Preliminary evaluation of UF-5000 Body Fluid Mode for automated cerebrospinal fluid cell counting

Lack of harmonization in high fluorescent cell automated counts with body fluids mode in ascitic, pleural, synovial, and cerebrospinal fluids

“…The intercepts were comprised between −4.9 for MN‐BF and 12.0 for PMN‐BF using BC‐BF (Table ). The bias calculated with Bland‐Altman plots was then comprised between −97.0% for EO‐BF using the XN‐BF and 40.3% for PMN‐BF using BC‐BF …”

“…Cell counting and classification in body fluids (BFs; ascitic [AF], pleural [PF], synovial [SF], and cerebrospinal [CSF]) has become very popular in recent times due to enhanced availability of new commercial instrumentation . Carried out using suitable analyzers, automated cell count can now be regarded as a useful screening, since is adequately precise, may substantially reduce the turnaround time (TAT), is available 24 hours a day, and allows better standardization of the analytical process …”

“…Although all hematological analyzers (also referred to as “hemocytometers”) equipped with the BF‐mode can generate both quantitative and qualitative measures, these parameters differ among the various commercial analyzers, being mostly instrument‐dependent. Quantitative parameters may include total cell count (TC), leukocyte count (WBC) and, sometimes, differential cell count capable to discriminate between polymorphonuclear (PMN) and mononuclear (MN) leukocytes . The two hematological analyzers of Sysmex XN series (Sysmex, Kobe Japan) and the Mindray BC‐6800 (Mindray, Shenzhen, China) are equipped with a BF‐mode channel dedicated to cell count and classification, which is based on optical fluorescence reading, selective lysis of red blood cells (RBCs), along with specific hardware and fluidics.…”

“…For both XN‐BF and BC‐BF modes, the number of cells classified as HF‐BF is significantly correlated with the presence of mesothelial and/or various neoplastic cells . According to the origin of the BF, the presence of a variable number of cells classified as HF‐BF can then suggest microscopic revision of the sample …”

Relationship of Serum Fetuin A with Metabolic and Clinical Parameters in German Children and Adolescents with Type 1 Diabetes

“…However, previous data on fetuin A and atherosclerosis in non-T1D cohorts are inconsistent: both low and high fetuin A concentrations were associated with cardiovascular disease and mortality risk [21-23]. Some authors suggested that fetuin A plays a protective role against vascular calcification and atherosclerosis [23, 59, 60], while others found that higher fetuin A levels are associated with arterial stiffness, increased intima media thickness [41, 43, 61, 62], and an increased risk of peripheral arterial disease, myocardial infarction, and ischemic stroke [20, 63, 64]. Comorbidity and late complication rates were low and not related to fetuin A levels, e.g., we did not observe any case with diabetic retinopathy in our cohort, which may be either due to the fact that the number of patients was too low, that patients were too young to observe late complications, or that fetuin A is not a reliable marker for increased complications in young T1D patients.…”