³

et al. 1997

Acta Cryst D

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“…[1,3,6,7,12] and this work), the unit cell sized growth steps found on the lysozyme crystal faces [1], and the large degree of aggregation which has been observed, even below the saturation concentration, by light scattering intensity and dialysis kinetics measurements [16,17]. Re- …”

Section: Discussionmentioning

confidence: 99%

The effects of temperature and NaCl concentration on tetragonal lysozyme face growth rates

Forsythe¹,

Pusey²

1994

Journal of Crystal Growth

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“…It was thought that the shear force arising from the fluid flow impeded the protein growth [9]. However, M. L. Grant and D. A. Saville [11] studied the role of transport phenomena in the protein growth and found that the shear force arising from the fluid flow could not alter the state of the protein near the crystal surface.…”

Section: Introductionmentioning

confidence: 99%

“…Mass transfer and crystal growth kinetics have been studied for overcoming this difficulty [1]. Crystallization is inherently unable to maintain a state of equilibrium as it creates a solute concentration gradient which may lead to a buoyancy-driven flow in the gravitational field [2], which is thought to be detrimental to protein crystal growth, because the forced flow will influence the steady-state protein concentration gradient around the growing protein crystal and destroy its mass filtering capability [3]. Space was thought as a perfect environment for the production of high quality protein crystal because the sedimentation movement and convective flow due to gravity are negligible under microgravity conditions [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Effects of forced solution flow on lysozyme crystal growth

Yu¹,

Liu

Wang

et al. 2010

Cryst. Res. Technol.

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In order to study quantitatively the effects of forced solution on crystal growth, we designed a new set of experimental equipment, in particular, a microchannel mixer was used as crystallization container so that the consumption of protein samples was much reduced and thus an exact syringe pump could be used for precise control of the flow rates. Since the mixer's section was designed to be rectangular, the solution velocity in its center was steady and constant, and thus repeatable experiments were facilitated. Experimental results showed that the effects of forced solution on protein crystal growth were different under different levels of supersaturation, and new results were obtained for cases of high supersaturation. When the supersaturation is σ = 2.3, with increasing flow rates the growth rates of the lysozyme crystal's (110) face hardly change when the flow rates are lower than 1300 μm/s, and decrease quickly afterwards. When the flow rate reaches 2000 μm/s, the crystal nearly ceases to grow. When the supersaturation is σ = 2.7, with increasing flow rates the (110) face growth rates increase at the beginning then reach the maximum values at 1700 μm/s -1900 μm/s and decrease afterwards, approaching zero or so when the flow rate reaches 12000 μm/s. The higher the supersaturation, the larger the flow rate at which the crystal ceases to grow.

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Preliminary investigations into solutal flow about growing tetragonal lysozyme crystals

Cited by 118 publications

References 13 publications

Comparative Analysis of Thaumatin Crystals Grown on Earth and in Microgravity

Comparative Analysis of Thaumatin Crystals Grown on Earth and in Microgravity

The effects of temperature and NaCl concentration on tetragonal lysozyme face growth rates

Effects of forced solution flow on lysozyme crystal growth

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